David Shechter

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What Does a Homogenizer Do?

Posted by David Shechter on Jul 16, 2019 9:25:31 AM

What Does a Homogenizer Do?There are many types of homogenizers, from the basic kitchen blender and handheld, laboratory dounce homogenizer that use mechanical force to break apart and blend food or other “ingredients” to the numerous kinds of large, industrial homogenizers that rely on pressure to effectively disrupt and mix microscopic components of a sample. There are even ultrasonic homogenizers that use cavitation to produce similar results. But just what does a homogenizer do, you might wonder? How does it work?

 

While the specifics of how a homogenizer does what it does changes based on the composition of the materials on hand and the proposed intent of the product to be made, the basic premise of what it does is always the same. Thus, despite the fact that there are dozens of different types of homogenizers, all of them basically do the same thing: apply force(s) in order to separate the parts of one substance and thoroughly mix them with the parts of another.

 

Why Use Homogenizers?

Homogenizers are used to completely mix a solid, liquid or gas into another substance, but they also offer several other key benefits and, thus, are invaluable across numerous industrial, chemical, biotechnological and food manufacturing processes. Specifically, in addition to the aforementioned blending purpose, businesses use homogenizers to reduce the particle size of various types of substances; facilitate the elimination of pathogens in food products (also known as pasteurization); improve the stability, appearance and texture of chemical compounds (including things such as paint, makeup and medications); increase the bioavailability of pharmaceutical products; extend product shelf life; maintain consistent levels of quality; and reduce manufacturing costs.

 

In short, homogenizers enable companies to guarantee efficacy and efficiency no matter their application. Indeed, some homogenizers achieve results by agitating and stirring. Some do it by crushing and grinding. Others push suspensions through narrow passages, and some use pressure and impact. But regardless of the type of force used, all homogenizers aim to mix together two naturally immiscible substances so that any number of the above benefits can occur.

 

Ready to Learn More?

Homogenizers do a lot of different things, making them useful tools that foster a myriad of advantages across several separate industries. If you need assistance determining the type of homogenizer best suited to your needs, contact our team at BEE International. Our homogenizers combine high pressure with turbulence, cavitation, impact and shear forces, which, when coupled with our patented Emulsifying Cell (EC) technology, serves to improve manufacturing and laboratory processes and saves you valuable time and money.

 

And for additional information, feel free to download our complimentary eBook, “How to Achieve Efficient & Consistent Particle Size Reduction." In it, we detail how our proprietary in-line processing technology maximizes particle size reduction in fewer passes and helps you maintain cost-effective manufacturing practices.

 

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When is the Right Time to Purchase an Industrial Homogenizer?

Posted by David Shechter on Jun 12, 2019 9:00:00 AM

Industrial HomogenizerIn manufacturing terms, bigger is often better, especially if you’re talking about the equipment that companies need to process the products they sell. Indeed, profitability frequently depends on a company’s ability to meet consumer demand while simultaneously maintaining quality and safeguarding costs. Many accomplish this by utilizing large capacity processing equipment to render bigger outputs in less time. By increasing product volume, decreasing manufacturing duration and maintaining the integrity and reliability of both via automated (not manual) processes, companies in all types of industries can generate bigger financial gains.

 

For those industries tasked with creating homogenized products, large scale equipment can facilitate better micronization, better mixing and better scalability (i.e., better overall results). If you’ve ever struggled to meet the demands of your customers or to verify the accuracy, efficacy, constancy and consistency of your products, then investing in an industrial homogenizer will likely solve a lot of your problems. Here’s how you’ll know it’s the right time to purchase an industrial homogenizer:

 

You Have High Product Demand

Industrial homogenizers are created specifically for bulk processing. Whereas laboratory homogenizers are perfectly capable of (and generally preferred for) handling small batch loads, industrial homogenizers can accommodate large quantities of materials, making it easier for companies to process a lot of product in less time. If you’ve moved past the initial research and development stage and have a final product to mass produce, it’s the right time to buy an industrial homogenizer.

 

You Need Flexibility for Processing

An industrial homogenizer is usually more versatile than a laboratory homogenizer, with advanced options available for tailoring processing functions according to individual need. At BEE International, our industrial homogenizers incorporate our patented Emulsifying Cell (EC) technology, a linear process that guarantees scalability of results each and every run. Our machines also include proprietary software, the “most advanced, reliable, and versatile intensifier technology on the market” and an automation system that allows for continuous operation, advanced data collection and integration with plant controls, generating countless opportunities for customization.

  

You Want To Minimize Costs

Bigger machines can cost more and warrant more maintenance. However, our DeBEE 4000 industrial homogenizer model comes equipped with hydraulically-driven vertical intensifier pumps that effectively lower maintenance costs and a “unique CIP position [that enables] flow through the high pressure cylinder for faster and more thorough cleaning.” Both of these features help keep our machines running smoothly and cleanly. Oftentimes, smaller homogenizing systems become overstressed trying to accommodate the processing of an unrealistic load. This not only wears out the machine’s mechanics, it requires more labor, more maintenance and probably more repair, all factors that accumulate more expense in the long run. A BEE International industrial homogenizer can mitigate that problem.

 

You Must Verify Safety Compliance

Manufacturing plants are required to adhere to certain sanitary and safety guidelines. Thus, most industrial homogenizers support compliance with FDA, CGMP and 3-A standards. Our BEE International industrial homogenizers offer this feature, as well as additional ones meant to make cleaning machine parts easier and more effective so that you can adhere to industry standards with minimal disruption to the production process. You should purchase an industrial homogenizer if you are required to verify any trade and/or government guidelines.

 

Want to Learn More?

Homogenizers are essential expenses for companies needing to homogenize and/or reduce the size of the particles in their product(s). Industrial homogenizers, in particular, can help large companies meet manufacturing demand, validate quality and ensure product stability and performance. All our equipment comes with a two year warranty to further protect your investment, your time and the trust of the people you serve. Contact us to learn more.

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Homogenizer Mixer: 3 Benefits for Your Application

Posted by David Shechter on May 2, 2019 4:43:57 PM

homogenizer mixer benefitsMixers come in a variety of shapes and sizes, from the common wire sauce whisks found in most home kitchens to the large drum mixers used in many industrial plants. Choosing one type over the other really comes down to what and how much you’ll be mixing, as well as how often you’ll be doing it. For example, a whisk is an appropriate tool for mixing cake batter, but you’d never try to mix cement for your new patio with one. And if you own a commercial bakery, you’d probably want an electric heavy-duty floor mixer that’s capable of stirring enormous amounts of ingredients day after day instead of a tiny whisk you have to beat yourself.

Another type of mixer is the high pressure homogenizer. At BEE International, our homogenizers pull product through a proprietary system that produces a constant pressure up to 45,000 PSI and pumps out a “high-velocity jet strong enough to cut metal.” The jet then enters our patented “Emulsifying Cell (EC)” and combines with other forces to yield a final product that is thoroughly broken apart and blended. Unlike other types of mixers, a BEE International homogenizer is versatile across many different applications. Here’s why:

Smaller Particle Reduction

Different mixers use different forces to break apart particles. For example, stirrers and agitators use turbulence; sonic mixers use cavitation; high shear mixers use shear; bead mills use impact; and high pressure homogenizer mixers use pressure. High pressure homogenizer mixers effectively reduce product particle size on their own, outputting products with better sensorial and biophysical properties with longer shelf lives. However, BEE International homogenizer mixers combine high pressure with all of the aforementioned forces, facilitating a gentler cell disruption and even smaller particle reduction than any other one mixer type. Operators can adjust the duration, intensity and type of forces used in the mixing process, ensuring that the cell walls of different biological materials are lysed in ways that preserve their intercellular components and

create a "tighter distribution of smaller particles [...] in fewer passes." This not only improves manufacturing efficiency, it reduces manufacturing costs!

Stable Emulsions and Dispersions

In addition, high pressure homogenizer mixers are so adept at reducing particle size that they also more successfully combine materials that don’t normally mix. The unique variety of forces used in BEE International homogenizer mixers especially combine to generate clearer nanoemulsions in all liquid products (emulsions) and more uniform distribution of solids within “liquid solutions, suspensions and colloids” (dispersions). Other mixers that only use one type of force can’t get particles small enough to enable that benefit.

Improved Pasteurization Results

Similarly, while high pressure homogenizer mixers can be used to remove pathogens from various types of food, BEE International homogenizers have the added benefit of allowing hold times under pressure to be customized. This means manufacturers can achieve maximum pasteurization results for a variety of products with only one machine. It also means operators can target specific organisms by applying the least amount and length of pressure necessary to kill each one, thereby preserving other vital components of the final food product for better taste, nutritional value and appearance.

Want to Learn More?

High pressure homogenizer mixers offer manufacturers and researchers a variety of benefits. They produce stable products with longer shelf lives, improved bioavailability and better rates of dissolution. And they do it with less waste and less resources since high pressure homogenizer mixers require less chemical and thermal processing to achieve maximum results. If you want to save time and money while still making superior products, contact our team at BEE International. We are industry leaders in quality homogenizing systems and can help you pick the right equipment for your own distinct needs.

You might also wish to download our free eBook, “How to Achieve Efficient & Consistent Particle Size Reduction,” for more tips on maximizing your manufacturing processes (as well as your ROI) with high pressure homogenizers.

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Particle Size Reduction Equipment: Why Homogenizers Stand Out

Posted by David Shechter on Feb 9, 2019 10:00:00 AM

particle size reduction equipmentReducing large materials into smaller ones is a common and often necessary occurrence in the natural world. From the earth itself eroding to food being digested to rocks being broken into tools, the physical process of reducing something into smaller bits frequently permits advantages (or wonders!) that otherwise wouldn’t exist. Take, for instance, the splendor of the Grand Canyon or the awe-inspiring fact that our bodies can break apart food and air and water into life sustaining nutrients or the many ways humans have made valuable tools from rocks and wood and other resources. Indeed, the world’s very first tool — the hammerstone — was made by ancient hominids and served a purpose not unlike today’s own particle size reduction equipment: to turn something bigger into something smaller.

Modern particle size reduction equipment is categorized according to four basic types:

  • Crushers - which break apart large particles into smaller ones
  • Grinders - which further crumble crushed particles into powder
  • Ultrafine Grinders - which pulverize ground powder into pieces 1-50 micrometers in size
  • Cutters - which yield particles of a specific shape and size

Considering product hardness, consistency and volume (many machines are better suited for certain capacities), companies may use one or more of the equipment types. Many pharmaceutical companies, for instance, opt for ultrafine grinders that can reduce active pharmaceutical ingredients into superfine particles to increase bioavailability. Baby food and soup manufacturers, on the other hand, might need cutting capabilities to ensure that all food pieces in their products are similarly sized. Some manufacturers might use crushing techniques followed by grinding ones or run their products through one system multiple times. It really just depends on the overall purpose of the product being manufactured, the initial ingredient composition and how much money a company has to spend.

With all this in mind, savvy manufacturers started considering the use of high pressure homogenizers as a fifth alternative for businesses in need of particle size reduction equipment. Homogenizers effectively mix together two normally immiscible substances using high pressure. A happy result of the process: homogenizers also reduce particle size! For manufacturers looking to make an equipment investment that not only works, but works across numerous applications, a high pressure homogenizer has become a solid choice. Unlike traditional particle size reduction equipment that only makes use of one type of mechanical force, a high pressure homogenizer utilizes several different ones. And at BEE International, our proprietary system not only uses turbulence, cavitation, high shear and impact to break apart product, it also has modular components that can be customized for specific purposes. Turbulence and cavitation treatments can be altered, shear times can be reduced or lengthened and impact and intensity can be tweaked. So, just want does all this mean? 

The Benefits of Using High Pressure Homogenizers (HPHs) as Particle Size Reducers:

  1. HPHs produce tighter distribution of smaller particles.

  2. HPHs achieve better particle reduction in fewer passes.

  3. HPHs increase manufacturing efficiency.

  4. HPHs reduce manufacturing costs.

  5. HPHs help create products with longer shelf lives, increased bioavailability and improved sensorial and biophysical properties. 

Want to Learn More?

Homogenizers can be used for a lot more than milk production and laboratory tests. Manufacturers in nearly every industry can use them to accurately reduce product particle size with a flexibility unmatched by other equipment types. Contact our team at BEE International for additional information on the ways a high pressure homogenizer can streamline your manufacturing process, improve your product integrity and benefit your bottom line.

Please feel free to also download our free eBook, "How to Achieve Efficient & Consistent Particle Size Reduction,” for more tips.

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2 Types of High Pressure Pasteurization Equipment

Posted by David Shechter on Jan 17, 2019 7:06:25 PM

two types of high pressure pasteurization equipmentIn the mid 19th century, Louis Pasteur helped France safeguard its beer and wine industries by establishing a manufacturing process that preserved and protected alcoholic products from spoilage during export. The principles of that system, eventually known as pasteurization, were based on his observations not only that microorganisms could change the molecular makeup of substances, but that they could be killed when exposed to high temperatures. Coupled with the scientific significance of French chef Nicolas Appert’s previous research on sterilization and canning techniques, thermal pasteurization quickly became the de facto approach for food manufacturers wanting to guarantee the safety and preserve the quality of their liquid and some semi-solid products.

The only problem: exposure to extreme temperatures often changes the way a food tastes, as well as its nutritional value. Believing that neither should have to be sacrificed because of safety and preservation concerns and buoyed by additional scientific advances, as well as increasing public interest in clean eating, more and more manufacturers are starting to use a high pressure processing system (HPP or pascalization) instead of heat to pasteurize their products. HPP effectively removes most pathogens and other harmful microorganisms and extends shelf life, but does not alter a food’s nutritional content, its color or its flavor. Take a look at the two most common types of high pressure pasteurization equipment: 

Water Tanks for Prepackaged Food Pasteurization

Certain prepackaged liquid and solid food items can be pasteurized using a large steel tank filled with a liquid (usually water). After the packaged food is loaded in to the tank, pumps apply pressure that changes the food’s cell structure, effectively inhibiting cell division and killing most microorganisms. According to food science research at Rutgers University, “vegetable and fruit products such as juices, salsa, dressing and guacamole; meat products such as ready-to-eat deli meats and poultry; and seafood such as shellfish and fish products” can all be processed using a high pressure pasteurization technique. The tank method is capable of processing a lot of food at one time, making it a good choice for manufacturers with high volume productions.

Homogenizers for Liquids and Semi-Solid Pasteurization

Liquids and some semi-solid products can also be pasteurized with high pressure homogenizers that push them through a series of tubes using a hydraulic pumping system that creates pressure and sterilizes them. The homogenizer then expels the product through a small opening with a drop in pressure that forces cell rupture, breaking the product apart into equally sized pieces that can be easily and uniformly mixed. Combining the pasteurization and homogenization process allows manufacturers to more efficiently manage their time, reducing their energy use and waste, and serves as a viable alternative to thermal pasteurization in the milk and juice industries especially. 

Want to Learn More?

High pressure pasteurization equipment improves the shelf life of products and maintains food flavor and nutritional value. At BEE International, our high pressure homogenizers also provide the added benefit of completely dispersing the particles of your products, allowing for uniform texture in addition to taste. This means you can use our homogenizers to enhance more than just food products. Our high pressure pasteurization equipment improves pharmaceutical, chemical, biofuel and cosmetic manufacturing and aids in RNA extraction and oil emulsification processes. Contact us to learn how it can help you, too.

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Homogenizers for Cannabis: How To Deliver More Consistent & Uniform Particle Size

Posted by David Shechter on Nov 29, 2018 11:30:00 AM

homogenizers for cannabisIf you’re looking into cannabis processing options, homogenization might seem a bit complicated at first. While there are certain elements to homogenization that are technical, homogenization is actually a pretty simple process! When it comes to particle size reduction, homogenizers work to combine two or more ingredients in a uniform manner. Since homogenization is mechanical, it doesn’t involve adding preservatives or other materials to the mixture. Thanks to pressure and other forces, particles become uniformly dispersed in the homogenization process.

When it comes to cannabis products, one of the best benefits a homogenizer can offer is how they deliver more consistent and uniform particle sizes in their dispersions. Here are the top three reasons why this should matter to you:

1. No More “Hot” and “Cold” Spots

As mentioned above, when you homogenize two or more ingredients, those particles are reduced to a small size and uniformly dispersed throughout the mixture. So without homogenization, there’s not a good way to determine where the cannabinoids are in your products. Therefore, you may have one item that is chock full of cannabinoids while the next item doesn’t have any. In fact, a recent study found 31% of cannabis products tested did not contain the amount of cannabinoids their labels claimed. That’s an unacceptable number in any industry, but can be particularly embarrassing for companies that are looking to establish themselves in the relatively new commercial cannabis industry.

2. Increased Drug Efficacy

Next, a smaller particle size will have a greater total surface area which can increase the rates of dissolution and absorption of any active ingredient. Therefore when working with cannabis, you need to ensure your products have small, uniform particle size for a greater absorption rate and there’s no better way to do this than using a homogenizer. Homogenizers can help you reach your target particle size reliably plus you can run a filter through the end product to see that all particles are the target size. Further, smaller particles can increase bioavailability by up to 75% by increasing the surface area and you can actually reduce the active ingredient required to achieve the same effect.

3. Product Appearance

While this doesn’t apply to cannabis products alone, a smaller, more uniform particle size can be beneficial for aesthetic reasons. When particles are homogenized properly, the end product can appear smoother and more homogenous. Therefore, when looking to add cannabis to lotions, food products, and other items, having a beautiful product can make all the difference when it comes to sales.

Final Thoughts

There’s a long history of businesses across all industries using homogenizers to guarantee uniformity in their product, which is something that everyone in the cannabis industry should want when creating new products. Plus, consumers want to associate with brands and products they can trust. So when you use high-pressure homogenizers in your process, you'll be keeping the promises you already make to your customers.

To learn more about CannaBEE, our homogenizer solutions customized for cannabis, check out this page for more information or download our free eBook:

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How to Improve RNA Isolation with Homogenization

Posted by David Shechter on Nov 15, 2018 11:30:00 AM

rna-isolationRNA isolation is, unsurprisingly, a delicate and complicated process that requires diligent execution and precise application. Indeed, even seemingly small mistakes can completely ruin an attempt to extract, isolate, and study RNA samples. With that in mind, today we’re here to explain how homogenization will help improve RNA isolation –– while also sharing a few best practices all professionals can use to boost their results when working with RNA. Check them out below:

Sample Collection and Preservation

Researchers value RNA analysis in no small part because studying RNA can help explain how genes function and are expressed. Regardless of the aim of a given experiment or project though, collecting and preserving samples in the right way is essential to generating viable results with RNA. Some tissues are notoriously difficult to extract RNA from (like bones or roots) and if samples aren’t preserved properly before homogenization, the results of a study can also be affected. As such, researchers should strive to either 1) conduct homogenization immediately following sample collection, which may not always be possible, or 2) freeze the samples using a substance like liquid nitrogen for temporary containment until homogenization can be performed. Note here that thawing frozen samples before homogenization will also affect the outcome of an experiment.

Benefits of Homogenization

The good news about utilizing homogenizers in regard to RNA extraction is that they eliminate the potential of heat production –– since high-pressure homogenizers operate so quickly. In addition, homogenizers are scalable and compatible with a wide array of sample sizes and types. Even if a samples has cell walls that are hard, or when other conditions aren’t ideal, homogenizers can still assist researchers in achieving meaningful yields. Lastly, homogenizers are key to separating samples from RNase, which as we’ll explain below, will significantly alter RNA samples and often render them unusable.

Preventing RNase Exposure

Ribonuclease or RNase, presents a problem for researchers looking to study intact RNA. RNase is an enzyme that catalyzes the degradation of RNA into smaller pieces. This is obviously something researchers want to avoid. Unfortunately, RNase is more or less everywhere, which means that it’s easy to expose a sample to it through lab equipment or on the surface of gloves. Professionals can use special cleaning solutions to ensure their equipment doesn’t spoil their experiment thanks to RNase exposure–– and should change their gloves often throughout their work. Remember also that proper storage at the end of the isolation procedure is also imperative to prevent any outside factors influencing results. RNA is generally stored at low temperatures –– either -20 Cº or -80 Cº depending on how long the storage will last. 

Final Thoughts

With the right equipment and by following guidelines and best practices to the letter, researchers can glean meaningful RNA yields under adverse conditions. At BEE International, we’re passionate about providing scalable, highly effective homogenization solutions for our business partners. We strive to offer the finest products –– and customer service –– in the industry. Don’t just take our word for it, though. Contact us today!

Plus, to get a firsthand look at how our homogenizers enable efficient and consistent particle-size reduction, check out our free eBook here:

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Why We Love High Pressure Homogenizers (And You Should, Too!)

Posted by David Shechter on Aug 30, 2018 11:30:00 AM

Love High Pressure HomogenizersIf you were asked about some of your favorite products, a high-pressure homogenizer may not make the list. However, as we’ve discussed in a previous blog, the homogenization process produces a lot of products you most likely use and consume in your daily life. That’s one of the reasons we here at BEE International love homogenizers…but there are more! Let’s take a look at some of the most popular uses of high-pressure homogenization and the benefits they offer consumers.

Particle Size Reduction

High-pressure homogenization utilizes mechanical force to break down particles or droplets in a liquid into smaller and more uniform sizes, resulting in a dispersion, which is where fine particles of one substance are scattered throughout another substance. Dispersions can be further classified into solutions, suspensions and colloids, a mixture somewhere between a solution and a suspension.

Some high-pressure homogenizers can reduce particle size to as small as a single-digit nanometer. Plus, they ensure the composition is equal throughout, and the overall molecular makeup of the sample isn’t compromised. Many high-pressure homogenizers are able to produce emulsions, dispersions and suspensions at particle sizes of 100 µm or higher, and a select few can achieve a size of down to 0.1 µm. Key benefits of particle size reduction include:

  • Increased dissolution rate.

  • Improved drug delivery.

  • Controlled rate of reaction.

  • Increased density.

  • Consistent texture and product appearance.

  • Enhanced sedimentation stability.

  • Improved mineral processing.


Important Applications

Food and Beverages

Though one of the most well-known uses of high-pressure homogenization in the food and beverage industry is for milk, it is also widely used in the manufacture of sauces, spreads, salad dressings, fruit juices and concentrates, fish and meat pâté, sweeteners, baby food, egg product, other dairy products and more. It helps companies utilize to efficiently, cost-effectively and safely produce their goods. Food homogenizers also aid in the delivery of a product with uniform texture, flavoring and taste through a scalable process that allows for a longer period of freshness than food comprised of larger particles.

Pharmaceuticals, Chemicals and Biofuels

High-pressure homogenizers are used for numerous products from the chemical and biotechnology industries and products such as pharmaceutical tablet coatings, vaccinations and creams. They can be used to create cost effective medicines, modify microorganisms, synthesize new chemicals and develop alternative biofuels.

Cosmetic Creams and Lotions

Due to its ability to provide potent particle size reduction, enriched texture and consistency and strengthened emulsion stability, high-pressure homogenization is a preferred method for improving the production of cosmetic creams, lotions and other cosmetic products. It is especially beneficial for beauty creams and moisturizing lotions because they have to have a combination of the right texture and consistency in order for the skin to be able to effectively absorb them.

RNA Extraction

 Utilizing homogenization for RNA extraction enables processing to be effectively and repetitively processed in only seconds, thereby eliminating heat production. Its force and high pressure produce consistent and uniform samples, and it can process both small and large samples. Plus, homogenizers are scalable and compatible with many sample types, including those with tough cell walls. Using a powerful chaotropic salt solution, RNA extraction purifies RNA from biological samples by quickly disrupting cells or tissue to release the nucleic acids from the cells. These released RNAs are valuable in providing details on which genes are expressed (used to make products), to what degree the genes are active, how they work to regulate each other via small, regulatory RNAs and which structural RNAs (i.e. rRNAs, mRNAs and tRNAs) are produced.

Oil Emulsification

 Due to the fact that water and oil are immiscible, powerful mixing is essential to conducting oil emulsification. Therefore, specialized machines such as high-pressure homogenizers are preferred because they not only impart high shear but also are able to reduce particle sizes much more efficiently than other blending and emulsification methods. They are used in a wide variety of research and scientific uses and by many industries that require the use of active ingredients because they reduce active ingredients to a small enough size to ensure a thorough dispersion throughout the medium produced. Additional benefits of high-pressure homogenization for oil emulsification include production of stable emulsions, improved product consistency, shelf-life, texture, color and flavor and the ability to overcome the resistance caused by two immiscible liquids.

BEE International: Our Love for Our Products Shows in the Way We Treat Our Customers

BEE International offers homogenizers that are both high-quality and reliable. Our line of equipment is suitable for virtually every industry and can work in just about any environment. Contact us today to learn how our products can maximize your homogenization processes.

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The Ultimate Cheat Sheet for Choosing a Cell Homogenizer

Posted by David Shechter on Aug 16, 2018 11:30:00 AM

Businessman is standing in front of two direction signs.If you attended high school before laptops and tablet computers replaced books and used a landline to make phone calls, you’ve probably heard of CliffsNotes. Often used to help students figure out the intricacies of a novel that was required reading for English class, these handy pamphlets were a sort of a study guide highlighting the work’s key points.

In this blog, we’re offering you a CliffNotes version of tips for selecting a cell homogenizer that best meets your business needs. We’ll outline cell homogenization, often referred to as cell lysis, offer key factors for how best to achieve it and provide some benefits on it.

What is Cell Lysis?

Cell lysis, which takes place in biotech, pharmaceutical, food, cosmetic and chemical laboratories on a regular basis, is the rupture of the cell membrane resulting in the release of cell contents and the subsequent death of the cell. Research scientists and laboratory technicians disrupt the cell’s structure to extract organelles, proteins, DNA or mRNA. 

Cell lysis can be achieved through mechanical or chemical methods. Mechanical cell lysis techniques include high shear mixing, traditional homogenization, sonication, grinding, freeze/thaw cycles and high-pressure homogenization. Chemical cell lysis techniques include osmotic lysis and the use of detergents, chelating agents or chaotropic agents.

In order for cell lysis to be performed successfully, the following factors should be present:

  • Intracellular proteins should be accessible for extraction and solubilization.
  • The process should be flexible, as different cells require different cell lysis strategies.
  • The technique should result in a high yield in a short time.
  • The process should be relatively easy to perform.
  • Results should be consistently reproducible and scalable.

 

How Homogenization Helps

Of the methods used to achieve cell lysis, the most widely used is homogenization, which offers a multitude of benefits for production laboratories and the consumers who buy their products. It is especially useful for small volumes of cells and/or cultured ones and involves the use of shearing force on the cell. By forcing a small cell through an even smaller-sized orifice, homogenization removes the outer layer and lyses the cell. Some cell lysis products use utilize shear, and others employ sonic cavitation. However, the best choice when purchasing a homogenizer for cell lysis is one that uses shear, cavitation and impact and lets the user adjust the relative amount of each force for different applications.

The homogenizer you choose for cell lysis should be scalable to allow for customization in pressure and intensity, resulting in both cost and time efficiency for the user. It also must accommodate for multiple cell types and adjust for various sample sizes. Other factors to consider when selecting the right cell homogenizer are the force required to produce the highest yield in the least amount of time and the volume, efficiency, stability and sanitary standards compliance of the product. Advantages of using this type of homogenizer are:

  • Accessibility of intracellular proteins.
  • Built-in process flexibility.
  • Ease-of-use and low learning curve.
  • Reproducible and scalable results.
  • Various sample size processing.
  • Ability to disrupt all cell types.
  • Better results in fewer passes.

BEEI International: Bringing the Best Homogenizers to You

BEE International offers numerous high-quality homogenizers to meet the needs of any industry for cell lysis. Our laboratory-grade models include the Nano DeBEEMicro DeBEE and Mini DeBEE, all of which are easy to use, provide consistent and reliable results and easily scale up for full scale production. Plus, they offer maximum experimentation capabilities and the ability to change flow, pressure, shear and impact settings. Contact us today to learn more about our full line of homogenizers.

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Making the Right Choice: Homogenizer Manufacturers

Posted by David Shechter on Jul 31, 2018 11:30:00 AM

choosing high pressure homogenizerShopping for certain products is easy. If you want a fast food hamburger, you have a few well-known establishments from which to choose. If you’re looking to purchase some products to stain a deck or tend to weeds in your lawn, you most likely go to your local hardware store or one of a couple bigger, nationwide home improvement stores.

Investing in a more expensive product often requires more research and comparison. Usually, you can read customer reviews online or find ratings published by consumer organizations. When purchasing a homogenizer, however, it may be harder for you to obtain the advice needed to make the right decision.

First, let’s do a little refresher on homogenization. The primary methods used for homogenization include ultrasonic, mechanical and high-pressure, and homogenizers are used across multiple industries, such as:

  • Pharmaceutical: vaccines, antibiotics, cancer treatment and medicinal creams.
  • Food and Beverage: mayonnaise, ice cream, cream liqueurs, salad dressings, dairy products and more.
  • Biotech: creation of cost-effective medicines, modification of microorganisms, synthesization of chemicals and development of alternative biofuels.

Based on these details, the first step in finding a homogenizer manufacturer in asking yourself the following questions:

  1. Why do I need one?
  2. What differentiates one homogenizer from similar ones on the market?
  3. Does my product require a specific particle size?
  4. Which accessories are recommended and/or required for optimal performance?
  5. What motor power does my product require?
  6. Do I have a desired tube capacity and/or mixing speed?

By understanding the available options and what can best help you accomplish your specific homogenization goals, you are better equipped to select a manufacturer to meet your specific needs. It’s important to choose a manufacturer who will work with you to customize a homogenizer that delivers the best possible results. Some companies are able to customize the forces in their homogenizers to suit your application. Some even produce separate homogenizers for each phase of drug development.

Consistency is essential in homogenization, so employing the services of a manufacturer who aids you in reproducing reliable results is imperative. They should offer a homogenizer that is easy to scale and enables the product developer to quickly apply a wide variety of mixing techniques to a formulation. The manufacturer should have a reputation for reliability, offer customer referrals and provide you with at least once demonstration of their product(s).

Once you’ve decided on a manufacturer from which to purchase your homogenizer, factors to consider when choosing a brand and model include:

  • Sample types.
  • Maximum operating pressure. 
  • Mixing speed.
  • Processing setup. 
  • Processing range. 

 

BEE International: The Right Choice for the Best Reproducible Results

Our technology achieves superior results for nano emulsions, cell lysis, uniform particle reduction and related applications, and we offer homogenizers that are high-quality, customizable and reliable. Plus, we provide unparalleled customer service from demo to purchase and beyond. Contact us today for more information on our entire line of homogenization equipment.

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