The homogenization of cells refers to the process by which all components of a biological sample are made equal. By breaking down cell membranes/walls, it allows for the thorough mixing of a sample’s cellular contents. The result: a sample that has a consistent and uniform molecular make-up. Here’s everything you need to know about cell homogenization:
The Homogenization Process
The homogenization of cells is basically a cell lysis technique meant to release the intercellular contents of cells so that they can be better accessed, maintained and/or mixed. There are other ways to disrupt (or lyse) cells, such as cryopulverization, nitrogen decompression and more, but the most popular method is homogenization. Homogenization uses one or more types of mechanical force to break apart and mix the components of a sample. When used with biological materials, cell homogenization allows scientists to isolate and study the contents of cells and manufacturers to make better products.
Why You Should Homogenize Cells
There are many reasons to choose cell homogenization over other cell lysis methods, the most significant being that different types of cells require different disruption techniques. For instance, some cells are fragile and need a more sensitive means for cell lysis lest the entire cell be destroyed during the process. Some cells have tough outer matrices or walls, warranting a more extreme cell lysis force in order for valuable intercellular contents to be reached. Some cells need enzymes or other reagents added to them to facilitate lysis, and in other cases, the cell lysis procedure itself either creates or relies on a certain temperature that can comprise or otherwise negate optimal results. The considerations are varied and wide-ranging with everything from the cost of the equipment, the size of the sample and stability, reproducibility and scaling problems to even physical space constraints (to accommodate the size of the equipment) also affecting the selection of one cell lysis method over another.
The Value of High Pressure Homogenization of Cells
Here at BEE International, we believe that homogenization — specifically, high pressure homogenization — affords the best value for many scientists and manufacturers alike. Our high pressure homogenizers offer a customizable approach to the cell homogenization process, with our patented Emulsifying Cell (EC) technology allowing you to control the type and duration of the force(s) used according to multiple cell types. Indeed, our proprietary homogenizing system can rupture cell walls and membranes without damaging any other crucial cell components, making our equipment effective, efficient and extremely competitive. If you would like to learn more about the homogenization of cells and how high pressure homogenizers can improve your productivity, as well as strengthen the quality of the products you test and create, please contact us. We believe cell homogenization offers countless benefits when used as a part of many research and manufacturing processes.