What Are Homogenizing Agents & How Do They Improve the Homogenization Process?

Posted by Deb Shechter on Dec 17, 2018 12:10:59 PM

homogenizing agentsFor most people, “homogenization” and “homogenizing agents” aren’t words that just roll off the tongue. But for us here at BEE International, talking about them comes as easily as discussing our favorite TV show or the day’s weather. So, if you’ve ever wondered what “homogenized milk” meant or how your face cream was made or why some peanut butter doesn’t have to be stirred, we’d love to share with you the facts about high pressure homogenization and homogenizing agents. Consider the following:

An Overview of the Homogenization Process

Homogenization is the process of combining two substances by breaking them into uniform pieces and mixing them together evenly. Homogenization facilitates many laboratory and manufacturing processes, such as those in the pharmaceutical, biotech, chemical, cosmetic and food industries. Not only does homogenization change the physical characteristics of materials, making it easier for scientists and manufacturers to work with raw components, it also helps ensure product quality, maintain long-term storage of materials and isolate certain particles when necessary. All of these things are necessary for superior performance among a variety of products. Homogenization makes our milk taste good and keeps our peanut butter from separating into a lump of peanut paste and a pool of oil. It keeps our lotions thick, yet spreadable, and our medicines effective.

But what if certain materials resist homogenization attempts by mechanical equipment alone? What if they need something more to help break them apart and realign their parts equally? In these cases, homogenizing agents are used.

What are Homogenizing Agents?

Homogenizing agents are resin-based mixtures which are usually added at the beginning of a homogenization cycle. When heated to certain temperatures, they turn “sticky” and help to moisten and soften the surfaces of the substances being melded together. 

How Do They Improve the Homogenization Process?

Because of their tackiness, homogenizing agents make it easier for some substances to blend and distribute their particles evenly. They also encourage filler acceptance. And by helping to shorten the time it takes to blend materials regardless of their “molecular weight, viscosity or polarity,” homogenizing agents save energy and other valuable resources.

In scientific and research settings, efficient homogenization can do more than just save time, though. It can also save lives. The right homogenizer and the right homogenizing agents can reduce the number of passes it takes to get viable results, ensuring that all steps in the testing cycle are consistent and go as smoothly and quickly as possible.

In more industrial settings, the right homogenizers and homogenizing agents ensure that applications are customized according to overall purposes, whether that be securing product stability; guaranteeing sterility; improving color, texture, taste and nutritional value; or simply maintaining cost effectiveness. 

Need More Info?

If you need help selecting the right homogenizer for your homogenization process, contact us today. Our high pressure homogenizers are customized to suit your own unique needs with multiple mechanical forces that create thoroughly mixed products.

And for additional information, you might also like to download our FREE eBook: "How to Achieve Efficient & Consistent Particle Size Reduction." In it, we show how equipment that reduces particle size saves you money by extending shelf life and strengthening biophysical properties. 

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The Ultimate Cheat Sheet for Choosing a Cell Homogenizer

Posted by David Shechter on Aug 16, 2018 11:30:00 AM

Businessman is standing in front of two direction signs.If you attended high school before laptops and tablet computers replaced books and used a landline to make phone calls, you’ve probably heard of CliffsNotes. Often used to help students figure out the intricacies of a novel that was required reading for English class, these handy pamphlets were a sort of a study guide highlighting the work’s key points.

In this blog, we’re offering you a CliffNotes version of tips for selecting a cell homogenizer that best meets your business needs. We’ll outline cell homogenization, often referred to as cell lysis, offer key factors for how best to achieve it and provide some benefits on it.

What is Cell Lysis?

Cell lysis, which takes place in biotech, pharmaceutical, food, cosmetic and chemical laboratories on a regular basis, is the rupture of the cell membrane resulting in the release of cell contents and the subsequent death of the cell. Research scientists and laboratory technicians disrupt the cell’s structure to extract organelles, proteins, DNA or mRNA. 

Cell lysis can be achieved through mechanical or chemical methods. Mechanical cell lysis techniques include high shear mixing, traditional homogenization, sonication, grinding, freeze/thaw cycles and high-pressure homogenization. Chemical cell lysis techniques include osmotic lysis and the use of detergents, chelating agents or chaotropic agents.

In order for cell lysis to be performed successfully, the following factors should be present:

  • Intracellular proteins should be accessible for extraction and solubilization.
  • The process should be flexible, as different cells require different cell lysis strategies.
  • The technique should result in a high yield in a short time.
  • The process should be relatively easy to perform.
  • Results should be consistently reproducible and scalable.

 

How Homogenization Helps

Of the methods used to achieve cell lysis, the most widely used is homogenization, which offers a multitude of benefits for production laboratories and the consumers who buy their products. It is especially useful for small volumes of cells and/or cultured ones and involves the use of shearing force on the cell. By forcing a small cell through an even smaller-sized orifice, homogenization removes the outer layer and lyses the cell. Some cell lysis products use utilize shear, and others employ sonic cavitation. However, the best choice when purchasing a homogenizer for cell lysis is one that uses shear, cavitation and impact and lets the user adjust the relative amount of each force for different applications.

The homogenizer you choose for cell lysis should be scalable to allow for customization in pressure and intensity, resulting in both cost and time efficiency for the user. It also must accommodate for multiple cell types and adjust for various sample sizes. Other factors to consider when selecting the right cell homogenizer are the force required to produce the highest yield in the least amount of time and the volume, efficiency, stability and sanitary standards compliance of the product. Advantages of using this type of homogenizer are:

  • Accessibility of intracellular proteins.
  • Built-in process flexibility.
  • Ease-of-use and low learning curve.
  • Reproducible and scalable results.
  • Various sample size processing.
  • Ability to disrupt all cell types.
  • Better results in fewer passes.

BEEI International: Bringing the Best Homogenizers to You

BEE International offers numerous high-quality homogenizers to meet the needs of any industry for cell lysis. Our laboratory-grade models include the Nano DeBEEMicro DeBEE and Mini DeBEE, all of which are easy to use, provide consistent and reliable results and easily scale up for full scale production. Plus, they offer maximum experimentation capabilities and the ability to change flow, pressure, shear and impact settings. Contact us today to learn more about our full line of homogenizers.

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Summer, Sunscreen, and Homogenization

Posted by David Shechter on Jun 13, 2018 11:30:00 AM

bee1For many people across the United States, the winter of 2017 was one of the coldest on record. Residents of the East Coast had to deal with multiple Nor'easters, shutting down airports and wreaking havoc on roadways.

For most of us, summer and the high temperatures that typically accompany it are definitely welcome. Kids are out of school, baseball games are being played and swimming pools are being opened and prepped for lazy summer days.

With the arrival of summer, though, comes a reminder about the dangers of getting too much sun. More people are diagnosed with skin cancer annually in the U.S. than all other cancers combined, and the annual cost for treating skin cancers in this country is an estimated $8.1 billion.

Skin cancer may be prevented or at least minimized through the regular use of sunscreen. In fact, scientific evidence supports the benefits of using sunscreen to reduce short- and long-term damage to the skin from the sun’s rays.  

High-pressure homogenization is a preferred method for improving the production of cosmetic creams or lotions, including sunscreen, because it offers the following benefits:

Potent Particle Size Reduction

By forcing a sample through a narrow space while applying high pressure and multiple mechanical forces, including cavitation, shear, impact and turbulence, high-pressure homogenization more effectively decreases particle size. This reduction, or micronization, creates a more homogenous mixture, provides better active ingredient dispersion and stabilizes product emulsions. It’s especially beneficial for creams, lotions and other cosmetic products because of the positive effect on the quality of these items. 

Enriched Texture and Consistency

High-pressure homogenization is often preferred over other methods because of it’s capability to create a product that is uniformly mixed. This leads to smoother texture and enhanced consistency, which is essential for beauty creams and moisturizing lotions because they have to have a combination of the right texture and consistency in order for the skin to be able to effectively absorb them. 

Strengthened Emulsion Stability

Semi-solid products such as lotions and creams are comprised of emulsions, which are a mixture of two immiscible liquids. Micronization of droplets acts to eliminate the separation of water phase of homogenization, resulting in a more permanent mixture of oil and water. This permanence extends the shelf life of these semi-solid products and improves their appearance through consistent coloring.

BEEI: Homogenizers for High-quality Creams and Lotions

If you’re searching for a cosmetic homogenizer to meet your laboratory and/or production needs, make sure you choose one that’s high quality and includes a combination of capabilities. Whether your product is a cream, lotion, makeup or personal care item, opting for a customizable high-pressure homogenizer will only improve and enhance its effectiveness.

Contact us today to learn more about our full line of homogenizers and what they can do for you.

For more information on homogenization and how to achieve efficient and consistent particle size reduction, download our FREE eBook:

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Homogenization and the Industries That Use It

Posted by Tal Shechter on May 29, 2018 2:00:00 PM

bee2Many people go to a local store to buy food, personal care products, medicine and other items. However, most of us don’t spend any time considering what went into the production of these items. In many cases, it likely involved homogenization.

Homogenization is a process utilized for particle size reduction. It works by forcing a sample through a narrow space, and multiple forces, including turbulence and cavitation in addition to high pressure, can act on the sample to create a high-quality product. It can be used on many different types of material, such as plant, food, soil, tissue and more. Plus, it provides benefits for multiple applications, such as high-pressure pasteurization, dispersions, micro/nano emulsions and cell disruption.

Although many other types mixing machines exist, homogenizers are scalable, and their use of multiple mechanical forces instead of just one results in stable, uniform and consistent products. They are used in a wide variety of research and scientific uses and by many industries that require the use of active ingredients because they reduce active ingredients to a small enough size to ensure a thorough dispersion throughout the medium produced.

Pharmaceutical

Powerful mixing and particle size reduction are key techniques among pharmaceutical products. In the pharmaceutical industry, researchers use homogenizers during laboratory research and in clinical trials and manufacturing for products including vaccines, antibiotics, cancer treatment and medicinal creams. Benefits of homogenization for pharmaceutical products include improve delivery of active pharmaceutical ingredients, increased digestibility, lengthened shelf life and improved reliability in manufacturing settings.

The most important uses for homogenizers in the pharmaceutical industry are particle size reduction, scaling for drug development and synthesis of essential drug components. Though other mixing methods exist, homogenization is the most scalable, resulting in increased cost savings, lower time requirements and a decrease in the likelihood of failure for the product for which its used.

Food and Beverage

Many people identify the use of homogenization in the food and beverage industry with milk; it gives milk a longer shelf life and makes it more stable and visually pleasing. Homogenization is primarily used in the food and beverage industry to help manufacturers improve the composition and quality of their products and ensure their end products meet standards for visual appearance, texture and taste. 

Because homogenization utilizes pressure instead of heat, valuable nutrient content and flavor are preserved, chemical structures are stabilized and food processing outcomes are improved. It is a preferred method for producing emulsions, suspensions and other products that require a liquid suspended in another liquid or a small particle size. These products include mayonnaise, ice cream, cream liqueurs, salad dressings, dairy products and more.

Biotech

The biotech industry is comprised of the fields of cellular biology and molecular biology. Homogenizers are most commonly used in this industry to achieve effective cell rupture, enhanced particle size reduction and nanoparticle production. Although they decrease particle size, they ensure that a composition is equal throughout without compromising the overall molecular makeup of the sample.

Homogenizers also can be used to create cost effective medicines, modify microorganisms, synthesize new chemicals and develop alternative biofuels. In the case of cell disruption, they allow for a variety of cells, including E. coli, yeast, bacteria and delicate animal tissues, to be ruptured to recover the contents of essential cells.

BEE International: First on the Road to Reliability

BEE International offers homogenizers that are both high-quality and reliable. Our line of equipment is suitable for virtually every industry and can work in just about any environment. Contact us today to learn how our products can maximize your homogenization processes.

To learn more about 7 key factors to consider when choosing a cell lysis method, no matter the industry you're in, download our FREE eBook:

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Top Tips for Selecting a Tissue Homogenizer

Posted by David Shechter on Feb 27, 2018 2:30:00 PM

tissue homogenizerThe first thing many consumers point to when they hear the term “homogenization” is milk. August Gaulin received a patent for his homogenizer in 1899 and exhibited it to “treat” milk in 1900 at the World’s Fair in Paris.

Homogenization works by forcing the sample through a narrow space, and multiple forces  –– including turbulence and cavitation in addition to high pressure –– can act on the sample to create a high quality product. It can be used on many different types of material, such as plant, food, soil, tissue and more. In this blog, we’ll focus on tissue homogenization and what to consider when selecting a homogenizer to fit your specific business needs. 

In summary, tissue homogenization is a process employed to prepare tissue samples for further study, research or development. It involves the lysing (breaking apart) of cells to release their contents, from proteins and small molecules to DNA and RNA and more. The type of cell being lysed often dictates the homogenization device or technique that is used to complete the task. Homogenization is not only usually the simplest and safest approach to preparing such contents, it also is one of the quickest and most cost-effective methods. 

Techniques for Tissue Homogenization 

Knowing what techniques are best utilized for tissue homogenization can make choosing the right product a much easier proposition. These four techniques include chemical homogenization, freeze-thawing, and mechanical and ultrasonic homogenization. 

Chemical homogenization is best for small samples because the cost of materials used can be high for industrial-sized products. Freeze-thawing requires multiple cycles and a lot of time, and ultrasonic homogenization is only appropriate for tissues and molecules that aren’t affected by the temperature increase resulting from the high amount of heat it generates. The mechanical homogenization method can easily be scaled and offers time-efficient and consistent results. Overall, mechanical and ultrasonic homogenization are the two most commonly-used techniques.

High pressure homogenization allows for the forces of turbulence, cavitation, shear and impact to be used simultaneously to produce the best end result, even with delicate tissues. However, the lysate can be of higher quality and more even consistency when run through top-shelf equipment. 

Following is a list of things to consider when selecting a tissue homogenizer:

  • Toughness of tissue - Not all homogenizers can process more fibrous tissue, and those that can typically require a lengthy processing time.
  • Sample size and type – Large samples usually take more time to homogenize. Animal and plant tissues, yeast and bacteria typically require more rigorous methods of disruption.
  • Available components – Consider the product’s motor size, speed control and range, weight and dimensions and processing range.
  • Sample safety – The homogenizer used should be able to rapidly release the protein from its intracellular compartment into a buffer that isn’t harmful to the biological activity of the protein of interest. 
  • Uniformity of samples – The selected homogenizer should have the capability to quickly and safely produce consistent samples.


Before making a final decision on a homogenizer purchase, it’s a good idea to consult with businesses and/or laboratories with needs similar to yours. Another good idea is to request a demonstration from a couple manufacturers and compare the speed, cost and automation capabilities of the products

BEE International: The Top Choice for Tissue Homogenization

BEE International offers a number of high quality, high pressure homogenizers to achieve your goals. With our homogenizers, you will reap the benefits of our process, which includes:

  • Tighter distribution of smaller particles
  • Maximum particle size reduction in fewer passes
  • Increased manufacturing efficiency and reduced cost
To learn more about our line of homogenizers and how we can help you achieve your business goals, please contact us today. If you're looking for more information on how to lyse tissue cells, download our FREE eBook:

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How Does RNA Extraction Homogenization Work? 

Posted by Deb Shechter on Jan 25, 2018 11:30:00 AM

RNA extraction homogenizationMany people are familiar with the term “DNA,” which is the hereditary material in humans and almost all other organisms. Officially named deoxyribonucleic acid, it a molecule that carries the genetic instructions used in the growth, development, functioning and reproduction of all known living organisms and many viruses.

Not as well known outside the field of science is RNA or ribonucleic acid. RNA, a type of nucleic acid containing only one strand (DNA is primarily double-stranded), provides the link between the genetic information through protein synthesis. Procuring high-grade RNA, which is accomplished through a process called RNA extraction (or isolation), is the first step for a multitude of molecular techniques often utilized in gene expression including microarray analysis, real-time polymerase chain reaction (PCR), digital PCR, northern analysis and cDNA library construction.

RNA Extraction

Using a powerful chaotropic salt solution, RNA extraction purifies RNA from biological samples by quickly disrupting cells or tissue to release the nucleic acids from the cells. These released RNAs are valuable in providing details on which genes are expressed (used to make products), to what degree the genes are active, how they work to regulate each other via small, regulatory RNAs and which structural RNAs (i.e. rRNAs, mRNAs and tRNAs) are produced.

RNA extraction can be very challenging due to the difficulty of isolating RNA that is intact. This is in large part because of ribonuclease (RNase) enzymes found in cells, enzymes which are copious in the environment and can swiftly degrade RNA. Therefore, it’s vital that it be done cautiously and in a timely manner.

Homogenization – Organic RNA Extraction

The disruption of cell and tissue samples, often achieved by homogenization, is a required part of extracting RNAs and preventing their degradation. In fact, samples aren’t guarded from RNA degradation unless they are entirely homogenized. Although homogenization is especially functional for use with soft tissues, the method used should be based on the sort of cell or tissue. For instance, plant and animal tissues, yeast and bacteria typically require more exacting disruption methods. Either way, the sample or tissue should be kept frozen until it is ready to be homogenized.

Utilizing homogenization for RNA extraction is especially beneficial because it enables processing to be effectively and repetitively processed in only seconds, thereby eliminating heat production. Its force and high pressure produce consistent and uniform samples, and it can process both small and large samples. Plus, homogenizers are scalable and compatible with many sample types, including those with tough cell walls.

BEE International – Offering Simple Solutions for Complex Business Needs

We offer a number of top-of-the-line high-pressure homogenizers to achieve your business goals, and our scalable equipment is easy to use and produces better results in fewer passes. In addition, we’re globally recognized by laboratory managers and researchers for our high-quality products and excellent customer support. Contact us today to discuss your homogenization needs.

For more information on how to achieve efficient and consistent particle size reduction, download our FREE eBook:

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Food Homogenization 101: What You Need to Know

Posted by David Shechter on Dec 13, 2017 11:30:00 AM

food homogenizationThe food and beverage industry continues to expand and diversify, especially as consumers look for more healthful products that fit both their lifestyle and budget. Companies must find ways to create and market products to differentiate them from their many competitors while ensuring their goods have the taste, freshness and nutrients on which customers have come to rely.

A common method food processing and manufacturing companies utilize to efficiently, cost-effectively and safely produce their goods is high-pressure homogenization. It not only reduces particle size through forces including high pressure, shear, turbulence, acceleration and impact but also allows for the stable and consistent production of emulsions, suspensions and other commodities that necessitate a liquid suspended in another liquid.

Though one of the most well-known uses of homogenization in the food and beverage industry is for milk, it is also widely used in the manufacture of sauces, spreads, salad dressings, fruit juices and concentrates, fish and meat paté, sweeteners, baby food, egg product, other dairy products and more. The homogenization pressures used for these products typically range between 20 and 60 MPa, but some homogenizers can reach pressures of up to 400 MPa. This magnitude of pressure leads to microbial and enzyme inactivation, which enhances the safety and shelf life of perishable foods while reducing the thermal effects on the foods’ functional and sensory qualities.

Once a company achieves its ideal product, the product needs to be reproduced on a larger scale without losing the characteristics that make it desirable by customers. If it doesn’t offer the same taste and texture on a regular basis, the manufacturer may not only lose customers and their funds but also obtain a damaged reputation.

Food homogenizers help deliver a product with uniform texture, flavoring and taste through a scalable and cost-effective process that allows for a longer period of freshness than food comprised of larger particles. The smaller the particles, the longer the structure of the product can be maintained. Food homogenizers’ ability to use pressure instead of heat preserves critical nutritional components naturally found in a food, enabling them to be available for consumption.

Although high-pressure homogenization may potentially replace thermal methods of food preparation, it does not effectively produce solid foods or ones formed from large particles. Also, because it uses force instead of thermal action, homogenization does not sterilize foods or beverages, requiring it to be accomplished through a separate process.

BEE International: Help in Finding the Best Food Homogenizer

The key to purchasing the right food homogenizer for your business needs is selecting one that can achieve consistent particle size distribution on a scalable basis. At BEE International, our equipment can engineer particle sizes at or below 100 nm, a benefit favorable for most any product in the food and beverage industry. Our products are trusted by researchers and laboratory managers around the world, and our unparalleled customer service will make finding the right product a breeze for companies of all sizes.

Contact us today to find out more about how BEE International can aid your particle size reduction process. 

For more information on the key factors to consider when choosing a cell lysis method, download our FREE eBook:

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How Do Inline Mixers Work Differently Than High-pressure Homogenizers?

Posted by Tal Shechter on Dec 1, 2017 11:30:00 AM

inline mixersWhen they hear the term “homogenization,” many people think of milk. In fact, one of the first applications of homogenization came in the processing of milk. Derived from the Greek word “homogenos” (homos meaning same and genos meaning kind), it is defined as the process of reducing the particle size of an object and is also known as micronization.

Homogenization works by forcing a sample through a narrow space through multiple mechanical forces, thereby producing a stable and consistent product. Easily scalable, it is used by multiple industries, including pharmaceutical, biotech, cosmetic and food, in order to optimize their finished product.

Under the umbrella of homogenization lie two different methods: inline mixing and high-pressure homogenization. Read on to learn the differences between how the two work and advantages associated with each.

Inline Mixing  

Positioned within the production line and aligned with the flow of product, inline mixers consist of a stationary stator and an inside rotor that turns at high speeds. The rotor–stator device is contained in a casing with an inlet at one end and an outlet at the other, and the rotor is driven through a seal. Mixing occurs when a sample is drawn in through one of the mixing heads while the blades rotate and is then expelled at a high velocity through an opening in the stator. The inline term comes into play around the mode of sample entry into the mixing chamber.

Inline mixers feed and output material in a continuous stream, allowing material to often be perfectly hydrated, emulsified or homogenized in a single pass. They differ from other types of homogenization wherein large amounts of ingredients are mixed in a standalone unit before going through the homogenization process. Designed to handle all types of tasks, including emulsifying, suspending, dispersing, blending and more, inline mixers are suitable and useful for applications where larger quantities are needed for production and continuous processing and homogenization is needed during the manufacturing process. In addition to being faster than their high-pressure counterparts, they allow for a more controlled and continuous mixing environment, improve yields, use less energy and increase capacities.

High-pressure Homogenization

Whereas inline mixers rely on rotor/stator shearing action, high-pressure homogenizers force a sample through a narrow space using force and high pressure, thereby reducing particle size or lysing cells. They consist of a high-pressure pump and a disruption unit which enable a continuous homogenization. The higher the amount of energy applied during the homogenization process, the smaller the particle size or the more complete the cell lysis. The pressure along with the action of multiple forces yields impactful results in only one or two passes. Benefits of high-pressure homogenization include consistency in composition of products, greater stability of final products and decreased cost. Also high-pressure homogenizers allow users to set operating parameters - pressure, temperature, number of passes, valve and impingement design and flow rate – for a particular homogenization.

BEE International: Your Homogenization Equipment Headquarters

At BEE International, we offer a wide selection of homogenizers that meet the needs of manufacturers across multiple industries, including cosmetic, pharmaceutical, biotech and more. Our products can help enhance your production, improve product quality and streamline your operations, all in combination with our unmatched customer service. Contact us today to learn more about our homogenization equipment and find out what product(s) is best suited to your business needs.

For more information on particle size reduction and how to achieve efficient and consistent results, download our FREE eBook:

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4 Techniques in Cell Homogenization

Posted by David Shechter on Jul 6, 2017 11:30:00 AM

Cell HomogenizationCell homogenization, also known as cell micronization or cell fractionation, is the action of reducing the particle size of molecules to facilitate even distribution and emulsification of liquids, creams, or other mediums. This process is extremely common and is used in a number of growing industries: chemical, pharmaceutical, biotech, cosmetic, and food.

In the world of cell homogenization, there are several techniques that are commonly used to achieve this task. All of the methods involve encouraging the cells to lyse, or break apart. Cell homogenization can be achieved through various methods, including mechanical disruption, liquid homogenization, sonication, or manual grinding. Continue reading for a brief overview of each method of cell homogenization.

Mechanical Disruption

Mechanical disruption involves the use of rotating blades. These blades work to grind and disperse cells, and they are most effective at homogenizing tissues such as liver.  Rotor-stator homogenizers are one of the best homogenizing tools used in mechanical disruption and can homogenize samples in the volumes from 0.01 milliliters to up to 20 liters, depending on the type of motor that is used. Sample loss is typically minimal, and small amounts of samples and tissues can easily be homogenized using this method.

Liquid Homogenization

Liquid homogenization is the most widely used cell disruption technique, especially with small volumes and cultured cells. In this method, cells are lysed by the action of being forced through a small space, which acts to shear the cell membranes. There are several types of liquid homogenizers on the market, including Potter-Elvehjem homogenizers, french presses, and the dounce homogenizer.

Sonication

Sonication is a type of physical disruption used to lyse cells. This method uses high frequency sound waves to lyse cells, bacteria, and other types of tissue. The sound waves are delivered via a probe that is immersed in the liquid cell suspension. This method, while common, is often time consuming and is best suited for volumes of less than 100 mL.

Manual Grinding

Manual grinding, while one of the most time consuming methods of cell homogenization, is also the most common. In this method, a mortar and pestle is used to manually grind cells. While not suitable for extremely large volumes, this method is the most effective at breaking apart plant tissue cells.

BEE International offers a wide variety of high pressure homogenizers to meet the needs of virtually any industry. Our technology is well suited for nano emulsions, cell lysis, uniform particle reduction, and other related applications.

Contact us today to learn more about how our line of homogenizers can help with your company’s needs.

Also, be sure to check out our FREE eBook on 7 key factors to consider when choosing a cell lysis method

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Local vs. Systemic Steroids: What's the Difference?

Posted by Tal Shechter on Jun 13, 2017 2:30:00 PM

Local vs. Systemic SteroidsWhen most people think of steroids, images of elite athletes using synthetic hormones to gain a competitive advantage may come to mind. However, steroids – more often referred to as corticosteroids in medical circles – are often used to serve a very different purpose. There are numerous varieties of corticosteroid medications, including cortisone, methylprednisolone, and prednisone.

Steroids work by both reducing inflammation and decreasing immune system activity, and they are commonly used to treat a variety of diseases, including rheumatoid arthritis, eczema, asthma, and a host of other inflammatory ailments.

Steroids come in several different forms – local and systemic.  The form of steroid used is dependent on the ailment that is being treated, as well as the desired outcome.

Local steroid medications are best suited for ailments where a targeted approach is desired. These types of steroids can be introduced directly to the area in question and include eye drops, ear drops, skin creams, and joint injections.

Systemic steroids are best suited for ailments that require treatment throughout the entire body. Systemic steroids circulate throughout the bloodstream and reach every area of the body. They include oral medications as well as medications that are delivered intravenously or intramuscularly.

For both local and systemic applications, homogenization is an important process step to consider in industries that manufacture steroid-containing topical creams, ointments, and intravenous drugs.

In all applications, the even dispersion of active ingredients is critical to ensuring efficacy and decreasing manufacturing costs. Oral medications, for example, require homogenization and micronization of particles to ensure consistency throughout the product. Similarly, systemic steroids require the use of a homogenizer to ensure even dispersion and dissolution of the particles that make up the active ingredients.

BEE International offers a number of homogenizers that meet the needs of manufacturers across multiple industries, including cosmetic, pharmaceutical, and biotech. Our homogenizers can meet the needs of virtually any environment – from small R&D settings and pilot plant operations all the way to 24/7 manufacturing environments.

For small sample sizes, as are often seen in laboratory and R&D settings, our Nano DeBEE is an excellent choice. This mechanical homogenizer uses multiple forces, including cavitation, turbulence, and pressure to create microscopic particles that can easily be dissolved in a variety of mediums, including liquids, creams, and organic solvents.

Contact us today to discuss the numerous benefits our homogenizers can offer to manufacturing your company's products. 

Also, be sure to check out our FREE eBook on how to achieve efficient and consistent particle size reduction

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