What Are Homogenizing Agents & How Do They Improve the Homogenization Process?

Posted by Deb Shechter on Dec 17, 2018 12:10:59 PM

homogenizing agentsFor most people, “homogenization” and “homogenizing agents” aren’t words that just roll off the tongue. But for us here at BEE International, talking about them comes as easily as discussing our favorite TV show or the day’s weather. So, if you’ve ever wondered what “homogenized milk” meant or how your face cream was made or why some peanut butter doesn’t have to be stirred, we’d love to share with you the facts about high pressure homogenization and homogenizing agents. Consider the following:

An Overview of the Homogenization Process

Homogenization is the process of combining two substances by breaking them into uniform pieces and mixing them together evenly. Homogenization facilitates many laboratory and manufacturing processes, such as those in the pharmaceutical, biotech, chemical, cosmetic and food industries. Not only does homogenization change the physical characteristics of materials, making it easier for scientists and manufacturers to work with raw components, it also helps ensure product quality, maintain long-term storage of materials and isolate certain particles when necessary. All of these things are necessary for superior performance among a variety of products. Homogenization makes our milk taste good and keeps our peanut butter from separating into a lump of peanut paste and a pool of oil. It keeps our lotions thick, yet spreadable, and our medicines effective.

But what if certain materials resist homogenization attempts by mechanical equipment alone? What if they need something more to help break them apart and realign their parts equally? In these cases, homogenizing agents are used.

What are Homogenizing Agents?

Homogenizing agents are resin-based mixtures which are usually added at the beginning of a homogenization cycle. When heated to certain temperatures, they turn “sticky” and help to moisten and soften the surfaces of the substances being melded together. 

How Do They Improve the Homogenization Process?

Because of their tackiness, homogenizing agents make it easier for some substances to blend and distribute their particles evenly. They also encourage filler acceptance. And by helping to shorten the time it takes to blend materials regardless of their “molecular weight, viscosity or polarity,” homogenizing agents save energy and other valuable resources.

In scientific and research settings, efficient homogenization can do more than just save time, though. It can also save lives. The right homogenizer and the right homogenizing agents can reduce the number of passes it takes to get viable results, ensuring that all steps in the testing cycle are consistent and go as smoothly and quickly as possible.

In more industrial settings, the right homogenizers and homogenizing agents ensure that applications are customized according to overall purposes, whether that be securing product stability; guaranteeing sterility; improving color, texture, taste and nutritional value; or simply maintaining cost effectiveness. 

Need More Info?

If you need help selecting the right homogenizer for your homogenization process, contact us today. Our high pressure homogenizers are customized to suit your own unique needs with multiple mechanical forces that create thoroughly mixed products.

And for additional information, you might also like to download our FREE eBook: "How to Achieve Efficient & Consistent Particle Size Reduction." In it, we show how equipment that reduces particle size saves you money by extending shelf life and strengthening biophysical properties. 

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Why We Love High Pressure Homogenizers (And You Should, Too!)

Posted by David Shechter on Aug 30, 2018 11:30:00 AM

Love High Pressure HomogenizersIf you were asked about some of your favorite products, a high-pressure homogenizer may not make the list. However, as we’ve discussed in a previous blog, the homogenization process produces a lot of products you most likely use and consume in your daily life. That’s one of the reasons we here at BEE International love homogenizers…but there are more! Let’s take a look at some of the most popular uses of high-pressure homogenization and the benefits they offer consumers.

Particle Size Reduction

High-pressure homogenization utilizes mechanical force to break down particles or droplets in a liquid into smaller and more uniform sizes, resulting in a dispersion, which is where fine particles of one substance are scattered throughout another substance. Dispersions can be further classified into solutions, suspensions and colloids, a mixture somewhere between a solution and a suspension.

Some high-pressure homogenizers can reduce particle size to as small as a single-digit nanometer. Plus, they ensure the composition is equal throughout, and the overall molecular makeup of the sample isn’t compromised. Many high-pressure homogenizers are able to produce emulsions, dispersions and suspensions at particle sizes of 100 µm or higher, and a select few can achieve a size of down to 0.1 µm. Key benefits of particle size reduction include:

  • Increased dissolution rate.

  • Improved drug delivery.

  • Controlled rate of reaction.

  • Increased density.

  • Consistent texture and product appearance.

  • Enhanced sedimentation stability.

  • Improved mineral processing.


Important Applications

Food and Beverages

Though one of the most well-known uses of high-pressure homogenization in the food and beverage industry is for milk, it is also widely used in the manufacture of sauces, spreads, salad dressings, fruit juices and concentrates, fish and meat pâté, sweeteners, baby food, egg product, other dairy products and more. It helps companies utilize to efficiently, cost-effectively and safely produce their goods. Food homogenizers also aid in the delivery of a product with uniform texture, flavoring and taste through a scalable process that allows for a longer period of freshness than food comprised of larger particles.

Pharmaceuticals, Chemicals and Biofuels

High-pressure homogenizers are used for numerous products from the chemical and biotechnology industries and products such as pharmaceutical tablet coatings, vaccinations and creams. They can be used to create cost effective medicines, modify microorganisms, synthesize new chemicals and develop alternative biofuels.

Cosmetic Creams and Lotions

Due to its ability to provide potent particle size reduction, enriched texture and consistency and strengthened emulsion stability, high-pressure homogenization is a preferred method for improving the production of cosmetic creams, lotions and other cosmetic products. It is especially beneficial for beauty creams and moisturizing lotions because they have to have a combination of the right texture and consistency in order for the skin to be able to effectively absorb them.

RNA Extraction

 Utilizing homogenization for RNA extraction enables processing to be effectively and repetitively processed in only seconds, thereby eliminating heat production. Its force and high pressure produce consistent and uniform samples, and it can process both small and large samples. Plus, homogenizers are scalable and compatible with many sample types, including those with tough cell walls. Using a powerful chaotropic salt solution, RNA extraction purifies RNA from biological samples by quickly disrupting cells or tissue to release the nucleic acids from the cells. These released RNAs are valuable in providing details on which genes are expressed (used to make products), to what degree the genes are active, how they work to regulate each other via small, regulatory RNAs and which structural RNAs (i.e. rRNAs, mRNAs and tRNAs) are produced.

Oil Emulsification

 Due to the fact that water and oil are immiscible, powerful mixing is essential to conducting oil emulsification. Therefore, specialized machines such as high-pressure homogenizers are preferred because they not only impart high shear but also are able to reduce particle sizes much more efficiently than other blending and emulsification methods. They are used in a wide variety of research and scientific uses and by many industries that require the use of active ingredients because they reduce active ingredients to a small enough size to ensure a thorough dispersion throughout the medium produced. Additional benefits of high-pressure homogenization for oil emulsification include production of stable emulsions, improved product consistency, shelf-life, texture, color and flavor and the ability to overcome the resistance caused by two immiscible liquids.

BEE International: Our Love for Our Products Shows in the Way We Treat Our Customers

BEE International offers homogenizers that are both high-quality and reliable. Our line of equipment is suitable for virtually every industry and can work in just about any environment. Contact us today to learn how our products can maximize your homogenization processes.

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The Ultimate Cheat Sheet for Choosing a Cell Homogenizer

Posted by David Shechter on Aug 16, 2018 11:30:00 AM

Businessman is standing in front of two direction signs.If you attended high school before laptops and tablet computers replaced books and used a landline to make phone calls, you’ve probably heard of CliffsNotes. Often used to help students figure out the intricacies of a novel that was required reading for English class, these handy pamphlets were a sort of a study guide highlighting the work’s key points.

In this blog, we’re offering you a CliffNotes version of tips for selecting a cell homogenizer that best meets your business needs. We’ll outline cell homogenization, often referred to as cell lysis, offer key factors for how best to achieve it and provide some benefits on it.

What is Cell Lysis?

Cell lysis, which takes place in biotech, pharmaceutical, food, cosmetic and chemical laboratories on a regular basis, is the rupture of the cell membrane resulting in the release of cell contents and the subsequent death of the cell. Research scientists and laboratory technicians disrupt the cell’s structure to extract organelles, proteins, DNA or mRNA. 

Cell lysis can be achieved through mechanical or chemical methods. Mechanical cell lysis techniques include high shear mixing, traditional homogenization, sonication, grinding, freeze/thaw cycles and high-pressure homogenization. Chemical cell lysis techniques include osmotic lysis and the use of detergents, chelating agents or chaotropic agents.

In order for cell lysis to be performed successfully, the following factors should be present:

  • Intracellular proteins should be accessible for extraction and solubilization.
  • The process should be flexible, as different cells require different cell lysis strategies.
  • The technique should result in a high yield in a short time.
  • The process should be relatively easy to perform.
  • Results should be consistently reproducible and scalable.

 

How Homogenization Helps

Of the methods used to achieve cell lysis, the most widely used is homogenization, which offers a multitude of benefits for production laboratories and the consumers who buy their products. It is especially useful for small volumes of cells and/or cultured ones and involves the use of shearing force on the cell. By forcing a small cell through an even smaller-sized orifice, homogenization removes the outer layer and lyses the cell. Some cell lysis products use utilize shear, and others employ sonic cavitation. However, the best choice when purchasing a homogenizer for cell lysis is one that uses shear, cavitation and impact and lets the user adjust the relative amount of each force for different applications.

The homogenizer you choose for cell lysis should be scalable to allow for customization in pressure and intensity, resulting in both cost and time efficiency for the user. It also must accommodate for multiple cell types and adjust for various sample sizes. Other factors to consider when selecting the right cell homogenizer are the force required to produce the highest yield in the least amount of time and the volume, efficiency, stability and sanitary standards compliance of the product. Advantages of using this type of homogenizer are:

  • Accessibility of intracellular proteins.
  • Built-in process flexibility.
  • Ease-of-use and low learning curve.
  • Reproducible and scalable results.
  • Various sample size processing.
  • Ability to disrupt all cell types.
  • Better results in fewer passes.

BEEI International: Bringing the Best Homogenizers to You

BEE International offers numerous high-quality homogenizers to meet the needs of any industry for cell lysis. Our laboratory-grade models include the Nano DeBEEMicro DeBEE and Mini DeBEE, all of which are easy to use, provide consistent and reliable results and easily scale up for full scale production. Plus, they offer maximum experimentation capabilities and the ability to change flow, pressure, shear and impact settings. Contact us today to learn more about our full line of homogenizers.

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Making the Right Choice: Homogenizer Manufacturers

Posted by David Shechter on Jul 31, 2018 11:30:00 AM

choosing high pressure homogenizerShopping for certain products is easy. If you want a fast food hamburger, you have a few well-known establishments from which to choose. If you’re looking to purchase some products to stain a deck or tend to weeds in your lawn, you most likely go to your local hardware store or one of a couple bigger, nationwide home improvement stores.

Investing in a more expensive product often requires more research and comparison. Usually, you can read customer reviews online or find ratings published by consumer organizations. When purchasing a homogenizer, however, it may be harder for you to obtain the advice needed to make the right decision.

First, let’s do a little refresher on homogenization. The primary methods used for homogenization include ultrasonic, mechanical and high-pressure, and homogenizers are used across multiple industries, such as:

  • Pharmaceutical: vaccines, antibiotics, cancer treatment and medicinal creams.
  • Food and Beverage: mayonnaise, ice cream, cream liqueurs, salad dressings, dairy products and more.
  • Biotech: creation of cost-effective medicines, modification of microorganisms, synthesization of chemicals and development of alternative biofuels.

Based on these details, the first step in finding a homogenizer manufacturer in asking yourself the following questions:

  1. Why do I need one?
  2. What differentiates one homogenizer from similar ones on the market?
  3. Does my product require a specific particle size?
  4. Which accessories are recommended and/or required for optimal performance?
  5. What motor power does my product require?
  6. Do I have a desired tube capacity and/or mixing speed?

By understanding the available options and what can best help you accomplish your specific homogenization goals, you are better equipped to select a manufacturer to meet your specific needs. It’s important to choose a manufacturer who will work with you to customize a homogenizer that delivers the best possible results. Some companies are able to customize the forces in their homogenizers to suit your application. Some even produce separate homogenizers for each phase of drug development.

Consistency is essential in homogenization, so employing the services of a manufacturer who aids you in reproducing reliable results is imperative. They should offer a homogenizer that is easy to scale and enables the product developer to quickly apply a wide variety of mixing techniques to a formulation. The manufacturer should have a reputation for reliability, offer customer referrals and provide you with at least once demonstration of their product(s).

Once you’ve decided on a manufacturer from which to purchase your homogenizer, factors to consider when choosing a brand and model include:

  • Sample types.
  • Maximum operating pressure. 
  • Mixing speed.
  • Processing setup. 
  • Processing range. 

 

BEE International: The Right Choice for the Best Reproducible Results

Our technology achieves superior results for nano emulsions, cell lysis, uniform particle reduction and related applications, and we offer homogenizers that are high-quality, customizable and reliable. Plus, we provide unparalleled customer service from demo to purchase and beyond. Contact us today for more information on our entire line of homogenization equipment.

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Six Tips for Ensuring Safety When Using Your High-Pressure Homogenizer

Posted by Deb Shechter on Jul 12, 2018 10:30:00 AM

high pressure homogenizerSafety first. It’s a phrase you hear fairly often, and although it may seem unnecessary to repeat, it’s especially important when working with large machines or those with many parts. When not used correctly, these machines can not only fail to produce an optimal product but also cause injury. 

Homogenizers fit into this category, so we’re offering the following tips on how to use yours and the samples it produces both safely and effectively:

1. Wear the appropriate protective equipment.

As with any such machine, these elements are designed to keep the user safe from moving parts and any hazardous chemicals. Whether it’s gloves, eye goggles or even ear plugs, investing in these accessories can keep you from damaging any part of your body, especially your eyes and ears. Some high-pressure homogenizers can reach 85 dBA and potentially cause ear damage.

2. Follow operating instructions and regulations.

There are safety laws and guidelines produced by unions and the government for a reason. These rules are designed to keep you safe while ensuring the machine you’re using is working as it was designed to do. To make sure you always have your high-pressure homogenizer’s instruction manual when you need it, keep it with the machine, and store a copy with your other important documents.

3. Use only the parts that came with your high-pressure homogenizer.

The parts that make up your high-pressure homogenizer are designed to work together and should not be substituted with another accessory or attachment. Failure to follow this rule may cause your product to work at a decreased level or even break, often voiding your warranty.

4. Avoid contact with the rotating shaft and homogenizing head.

Contact with either of these sharp parts can cause injury, so make sure you always switch the machine off before cleaning it or changing screens. Be especially careful when handling your high-pressure homogenizer after it has been contaminated with dangerous samples.

5. Let only trained operators handle your high-pressure homogenizer.

When installing or operating your homogenizer or simply doing maintenance on it, have only those trained to do so perform the task. Improper handling can cause both machine malfunction and minor injury to the handler.

6. Follow common electrical precautions.

Again, these rules are in place for a reason, and failing to follow such rules may result in electrocution or shock.

BEE International: Putting Safety First

At BEE International, our line of equipment is suitable for virtually every industry and can work in just about any environment, letting you focus on other important projects. Plus, we work with you to make sure you understand the appropriate safety precautions and provide training for all our products. To learn more, please contact us today.New Call-to-action

Homogenizers and the Forces That Drive Them

Posted by Deb Shechter on Jun 25, 2018 11:30:00 AM

forceForce sounds like a very scientific term, but it basically consists of pushing, pulling and gravity. Pushes and pulls set things in motion, while gravity pulls down everything on earth. The six kinds of forces that act on objects when they come into contact with one another are: normal, applied, frictional, tension, spring and resisting.

In high-pressure homogenization, mechanical forces enhance the effectiveness of utilizing extremely high operating pressures. The combination of four main forces – cavitation, high shear, impact and turbulence – are used with high pressure to achieve particle size reduction, cell lysis and/or effective emulsification.

Cavitation

Cavitation occurs when a high amount of pressure is exerted on a liquid sample. Pressure enables the liquid to achieve high kinetic energy, thereby increasing velocity. Also sometimes described as the formation of liquid-free spaces within a liquid, cavitation is especially useful for particle reduction and can be intensified or reduced by adjusting the size of a high-pressure homogenizer’s nozzle.

Although homogenization can be accomplished without cavitation, it increases the efficiency of the process. In the food industry, cavitation in homogenization breaks up large fat globules to create a stable emulsion for increased shelf life and improve taste and texture.

High Shear

High-pressure homogenizers work through shear force, which can be created when a tangential force is applied to a sample. Homogenization using high shear allows for even further reduction of particle size by mixing two normally immiscible phases, such as with water-in-oil (w/o) or oil-in-water (o/w) emulsions.

Many high shear mixers are used to reduce particle size, but high-pressure homogenizers are able to attain even higher shear rates. This is especially important when a dispersion is required.

Impact

The force of impact works in homogenization to reduce the particle size of solids into liquids and achieve lysis of difficult-to-disrupt cells and other materials though the use of grinding beads. Dounce and Potter-Elvehjem homogenizers use only the force of impact to lyse cells, while the French press counterpart adds in high shear. The ability of high-pressure homogenizers to combine multiple forces offers manufacturers better yield in fewer passes and a lower time requirement. Along with other mechanical forces, impact can be fine-tuned to accommodate a specific sample.

Turbulence

In the overall homogenization process, turbulent premixing is the stage designed to prepare a sample by mechanically loosening bonds. By high-pressure machines like stirrers and agitators putting a sample through turbulent premixing, the homogenization process is easier and more time-efficient.

Another influence in homogenization is process intensity; higher intensity is used to obtain smaller particle sizes in fewer passes. Though not all homogenizers are equipped with adjustable process intensities, those from BEE International allow for increased or reduced process intensity with only the turn of a dial.

BEE International: The Driving Force Behind the Best High-Pressure Homogenizers

Unlike other technologies which apply one mechanical force to mix a product, BEE technology utilizes all available mechanical forces to achieve optimum results. We synergistically combine and fine-tune these forces to produce the best possible product for you and let you produce a tight distribution of small, uniform particles every time. Contact us to learn more!

For more information on key factors to consider when choosing a cell lysis method, download our FREE eBook:

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Pharmaceutical Equipment Manufacturers: Five Questions to Ask Before Purchasing a Homogenizer

Posted by David Shechter on May 21, 2018 11:00:00 AM

pharmaceutical equipment manufacturers homogenizerBuying an automobile or house may be a complex process, but you often want to perform detailed research before making such a substantial purchase. You want to ensure your purchase has the capabilities you require, fits within your budget and offers a good investment.

For manufacturers of pharmaceutical equipment, doing research before buying a homogenizer or any other sizable item necessary for production is key to finding the right one. Though this process many seem time-consuming, you’ll end up with a product that best fits your needs and gives you the most benefits. Following are ten questions to ask before buying a homogenizer:

Why do I need one?

Though homogenizers are valuable tools for manufacturers in multiple industries, different models typically address different needs. For instance, maybe you need a homogenizer to mass-manufacture a product that has been approved for market. Or, perhaps you’re conducting research and development and need to disrupt yeast cells. At BEE International, we produce separate homogenizers for each phase of drug development. Make sure the product you choose is best suited to your specific business needs.

What differentiates one homogenizer from similar ones on the market?

In your research for a homogenizer, be sure to ask each manufacturer about their product’s specific capabilities and benefits. Their customer support personnel should be able to articulate any unique functions and/or specifications their products have and define ways it may be more cost-effective than competing products.

Does my product require a specific particle size?

To purchase a homogenizer which provides maximum laboratory output, you should ensure you know the required consistency in texture and size for your product to achieve safety and efficacy. Most pharmaceutical products, including vaccines, inhalants and anesthetics, need to be synthesized using a specific particle size. Many high-pressure homogenizers are able to produce emulsions, dispersions and suspensions at particle sizes of 100 µm or higher, but only a select few can achieve a size of down to 0.1 µm.

Which accessories are recommended and/or required for optimal performance?

Many times, a homogenizer without a lot of bells and whistles will perform the job for which you selected it well. However, some accessories are designed to offer benefits such as time savings, improved process accuracy and enhanced process efficiency. For example, deflector heads can facilitate homogenization of viscous material, generator probes can prevent cross-contamination and pre-assembled bead kits can make the process much easier. Check with each manufacturer to see if any specific accessories are needed for their homogenizing product to run at its highest possible level.

Do you have a demo machine we can test to see if your product meets our needs?

Before you purchase any homogenizer from any manufacturer, it’s important to try each one out to make sure it’s the best fit for your laboratory. For instance, if you handle high volumes of samples, you should try a homogenizer with a high motor capacity for optimal processing efficiency. If you have a desired mixing speed, you should examine homogenizers that let you easily achieve and maintain this speed or change it as necessary.

Other questions to ask when looking to purchase a homogenizer should center around company reputation, product warranties, level of customer service after purchase, user training and education, output capacity and ease of use. Taking the time to select the homogenizer best suited to your needs will be worth the effort.

BEE International: Trust Us with Your Homogenization Needs

At Bee International, we have extensive experience in the challenges our pharmaceutical customers face as they transition from concept, R&D, clinical trials and all-important FDA approval to manufacturing. Plus, we’re trusted by pharmaceutical researchers and laboratory managers around the world. Contact us today to learn more about our complete line of homogenizers.

For more information on the advantages of BEE homogenizers for the pharmaceutical industry, download our FREE eBook:

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High-pressure Homogenizers: Enhancing Oil Emulsification

Posted by Deb Shechter on Mar 30, 2018 10:30:00 AM

high-pressure homogenizersYou might not think your daily life involves emulsions unless you work in a scientific field. However, emulsions and the products of which they are a part are found in a variety of industries, from pharmaceuticals and food manufacturing to metal processing and more. For example, milk and butter are emulsions found in daily life, and emulsions are responsible for enhancing both the textural and visual properties of medicated creams.

So what exactly is an emulsion? Basically, it’s a mixture of oily and watery liquids. There are two primary kinds of emulsions: oil suspended in water (o/w) and water suspended in oil (w/o). Both are inherently unstable and require force and functional chemicals, otherwise known as emulsifiers, to break apart oil droplets in order to mix with watery liquid. Following are three main differences between o/w and w/o emulsions: 

Suspended vs. continuous phase

Probably the most basic yet significant difference between o/w and w/o emulsions is which phase is suspended and which is continuous. Oil and water are normally immiscible, but a permanent mixture or emulsion can be achieved with the use of proper mixing and stability agents. In this case, smaller droplet sizes improve the effectiveness of either system, which may translate to increased bioavailability in pharmaceutical products or extended shelf-life in food/beverage products.

Type of product that can be created

O/w emulsions are the basis for water-based products; in the pharmaceutical industry, they can be found in creams like moisturizers and topical steroid products. W/o emulsions, though, form oil-based products such as sunscreen and many types of makeup.

Method used to achieve stability

All emulsions, whether w/o or o/w, require an emulsifier to assist with stability. O/w emulsions typically require more than one emulsifier, and they can be acquired separately or in a pre-mixed cocktail. In contrast, while w/o emulsions need one emulsifier, there are a limited number from which to select because the hydrophilic balance must be in a narrow range (3-6).

Again, due to the fact that water and oil are immiscible, powerful mixing is essential to conducting oil emulsification. Therefore, specialized machines such as high-pressure homogenizers, which work by forcing a sample through a narrow space and employing multiple forces, including turbulence and cavitation in addition to high pressure, are preferred. They not only impart high shear but also are able to reduce particle sizes much more efficiently than other blending and emulsification methods. In fact, high-pressure homogenizers can reduce droplet size to under one µm, produce more consistent emulsions and reduce creaming rate, thereby boosting the shelf-life of the emulsions.

Additional benefits of high-pressure homogenization for oil emulsification include production of stable emulsions, improved product consistency, shelf-life, texture, color and flavor and the ability to overcome the resistance caused by two immiscible liquids. Also, most high-pressure homogenizers currently manufactured offer reduced maintenance and reduced vibration and noise.

BEE International: Bringing the Benefits of Homogenization Straight to You

We know there are other providers of homogenization equipment, but at BEE International, our expertise, industry experience and level of customer service place us above the rest. Whatever your business needs, we have the product(s) to meet and exceed them. For more information, please contact us today.

For more information on how to achieve efficient and consistent results for your application, check out our FREE animation:

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Rotor-Stator vs. High-Pressure Homogenizers: What You Need to Know

Posted by David Shechter on Mar 2, 2018 2:30:00 PM

high-pressure homogenizerIn a world where there are so many different types of products and so many versions of each, it’s easy to get confused. Sometimes I don’t want to spend five minutes in the toothpaste aisle looking through 35 different varieties of basically the same thing!

Even in the homogenization industry, there are multiple models: ultrasonic, rotor-stator bead mill, high-pressure and others. In this blog, I’ll detail rotor-stator and high-pressure homogenizers and list benefits and challenges of each.

Rotor-Stator Homogenizers

Rotor-stator homogenizers, also referred to as high-shear mixers or colloid mills, are designed to reduce particle size through hydraulic and mechanical shear and cavitation. Originally designed to make dispersions and emulsions, this type of homogenizer works through the rotation of a rotor inside a stator, which creates a suction effect. Mixing occurs when a sample is drawn in through one of the mixing heads while the blades rotate and is then expelled at a high velocity through an opening in the stator. The combination of high rpm from the rotor and exit through tiny slots allows the sample to be mechanically sheared into very small particles.

This type of homogenizer is best utilized for biological (plant and animal) tissue and processes liquid samples in the 0.01 ml-20-liter range. It is most often utilized in the chemical, cosmetic, pharmaceutical and food industries to create suspensions, emulsions, lyosols and other products through solid-liquid suspension, liquid-liquid emulsification and homogenization, particle size reduction, power-liquid dispersion and other applications.

Though high pressure-homogenizers employ high amounts of energy to form pressure differentials to homogenize ingredients, their rotor-stator counterparts manipulate ingredients though a shearing action. They generate minimal heat during operation and are able to homogenize fairly soft tissue. However, working with aerosols and foams can be difficult with this type of homogenizer.

High-pressure Homogenizers

Similar to liquid mixers, high-pressure homogenizers work by forcing cell suspensions through a very narrow channel under pressure. They use force (such as turbulence and cavitation) and high pressure to create a consistent and uniform sample. Primarily utilized for cell lysis or creating emulsions when large volumes are being processed, high-pressure homogenizers can be used for multiple types of bacteria, yeast and mycelia. Following are some of the many benefits high-pressure homogenizers provide:

  • Ability to process large volumes of liquid reproducibly and methodically. 
  • Consistency in composition of products.
  • Controlled turbulent flow and cavitation. 
  • Increased rate of dissolution.
  • Low risk of product contamination. 
  • Uniform particle size reduction.
  • Greater stability of final product.
  • Customizable forces to optimize results.
  • Consistent results in fewer passes.
  • Accommodation of a wide range of sample sizes.  
  • Decreased cost.

As you can see, high-pressure homogenizers offer enterprises a lot of benefits. However, it’s important to note that they can’t handle large particles or high-fiber materials and aren’t the most suitable for high-temperature materials. Plus, they can be large and very heavy.

BEE International: Taking the Confusion Out of Homogenization

BEE International offers homogenizers that are both high-quality and reliable. Our wide variety of laboratory, pilot-plant and industrial scale models can help you achieve high quality applications, and we’re trusted by researchers around the world for our laboratory homogenizers and customer support. Contact us today to learn more.

For more information on what you need to know about homogenization and how to achieve efficient and consistent results, download our FREE eBook:

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Top Tips for Selecting a Tissue Homogenizer

Posted by David Shechter on Feb 27, 2018 2:30:00 PM

tissue homogenizerThe first thing many consumers point to when they hear the term “homogenization” is milk. August Gaulin received a patent for his homogenizer in 1899 and exhibited it to “treat” milk in 1900 at the World’s Fair in Paris.

Homogenization works by forcing the sample through a narrow space, and multiple forces  –– including turbulence and cavitation in addition to high pressure –– can act on the sample to create a high quality product. It can be used on many different types of material, such as plant, food, soil, tissue and more. In this blog, we’ll focus on tissue homogenization and what to consider when selecting a homogenizer to fit your specific business needs. 

In summary, tissue homogenization is a process employed to prepare tissue samples for further study, research or development. It involves the lysing (breaking apart) of cells to release their contents, from proteins and small molecules to DNA and RNA and more. The type of cell being lysed often dictates the homogenization device or technique that is used to complete the task. Homogenization is not only usually the simplest and safest approach to preparing such contents, it also is one of the quickest and most cost-effective methods. 

Techniques for Tissue Homogenization 

Knowing what techniques are best utilized for tissue homogenization can make choosing the right product a much easier proposition. These four techniques include chemical homogenization, freeze-thawing, and mechanical and ultrasonic homogenization. 

Chemical homogenization is best for small samples because the cost of materials used can be high for industrial-sized products. Freeze-thawing requires multiple cycles and a lot of time, and ultrasonic homogenization is only appropriate for tissues and molecules that aren’t affected by the temperature increase resulting from the high amount of heat it generates. The mechanical homogenization method can easily be scaled and offers time-efficient and consistent results. Overall, mechanical and ultrasonic homogenization are the two most commonly-used techniques.

High pressure homogenization allows for the forces of turbulence, cavitation, shear and impact to be used simultaneously to produce the best end result, even with delicate tissues. However, the lysate can be of higher quality and more even consistency when run through top-shelf equipment. 

Following is a list of things to consider when selecting a tissue homogenizer:

  • Toughness of tissue - Not all homogenizers can process more fibrous tissue, and those that can typically require a lengthy processing time.
  • Sample size and type – Large samples usually take more time to homogenize. Animal and plant tissues, yeast and bacteria typically require more rigorous methods of disruption.
  • Available components – Consider the product’s motor size, speed control and range, weight and dimensions and processing range.
  • Sample safety – The homogenizer used should be able to rapidly release the protein from its intracellular compartment into a buffer that isn’t harmful to the biological activity of the protein of interest. 
  • Uniformity of samples – The selected homogenizer should have the capability to quickly and safely produce consistent samples.


Before making a final decision on a homogenizer purchase, it’s a good idea to consult with businesses and/or laboratories with needs similar to yours. Another good idea is to request a demonstration from a couple manufacturers and compare the speed, cost and automation capabilities of the products

BEE International: The Top Choice for Tissue Homogenization

BEE International offers a number of high quality, high pressure homogenizers to achieve your goals. With our homogenizers, you will reap the benefits of our process, which includes:

  • Tighter distribution of smaller particles
  • Maximum particle size reduction in fewer passes
  • Increased manufacturing efficiency and reduced cost
To learn more about our line of homogenizers and how we can help you achieve your business goals, please contact us today. If you're looking for more information on how to lyse tissue cells, download our FREE eBook:

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