David Shechter

Recent Posts

Homogenizers for Cannabis: How To Deliver More Consistent & Uniform Particle Size

Posted by David Shechter on Nov 29, 2018 11:30:00 AM

homogenizers for cannabisIf you’re looking into cannabis processing options, homogenization might seem a bit complicated at first. While there are certain elements to homogenization that are technical, homogenization is actually a pretty simple process! When it comes to particle size reduction, homogenizers work to combine two or more ingredients in a uniform manner. Since homogenization is mechanical, it doesn’t involve adding preservatives or other materials to the mixture. Thanks to pressure and other forces, particles become uniformly dispersed in the homogenization process.

When it comes to cannabis products, one of the best benefits a homogenizer can offer is how they deliver more consistent and uniform particle sizes in their dispersions. Here are the top three reasons why this should matter to you:

1. No More “Hot” and “Cold” Spots

As mentioned above, when you homogenize two or more ingredients, those particles are reduced to a small size and uniformly dispersed throughout the mixture. So without homogenization, there’s not a good way to determine where the cannabinoids are in your products. Therefore, you may have one item that is chock full of cannabinoids while the next item doesn’t have any. In fact, a recent study found 31% of cannabis products tested did not contain the amount of cannabinoids their labels claimed. That’s an unacceptable number in any industry, but can be particularly embarrassing for companies that are looking to establish themselves in the relatively new commercial cannabis industry.

2. Increased Drug Efficacy

Next, a smaller particle size will have a greater total surface area which can increase the rates of dissolution and absorption of any active ingredient. Therefore when working with cannabis, you need to ensure your products have small, uniform particle size for a greater absorption rate and there’s no better way to do this than using a homogenizer. Homogenizers can help you reach your target particle size reliably plus you can run a filter through the end product to see that all particles are the target size. Further, smaller particles can increase bioavailability by up to 75% by increasing the surface area and you can actually reduce the active ingredient required to achieve the same effect.

3. Product Appearance

While this doesn’t apply to cannabis products alone, a smaller, more uniform particle size can be beneficial for aesthetic reasons. When particles are homogenized properly, the end product can appear smoother and more homogenous. Therefore, when looking to add cannabis to lotions, food products, and other items, having a beautiful product can make all the difference when it comes to sales.

Final Thoughts

There’s a long history of businesses across all industries using homogenizers to guarantee uniformity in their product, which is something that everyone in the cannabis industry should want when creating new products. Plus, consumers want to associate with brands and products they can trust. So when you use high-pressure homogenizers in your process, you'll be keeping the promises you already make to your customers.

To learn more about CannaBEE, our homogenizer solutions customized for cannabis, check out this page for more information or contact us today for a free quote:

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How to Improve RNA Isolation with Homogenization

Posted by David Shechter on Nov 15, 2018 11:30:00 AM

rna-isolationRNA isolation is, unsurprisingly, a delicate and complicated process that requires diligent execution and precise application. Indeed, even seemingly small mistakes can completely ruin an attempt to extract, isolate, and study RNA samples. With that in mind, today we’re here to explain how homogenization will help improve RNA isolation –– while also sharing a few best practices all professionals can use to boost their results when working with RNA. Check them out below:

Sample Collection and Preservation

Researchers value RNA analysis in no small part because studying RNA can help explain how genes function and are expressed. Regardless of the aim of a given experiment or project though, collecting and preserving samples in the right way is essential to generating viable results with RNA. Some tissues are notoriously difficult to extract RNA from (like bones or roots) and if samples aren’t preserved properly before homogenization, the results of a study can also be affected. As such, researchers should strive to either 1) conduct homogenization immediately following sample collection, which may not always be possible, or 2) freeze the samples using a substance like liquid nitrogen for temporary containment until homogenization can be performed. Note here that thawing frozen samples before homogenization will also affect the outcome of an experiment.

Benefits of Homogenization

The good news about utilizing homogenizers in regard to RNA extraction is that they eliminate the potential of heat production –– since high-pressure homogenizers operate so quickly. In addition, homogenizers are scalable and compatible with a wide array of sample sizes and types. Even if a samples has cell walls that are hard, or when other conditions aren’t ideal, homogenizers can still assist researchers in achieving meaningful yields. Lastly, homogenizers are key to separating samples from RNase, which as we’ll explain below, will significantly alter RNA samples and often render them unusable.

Preventing RNase Exposure

Ribonuclease or RNase, presents a problem for researchers looking to study intact RNA. RNase is an enzyme that catalyzes the degradation of RNA into smaller pieces. This is obviously something researchers want to avoid. Unfortunately, RNase is more or less everywhere, which means that it’s easy to expose a sample to it through lab equipment or on the surface of gloves. Professionals can use special cleaning solutions to ensure their equipment doesn’t spoil their experiment thanks to RNase exposure–– and should change their gloves often throughout their work. Remember also that proper storage at the end of the isolation procedure is also imperative to prevent any outside factors influencing results. RNA is generally stored at low temperatures –– either -20 Cº or -80 Cº depending on how long the storage will last. 

Final Thoughts

With the right equipment and by following guidelines and best practices to the letter, researchers can glean meaningful RNA yields under adverse conditions. At BEE International, we’re passionate about providing scalable, highly effective homogenization solutions for our business partners. We strive to offer the finest products –– and customer service –– in the industry. Don’t just take our word for it, though. Contact us today!

Plus, to get a firsthand look at how our homogenizers enable efficient and consistent particle-size reduction, check out our free eBook here:

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Why We Love High Pressure Homogenizers (And You Should, Too!)

Posted by David Shechter on Aug 30, 2018 11:30:00 AM

Love High Pressure HomogenizersIf you were asked about some of your favorite products, a high-pressure homogenizer may not make the list. However, as we’ve discussed in a previous blog, the homogenization process produces a lot of products you most likely use and consume in your daily life. That’s one of the reasons we here at BEE International love homogenizers…but there are more! Let’s take a look at some of the most popular uses of high-pressure homogenization and the benefits they offer consumers.

Particle Size Reduction

High-pressure homogenization utilizes mechanical force to break down particles or droplets in a liquid into smaller and more uniform sizes, resulting in a dispersion, which is where fine particles of one substance are scattered throughout another substance. Dispersions can be further classified into solutions, suspensions and colloids, a mixture somewhere between a solution and a suspension.

Some high-pressure homogenizers can reduce particle size to as small as a single-digit nanometer. Plus, they ensure the composition is equal throughout, and the overall molecular makeup of the sample isn’t compromised. Many high-pressure homogenizers are able to produce emulsions, dispersions and suspensions at particle sizes of 100 µm or higher, and a select few can achieve a size of down to 0.1 µm. Key benefits of particle size reduction include:

  • Increased dissolution rate.

  • Improved drug delivery.

  • Controlled rate of reaction.

  • Increased density.

  • Consistent texture and product appearance.

  • Enhanced sedimentation stability.

  • Improved mineral processing.


Important Applications

Food and Beverages

Though one of the most well-known uses of high-pressure homogenization in the food and beverage industry is for milk, it is also widely used in the manufacture of sauces, spreads, salad dressings, fruit juices and concentrates, fish and meat pâté, sweeteners, baby food, egg product, other dairy products and more. It helps companies utilize to efficiently, cost-effectively and safely produce their goods. Food homogenizers also aid in the delivery of a product with uniform texture, flavoring and taste through a scalable process that allows for a longer period of freshness than food comprised of larger particles.

Pharmaceuticals, Chemicals and Biofuels

High-pressure homogenizers are used for numerous products from the chemical and biotechnology industries and products such as pharmaceutical tablet coatings, vaccinations and creams. They can be used to create cost effective medicines, modify microorganisms, synthesize new chemicals and develop alternative biofuels.

Cosmetic Creams and Lotions

Due to its ability to provide potent particle size reduction, enriched texture and consistency and strengthened emulsion stability, high-pressure homogenization is a preferred method for improving the production of cosmetic creams, lotions and other cosmetic products. It is especially beneficial for beauty creams and moisturizing lotions because they have to have a combination of the right texture and consistency in order for the skin to be able to effectively absorb them.

RNA Extraction

 Utilizing homogenization for RNA extraction enables processing to be effectively and repetitively processed in only seconds, thereby eliminating heat production. Its force and high pressure produce consistent and uniform samples, and it can process both small and large samples. Plus, homogenizers are scalable and compatible with many sample types, including those with tough cell walls. Using a powerful chaotropic salt solution, RNA extraction purifies RNA from biological samples by quickly disrupting cells or tissue to release the nucleic acids from the cells. These released RNAs are valuable in providing details on which genes are expressed (used to make products), to what degree the genes are active, how they work to regulate each other via small, regulatory RNAs and which structural RNAs (i.e. rRNAs, mRNAs and tRNAs) are produced.

Oil Emulsification

 Due to the fact that water and oil are immiscible, powerful mixing is essential to conducting oil emulsification. Therefore, specialized machines such as high-pressure homogenizers are preferred because they not only impart high shear but also are able to reduce particle sizes much more efficiently than other blending and emulsification methods. They are used in a wide variety of research and scientific uses and by many industries that require the use of active ingredients because they reduce active ingredients to a small enough size to ensure a thorough dispersion throughout the medium produced. Additional benefits of high-pressure homogenization for oil emulsification include production of stable emulsions, improved product consistency, shelf-life, texture, color and flavor and the ability to overcome the resistance caused by two immiscible liquids.

BEE International: Our Love for Our Products Shows in the Way We Treat Our Customers

BEE International offers homogenizers that are both high-quality and reliable. Our line of equipment is suitable for virtually every industry and can work in just about any environment. Contact us today to learn how our products can maximize your homogenization processes.

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The Ultimate Cheat Sheet for Choosing a Cell Homogenizer

Posted by David Shechter on Aug 16, 2018 11:30:00 AM

Businessman is standing in front of two direction signs.If you attended high school before laptops and tablet computers replaced books and used a landline to make phone calls, you’ve probably heard of CliffsNotes. Often used to help students figure out the intricacies of a novel that was required reading for English class, these handy pamphlets were a sort of a study guide highlighting the work’s key points.

In this blog, we’re offering you a CliffNotes version of tips for selecting a cell homogenizer that best meets your business needs. We’ll outline cell homogenization, often referred to as cell lysis, offer key factors for how best to achieve it and provide some benefits on it.

What is Cell Lysis?

Cell lysis, which takes place in biotech, pharmaceutical, food, cosmetic and chemical laboratories on a regular basis, is the rupture of the cell membrane resulting in the release of cell contents and the subsequent death of the cell. Research scientists and laboratory technicians disrupt the cell’s structure to extract organelles, proteins, DNA or mRNA. 

Cell lysis can be achieved through mechanical or chemical methods. Mechanical cell lysis techniques include high shear mixing, traditional homogenization, sonication, grinding, freeze/thaw cycles and high-pressure homogenization. Chemical cell lysis techniques include osmotic lysis and the use of detergents, chelating agents or chaotropic agents.

In order for cell lysis to be performed successfully, the following factors should be present:

  • Intracellular proteins should be accessible for extraction and solubilization.
  • The process should be flexible, as different cells require different cell lysis strategies.
  • The technique should result in a high yield in a short time.
  • The process should be relatively easy to perform.
  • Results should be consistently reproducible and scalable.

 

How Homogenization Helps

Of the methods used to achieve cell lysis, the most widely used is homogenization, which offers a multitude of benefits for production laboratories and the consumers who buy their products. It is especially useful for small volumes of cells and/or cultured ones and involves the use of shearing force on the cell. By forcing a small cell through an even smaller-sized orifice, homogenization removes the outer layer and lyses the cell. Some cell lysis products use utilize shear, and others employ sonic cavitation. However, the best choice when purchasing a homogenizer for cell lysis is one that uses shear, cavitation and impact and lets the user adjust the relative amount of each force for different applications.

The homogenizer you choose for cell lysis should be scalable to allow for customization in pressure and intensity, resulting in both cost and time efficiency for the user. It also must accommodate for multiple cell types and adjust for various sample sizes. Other factors to consider when selecting the right cell homogenizer are the force required to produce the highest yield in the least amount of time and the volume, efficiency, stability and sanitary standards compliance of the product. Advantages of using this type of homogenizer are:

  • Accessibility of intracellular proteins.
  • Built-in process flexibility.
  • Ease-of-use and low learning curve.
  • Reproducible and scalable results.
  • Various sample size processing.
  • Ability to disrupt all cell types.
  • Better results in fewer passes.

BEEI International: Bringing the Best Homogenizers to You

BEE International offers numerous high-quality homogenizers to meet the needs of any industry for cell lysis. Our laboratory-grade models include the Nano DeBEEMicro DeBEE and Mini DeBEE, all of which are easy to use, provide consistent and reliable results and easily scale up for full scale production. Plus, they offer maximum experimentation capabilities and the ability to change flow, pressure, shear and impact settings. Contact us today to learn more about our full line of homogenizers.

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Making the Right Choice: Homogenizer Manufacturers

Posted by David Shechter on Jul 31, 2018 11:30:00 AM

choosing high pressure homogenizerShopping for certain products is easy. If you want a fast food hamburger, you have a few well-known establishments from which to choose. If you’re looking to purchase some products to stain a deck or tend to weeds in your lawn, you most likely go to your local hardware store or one of a couple bigger, nationwide home improvement stores.

Investing in a more expensive product often requires more research and comparison. Usually, you can read customer reviews online or find ratings published by consumer organizations. When purchasing a homogenizer, however, it may be harder for you to obtain the advice needed to make the right decision.

First, let’s do a little refresher on homogenization. The primary methods used for homogenization include ultrasonic, mechanical and high-pressure, and homogenizers are used across multiple industries, such as:

  • Pharmaceutical: vaccines, antibiotics, cancer treatment and medicinal creams.
  • Food and Beverage: mayonnaise, ice cream, cream liqueurs, salad dressings, dairy products and more.
  • Biotech: creation of cost-effective medicines, modification of microorganisms, synthesization of chemicals and development of alternative biofuels.

Based on these details, the first step in finding a homogenizer manufacturer in asking yourself the following questions:

  1. Why do I need one?
  2. What differentiates one homogenizer from similar ones on the market?
  3. Does my product require a specific particle size?
  4. Which accessories are recommended and/or required for optimal performance?
  5. What motor power does my product require?
  6. Do I have a desired tube capacity and/or mixing speed?

By understanding the available options and what can best help you accomplish your specific homogenization goals, you are better equipped to select a manufacturer to meet your specific needs. It’s important to choose a manufacturer who will work with you to customize a homogenizer that delivers the best possible results. Some companies are able to customize the forces in their homogenizers to suit your application. Some even produce separate homogenizers for each phase of drug development.

Consistency is essential in homogenization, so employing the services of a manufacturer who aids you in reproducing reliable results is imperative. They should offer a homogenizer that is easy to scale and enables the product developer to quickly apply a wide variety of mixing techniques to a formulation. The manufacturer should have a reputation for reliability, offer customer referrals and provide you with at least once demonstration of their product(s).

Once you’ve decided on a manufacturer from which to purchase your homogenizer, factors to consider when choosing a brand and model include:

  • Sample types.
  • Maximum operating pressure. 
  • Mixing speed.
  • Processing setup. 
  • Processing range. 

 

BEE International: The Right Choice for the Best Reproducible Results

Our technology achieves superior results for nano emulsions, cell lysis, uniform particle reduction and related applications, and we offer homogenizers that are high-quality, customizable and reliable. Plus, we provide unparalleled customer service from demo to purchase and beyond. Contact us today for more information on our entire line of homogenization equipment.

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Summer, Sunscreen, and Homogenization

Posted by David Shechter on Jun 13, 2018 11:30:00 AM

bee1For many people across the United States, the winter of 2017 was one of the coldest on record. Residents of the East Coast had to deal with multiple Nor'easters, shutting down airports and wreaking havoc on roadways.

For most of us, summer and the high temperatures that typically accompany it are definitely welcome. Kids are out of school, baseball games are being played and swimming pools are being opened and prepped for lazy summer days.

With the arrival of summer, though, comes a reminder about the dangers of getting too much sun. More people are diagnosed with skin cancer annually in the U.S. than all other cancers combined, and the annual cost for treating skin cancers in this country is an estimated $8.1 billion.

Skin cancer may be prevented or at least minimized through the regular use of sunscreen. In fact, scientific evidence supports the benefits of using sunscreen to reduce short- and long-term damage to the skin from the sun’s rays.  

High-pressure homogenization is a preferred method for improving the production of cosmetic creams or lotions, including sunscreen, because it offers the following benefits:

Potent Particle Size Reduction

By forcing a sample through a narrow space while applying high pressure and multiple mechanical forces, including cavitation, shear, impact and turbulence, high-pressure homogenization more effectively decreases particle size. This reduction, or micronization, creates a more homogenous mixture, provides better active ingredient dispersion and stabilizes product emulsions. It’s especially beneficial for creams, lotions and other cosmetic products because of the positive effect on the quality of these items. 

Enriched Texture and Consistency

High-pressure homogenization is often preferred over other methods because of it’s capability to create a product that is uniformly mixed. This leads to smoother texture and enhanced consistency, which is essential for beauty creams and moisturizing lotions because they have to have a combination of the right texture and consistency in order for the skin to be able to effectively absorb them. 

Strengthened Emulsion Stability

Semi-solid products such as lotions and creams are comprised of emulsions, which are a mixture of two immiscible liquids. Micronization of droplets acts to eliminate the separation of water phase of homogenization, resulting in a more permanent mixture of oil and water. This permanence extends the shelf life of these semi-solid products and improves their appearance through consistent coloring.

BEEI: Homogenizers for High-quality Creams and Lotions

If you’re searching for a cosmetic homogenizer to meet your laboratory and/or production needs, make sure you choose one that’s high quality and includes a combination of capabilities. Whether your product is a cream, lotion, makeup or personal care item, opting for a customizable high-pressure homogenizer will only improve and enhance its effectiveness.

Contact us today to learn more about our full line of homogenizers and what they can do for you.

For more information on homogenization and how to achieve efficient and consistent particle size reduction, download our FREE eBook:

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Pharmaceutical Equipment Manufacturers: Five Questions to Ask Before Purchasing a Homogenizer

Posted by David Shechter on May 21, 2018 11:00:00 AM

pharmaceutical equipment manufacturers homogenizerBuying an automobile or house may be a complex process, but you often want to perform detailed research before making such a substantial purchase. You want to ensure your purchase has the capabilities you require, fits within your budget and offers a good investment.

For manufacturers of pharmaceutical equipment, doing research before buying a homogenizer or any other sizable item necessary for production is key to finding the right one. Though this process many seem time-consuming, you’ll end up with a product that best fits your needs and gives you the most benefits. Following are ten questions to ask before buying a homogenizer:

Why do I need one?

Though homogenizers are valuable tools for manufacturers in multiple industries, different models typically address different needs. For instance, maybe you need a homogenizer to mass-manufacture a product that has been approved for market. Or, perhaps you’re conducting research and development and need to disrupt yeast cells. At BEE International, we produce separate homogenizers for each phase of drug development. Make sure the product you choose is best suited to your specific business needs.

What differentiates one homogenizer from similar ones on the market?

In your research for a homogenizer, be sure to ask each manufacturer about their product’s specific capabilities and benefits. Their customer support personnel should be able to articulate any unique functions and/or specifications their products have and define ways it may be more cost-effective than competing products.

Does my product require a specific particle size?

To purchase a homogenizer which provides maximum laboratory output, you should ensure you know the required consistency in texture and size for your product to achieve safety and efficacy. Most pharmaceutical products, including vaccines, inhalants and anesthetics, need to be synthesized using a specific particle size. Many high-pressure homogenizers are able to produce emulsions, dispersions and suspensions at particle sizes of 100 µm or higher, but only a select few can achieve a size of down to 0.1 µm.

Which accessories are recommended and/or required for optimal performance?

Many times, a homogenizer without a lot of bells and whistles will perform the job for which you selected it well. However, some accessories are designed to offer benefits such as time savings, improved process accuracy and enhanced process efficiency. For example, deflector heads can facilitate homogenization of viscous material, generator probes can prevent cross-contamination and pre-assembled bead kits can make the process much easier. Check with each manufacturer to see if any specific accessories are needed for their homogenizing product to run at its highest possible level.

Do you have a demo machine we can test to see if your product meets our needs?

Before you purchase any homogenizer from any manufacturer, it’s important to try each one out to make sure it’s the best fit for your laboratory. For instance, if you handle high volumes of samples, you should try a homogenizer with a high motor capacity for optimal processing efficiency. If you have a desired mixing speed, you should examine homogenizers that let you easily achieve and maintain this speed or change it as necessary.

Other questions to ask when looking to purchase a homogenizer should center around company reputation, product warranties, level of customer service after purchase, user training and education, output capacity and ease of use. Taking the time to select the homogenizer best suited to your needs will be worth the effort.

BEE International: Trust Us with Your Homogenization Needs

At Bee International, we have extensive experience in the challenges our pharmaceutical customers face as they transition from concept, R&D, clinical trials and all-important FDA approval to manufacturing. Plus, we’re trusted by pharmaceutical researchers and laboratory managers around the world. Contact us today to learn more about our complete line of homogenizers.

For more information on the advantages of BEE homogenizers for the pharmaceutical industry, download our FREE eBook:

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Lessons on Selecting a Laboratory Homogenizer

Posted by David Shechter on Mar 26, 2018 2:10:06 PM

Top view of businessman legs choosing his way-747702-edited.jpegMost of us have experienced the activity (or drudgery) of purchasing an automobile, and the process of selecting one can be extremely tedious. There are a wide array of manufacturers, models, colors and sizes from which to choose and even more options within those choices.

Fortunately, selecting a laboratory homogenizer that best meets your business needs doesn’t have to be as complicated. If you purchase a model that accommodates your required applications, it should be able to serve your homogenization needs for the long-term. Even though all laboratory homogenizers can accomplish emulsions, cell disruptions and more, understanding the commercially available homogenizing methods can make it simpler to determine the right homogenizer for a particular application.

Main Methods of Homogenization

The primary homogenization methods include ultrasonic, mechanical and high-pressure. Ultrasonic homogenizers, or sonicators, are used in cell lysis and particle size reduction applications. They are best utilized for samples which are incredibly difficult to disrupt, including tough animal tissues, spores, soil samples and liposomes. Although they are often used to process small samples in suspension and do not require pre-grinding or cutting, ultrasonic homogenizers generate heat, which can harm temperature-sensitive samples.

Mechanical homogenizers work by using pressure (shearing) and/or force to disrupt cells. They produce consistent results quickly and are able to alter a sample’s physical structure while maintaining its chemical composition. The mechanical homogenization method provides for easy scalability and especially well-suited for animal tissues and cells.

Probably the most commonly utilized method, high-pressure homogenizers have the capability to lyse cells and create emulsions, thereby enabling a longer product shelf life and an even distribution of active ingredients. They are one of the most efficient fluid processing systems for cell disruption and particle size reduction, especially for applications which require minimum particle sizes and homogenous mixtures. High-pressure homogenizers enable the study of tissues, cells and more.

Factors to consider when choosing a brand and model of laboratory homogenizer include:

  • Sample types.
  • Maximum operating pressure. 
  • Mixing speed. 
  • Processing setup. 
  • Processing range.  

Benefits of BEE International

At BEE International, our laboratory high-pressure homogenizers were designed to provide formulation scientists with more experimentation options and capabilities for emulsions, dispersions, cell rupture and liposomes. Plus, they offer a multitude of benefits and features, such as operating pressures of up to 45,000psi/3,100bar, 100 percent guaranteed scalability of results, lifetime training for new employees and students, CE and cGMP compliance and a one-year factory warranty.

Following is a list of the laboratory homogenizers we offer:

  • Nano DeBEE Laboratory Homogenizer - The smallest in our line of laboratory homogenizers, this cost-effective benchtop unit offers a reliable and consistent process for virtually any laboratory setting. With features including a state-of-the art reservoir for small volumes, an adjustable process pressure gauge, a heat exchanger and a back- pressure gauge, this is a popular choice for an entry-level laboratory homogenizer.
  • Micro DeBEE Laboratory Homogenizer – This air-operated, compact, benchtop unit is suitable for a broad range of applications, including cell disruption, particle size reduction, work with polymers and liposomes and the creation of nano and micro emulsions and dispersions. This model offers a bit more capacity and power than the Nano DeBEE Laboratory Homogenizer.
  • Mini DeBEE Laboratory Homogenizer - This cost-effective unit is suitable for both laboratory and research and development work as well as pilot plant and industrial production. It offers the same features as the Nano and Micro DeBEE Laboratory Homogenizers but with added capacity and power. Other features of this model include pressures up to 45,000 psi, 100 percent guaranteed scalability of results and a programmable logic controller a digital display.

Please contact us today to learn more about our laboratory homogenizers or any other homogenizers in our product line. No matter which model you select, they all meet our high standard of quality and offer features easily configured to meet your application needs and processing goals. 

For more information on cell lysis and the multiple methods that could be what your application needs, download our FREE eBook:

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Rotor-Stator vs. High-Pressure Homogenizers: What You Need to Know

Posted by David Shechter on Mar 2, 2018 2:30:00 PM

high-pressure homogenizerIn a world where there are so many different types of products and so many versions of each, it’s easy to get confused. Sometimes I don’t want to spend five minutes in the toothpaste aisle looking through 35 different varieties of basically the same thing!

Even in the homogenization industry, there are multiple models: ultrasonic, rotor-stator bead mill, high-pressure and others. In this blog, I’ll detail rotor-stator and high-pressure homogenizers and list benefits and challenges of each.

Rotor-Stator Homogenizers

Rotor-stator homogenizers, also referred to as high-shear mixers or colloid mills, are designed to reduce particle size through hydraulic and mechanical shear and cavitation. Originally designed to make dispersions and emulsions, this type of homogenizer works through the rotation of a rotor inside a stator, which creates a suction effect. Mixing occurs when a sample is drawn in through one of the mixing heads while the blades rotate and is then expelled at a high velocity through an opening in the stator. The combination of high rpm from the rotor and exit through tiny slots allows the sample to be mechanically sheared into very small particles.

This type of homogenizer is best utilized for biological (plant and animal) tissue and processes liquid samples in the 0.01 ml-20-liter range. It is most often utilized in the chemical, cosmetic, pharmaceutical and food industries to create suspensions, emulsions, lyosols and other products through solid-liquid suspension, liquid-liquid emulsification and homogenization, particle size reduction, power-liquid dispersion and other applications.

Though high pressure-homogenizers employ high amounts of energy to form pressure differentials to homogenize ingredients, their rotor-stator counterparts manipulate ingredients though a shearing action. They generate minimal heat during operation and are able to homogenize fairly soft tissue. However, working with aerosols and foams can be difficult with this type of homogenizer.

High-pressure Homogenizers

Similar to liquid mixers, high-pressure homogenizers work by forcing cell suspensions through a very narrow channel under pressure. They use force (such as turbulence and cavitation) and high pressure to create a consistent and uniform sample. Primarily utilized for cell lysis or creating emulsions when large volumes are being processed, high-pressure homogenizers can be used for multiple types of bacteria, yeast and mycelia. Following are some of the many benefits high-pressure homogenizers provide:

  • Ability to process large volumes of liquid reproducibly and methodically. 
  • Consistency in composition of products.
  • Controlled turbulent flow and cavitation. 
  • Increased rate of dissolution.
  • Low risk of product contamination. 
  • Uniform particle size reduction.
  • Greater stability of final product.
  • Customizable forces to optimize results.
  • Consistent results in fewer passes.
  • Accommodation of a wide range of sample sizes.  
  • Decreased cost.

As you can see, high-pressure homogenizers offer enterprises a lot of benefits. However, it’s important to note that they can’t handle large particles or high-fiber materials and aren’t the most suitable for high-temperature materials. Plus, they can be large and very heavy.

BEE International: Taking the Confusion Out of Homogenization

BEE International offers homogenizers that are both high-quality and reliable. Our wide variety of laboratory, pilot-plant and industrial scale models can help you achieve high quality applications, and we’re trusted by researchers around the world for our laboratory homogenizers and customer support. Contact us today to learn more.

For more information on what you need to know about homogenization and how to achieve efficient and consistent results, download our FREE eBook:

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Top Tips for Selecting a Tissue Homogenizer

Posted by David Shechter on Feb 27, 2018 2:30:00 PM

tissue homogenizerThe first thing many consumers point to when they hear the term “homogenization” is milk. August Gaulin received a patent for his homogenizer in 1899 and exhibited it to “treat” milk in 1900 at the World’s Fair in Paris.

Homogenization works by forcing the sample through a narrow space, and multiple forces  –– including turbulence and cavitation in addition to high pressure –– can act on the sample to create a high quality product. It can be used on many different types of material, such as plant, food, soil, tissue and more. In this blog, we’ll focus on tissue homogenization and what to consider when selecting a homogenizer to fit your specific business needs. 

In summary, tissue homogenization is a process employed to prepare tissue samples for further study, research or development. It involves the lysing (breaking apart) of cells to release their contents, from proteins and small molecules to DNA and RNA and more. The type of cell being lysed often dictates the homogenization device or technique that is used to complete the task. Homogenization is not only usually the simplest and safest approach to preparing such contents, it also is one of the quickest and most cost-effective methods. 

Techniques for Tissue Homogenization 

Knowing what techniques are best utilized for tissue homogenization can make choosing the right product a much easier proposition. These four techniques include chemical homogenization, freeze-thawing, and mechanical and ultrasonic homogenization. 

Chemical homogenization is best for small samples because the cost of materials used can be high for industrial-sized products. Freeze-thawing requires multiple cycles and a lot of time, and ultrasonic homogenization is only appropriate for tissues and molecules that aren’t affected by the temperature increase resulting from the high amount of heat it generates. The mechanical homogenization method can easily be scaled and offers time-efficient and consistent results. Overall, mechanical and ultrasonic homogenization are the two most commonly-used techniques.

High pressure homogenization allows for the forces of turbulence, cavitation, shear and impact to be used simultaneously to produce the best end result, even with delicate tissues. However, the lysate can be of higher quality and more even consistency when run through top-shelf equipment. 

Following is a list of things to consider when selecting a tissue homogenizer:

  • Toughness of tissue - Not all homogenizers can process more fibrous tissue, and those that can typically require a lengthy processing time.
  • Sample size and type – Large samples usually take more time to homogenize. Animal and plant tissues, yeast and bacteria typically require more rigorous methods of disruption.
  • Available components – Consider the product’s motor size, speed control and range, weight and dimensions and processing range.
  • Sample safety – The homogenizer used should be able to rapidly release the protein from its intracellular compartment into a buffer that isn’t harmful to the biological activity of the protein of interest. 
  • Uniformity of samples – The selected homogenizer should have the capability to quickly and safely produce consistent samples.


Before making a final decision on a homogenizer purchase, it’s a good idea to consult with businesses and/or laboratories with needs similar to yours. Another good idea is to request a demonstration from a couple manufacturers and compare the speed, cost and automation capabilities of the products

BEE International: The Top Choice for Tissue Homogenization

BEE International offers a number of high quality, high pressure homogenizers to achieve your goals. With our homogenizers, you will reap the benefits of our process, which includes:

  • Tighter distribution of smaller particles
  • Maximum particle size reduction in fewer passes
  • Increased manufacturing efficiency and reduced cost
To learn more about our line of homogenizers and how we can help you achieve your business goals, please contact us today. If you're looking for more information on how to lyse tissue cells, download our FREE eBook:

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