Why Cell Disruption is More Efficient Using Homogenization

Posted by David Shechter on May 27, 2022 9:30:00 AM

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Cell lysis (also called cell disruption) is a critical part of many laboratory and manufacturing processes. It is the method by which a cell wall or cell membrane is broken so that the cell’s intracellular contents are released. The objective of any cell disruption procedure is to get as much viable material (molecules and other particles of interest) from the cellular fluid so that it can be studied and/or used for other purposes. 

There are several tools that help facilitate cell disruption, including ultrasonication, mechanical grinding, freeze-thawing, osmotic shock and more; however, homogenization is the only cell disruption method that is efficient for the widest range of applications.

What is BEE International’s Homogenization Technology?

BEE International are experts in high pressure homogenization, which is used to break particles apart and to facilitate cell lysis. Specifically, it involves the use of fluid pressure to force a sample — in this case, any type of cell — through a narrow passage of different-sized orifices. As the cell traverses the system, it is acted upon by a variety of additional forces depending on the velocity of the fluid’s flow (which can be manipulated for any type of cellular material, whether it’s plant or animal, algae, bacteria, fungi, etc.). Thus, upon exit from the homogenizing system, each cell has experienced a customizable combination of forces (pressure, turbulence, shear, cavitation, impact and intensity) that has effectively broken it apart and released its intracellular contents in good form.

What Makes BEE Homogenization Technology So Efficient for Cell Disruption?

BEE technology works so well for cell disruption because:

It utilizes a customizable combination of forces that reduces the need for other equipment and steps.

BEE technology supplies constant pressure so that every cell that passes through the pumping system experiences the same combination of shear, cavitation and/or impact forces, which results in reliable, consistent results each and every time. Hard plant cell membranes that are difficult to rupture can be exposed to greater pressure and more force, while animal cells that are often fragile can be treated more gently. This allows manufacturers and scientists to use the same piece of equipment with a greater variety of cell samples.

It is an in-line process

In-line processing is not only more consistent but also saves time, thereby reducing manufacturing costs.

It delivers results that are easily repeatable and scalable regardless of volume.

Homogenization technology delivers reliable scale up from the lab to production due to several key features. In addition to having a customizable PLC panel that controls the level of pressure, temperature and other forces used during any one run, BEE homogenizers utilize extremely powerful motors that convert hydraulic power through high pressure cylinders and make it possible to process reliable results no matter the original sample size or volume.

It preserves valuable intracellular contents.

Oftentimes, chemicals or enzymes in the form of detergents and chaotropic agents are used for cell disruption (particularly when extracting proteins), but these chemicals can denature sensitive proteins and/or require removal before the proteins can be used. Homogenization, however, requires no chemical additives; it also generates little heat, which can similarly alter the integrity of intracellular contents. By eliminating the need for chemical detergents and reducing the potential of other harmful by-products (like heat and too much rigor), microfluidization ensures that cell lysis always results in a usable biological sample that most represents its natural form.

The BEE International Advantage

While BEE high pressure homogenizers provide a technology edge for cell lysis, we understand successful manufacturing requires ease of use, reliability and excellent customer service. In short, our homogenizers save our clients money and a lot of time! To learn more about how high pressure/microfluidizer technology works and the specific benefits it can offer you, please contact us.

3 Materials Where Particle Size is Essential for Effective Production

Posted by David Shechter on Mar 25, 2022 11:15:00 AM

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The ability to manufacture quality products depends on the quality of the materials. And quite often the best materials are the ones with a consistent size and distribution of their particles. That’s because the stable distribution of particles directly impacts the physical and chemical composition of the materials they compose, affecting the ability of those materials to function as needed when coming off a production line. Take a look at three materials where particle size is especially essential for effective production:

Food

Today, the majority of the food we eat is manufactured in some way. It has been altered from its natural state to satisfy safety and storage standards and to provide consumers with reliable products that are good to eat every time we purchase them. In many — probably most — of those instances, manufacturers have had to combine several ingredients in order to make these products. Items such as milk, salad dressings, juices, coffee, flour, chocolate, spices and more rely on equipment that reduces at least one of their ingredients to a very small size because breaking particles into smaller-sized pieces increases the surface area of each particle and provides more opportunity for chemical bonding to take place. More bonds mean better cohesion amongst all the ingredients and an extended shelf life for the final food product. Indeed, with all ingredients of a food item thoroughly mixed, it simply tastes better and lasts longer, and its flavor remains consistent with every bite! Small particles frequently improve the nutritional value of the food, as well, especially when nutrients need to pass through bodily membranes and/or preservatives need to be added to food to keep it safe for consumption. Small particles mean the preservatives are not visibly noticed or tasted and the food is still nutritious and safe to eat.

Take, as an example, milk. When smaller in size, the fat globules in milk stay more evenly dispersed; they don’t float to the top of the milk and settle into a layer of cream. They are also easier to digest when they are smaller, meaning the body is able to more quickly access the milk’s nutrients and put them to use to keep us healthy.

Pharmaceutical Ingredients

Particle size is similarly important in the manufacturing of drugs and pharmaceutical products including but not limited to injectables, tablets, sublingual dissolvables, dermal patches, and creams. By reducing the particle size of the chemical components or “API” within pharmaceutical formulations, it allows them to be easily absorbed by the body, enhancing bioavailability. As pharmaceutical products must meet reproducible standards for drug delivery in both efficacy and dosage accuracy, the importance of consistent particle size during production cannot be understated.

Cannabis Products

Traditionally, cannabis and hemp products go through an extraction process where chemical compound rich oil is isolated from plant matter. This oil is considered hydrophobic, which means it does not easily mix with other water-based products. However, by reducing the particle size of the oil droplets and creating a nanoemulsion, it becomes possible to suspend these submicron oil particles in water-based products for prolonged periods of time.

Similarly to pharmaceutical applications, cannabis and hemp products also become more bioavailable through particle size reduction. The human body cannot readily absorb cannabinoid molecules because they are too large to pass through mucus membranes and enter the blood stream. The body first must digest the cannabinoids and THEN absorb them which is why traditional edibles take so long to take effect.

Cannabinoids that have undergone particle size reduction have quicker onset time, are more consistent in active ingredient dosing, have longer shelf life, and are quickly becoming the gold standard method of consumption among users.

The BEE International Advantage

Particle size and distribution are important aspects to consider during the manufacturing of thousands of products. Food, pharmaceutical ingredients, and cannabis products are just a few groups where particle size becomes critical for ensuring stability, consistency, and effectiveness. One of the most efficient and scalable ways to guarantee a tight particle size distribution across the widest array of industries is through high pressure homogenization.

BEE International manufactures top of the line high pressure homogenizers that provide unparalleled process control and allow the user to manipulate all three forces involved in high pressure homogenization (Shear, impact, and cavitation) for extensive formulation discovery and efficiency optimization.

To learn more about our proprietary Emulsifying Cell (EC) technology, please contact us today.

How Better Particle Packing Improves Pharmaceutical Suspensions

Posted by David Shechter on Feb 28, 2022 10:00:00 AM

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Pharmaceutical suspensions are dispersions of insoluble solid particles in a suspending (often liquid) medium. They represent a common form of drug delivery, with pharmaceutical suspensions safeguarding the accurate dosage of many insoluble drug ingredients. Of course, pharmaceutical suspensions taste and/or smell better and are more effective when the size of the solid particles are as small and as uniform as possible; small particles that are similar in size can be more evenly packed, making them more stable and a lot more reliable as chemical agents. Take a look at why controlled particle packing improves pharmaceutical suspensions and the type of equipment needed to render the best particle packing results:

Small, Uniform Particles Improve Pharmaceutical Suspensions

Think about mixing together sand and water, and then about mixing together pebbles and water. Which one is easier to do? It’s sand and water, right? The small size of each sand grain makes it easier to mix them all into the water. It also diminishes their weight, allowing them to stay “floating” in the water for a longer amount of time. In much the same way, pharmaceutical suspensions that have smaller particles in them remain stable (i.e., mixed and “useable”) for longer periods since they necessarily delay sedimentation (which would render the drug ineffective and, consequently, useless). Furthermore, smaller particles help to control the delivery of the drug’s active ingredient by evenly dispersing it throughout the suspending medium, ensuring that each dose contains equal parts of all ingredients. Finally, smaller particles work to keep pharmaceutical suspension costs down since the volume of active ingredient(s) of any one product remains constant and can be consistently measured and dispersed during each manufacturing run.

High Pressure Homogenizers Render the Best Particle Packing Results

There are many types of equipment specifically designed to break apart substances. The industry often determines the type used. When making pharmaceutical suspensions, for example, drug manufacturers frequently choose bead mills, rotor-stator homogenizers and/or ultrasonic homogenizers. The problem, however, is that all of these types of homogenizers only use one force each (such as shear, impact or cavitation). Indeed, each piece of equipment has limitations regarding the type of material it can process and the minimum size of particle it can guarantee. Our high pressure homogenizers offered here at BEE International, however, use a combination of agitation, shear, impact , cavitation and pressure to ensure the most versatile range of applications available on the market today.

The BEE International Advantage

The patented and propietary technology of BEEI high pressure homogenizers combines multiple forces to ensure the most controlled particle size reduction processes. Our equipment not only allows pharmaceutical suspension manufacturers to accurately and consistently render the smallest and most uniform particles, but we also deliver the least variance in particle size distribution in the fewest passes. Typically, users realize at least 10% improvement over other conventional HPH in this capacity.  BEEI empowers users to create the most efficient, thus, more affordable, process for their products.

Please contact us to learn how high pressure homogenizers from BEE International improve particle packing so that the pharmaceutical suspensions made with them are longer lasting, safer and more effective than those made with other types of equipment.

What Is Cavitation and How Does it Compare to Shear Mixing Forces?

Posted by David Shechter on Jan 27, 2022 9:45:00 AM

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Have you ever wondered: “What is cavitation?” If you’ve had a chemistry or biology class you may have encountered the term and learned a little about its meaning in various situations. 

Cavitation is basically the formation and collapse of “bubbles” (i.e., cavities) within a liquid substance that occur due to pressure changes. It can be a harmful process (like when vibrations work to erode mechanical parts), but in many instances, cavitation is a beneficial and often necessary part of certain types of industrial, medical and research processes. Thus, answering the question, “what is cavitation,” helps shed light on the role physics plays in our everyday lives. Here’s what you need to know:

The Simple Answer to “What is Cavitation?”

Cavitation is a type of force. 

As a type of force, cavitation changes energy, the very essence of life. It occurs when a liquid’s vapor pressure rises above its static pressure, causing pockets of vapor to form within the liquid. As the pressure again rises within the liquid, these “vapor bubbles” implode sending out shock waves that are able to break the bonds of surrounding particles. The resulting turbulence from these collisions and the destruction they cause then creates subsequent impact forces, which, in turn, cause even more bonds to disrupt. The result, a substance that has been broken up into tiny (often nano-sized) pieces.

There are Many Types of Forces That Can Be Used in a Variety of Ways

There are many types of forces. All forces occur naturally, but oftentimes humans use one or more types of force to create something or break it down. Doctors, scientists and manufacturers, for example, intentionally induce cavitation via the use of homogenizers in order to cleanse, study, test and produce a variety of substances. Sometimes they use cavitation machines to facilitate multiple types of medical treatments, as well. Together these products and processes include — but aren’t limited to — medications, paint, plastic materials, water purification devices and many different cosmetic, weight-loss and other medical procedures!

How Does Cavitation Compare to Shear Mixing Forces?

Cavitation is often a preferred source of energy force when making or using certain types of products because it’s extremely powerful and able to impact a large number of particles at one time, meaning more products can be made and more cells can be affected (when used with/for biological samples). Other types of forces, like shear mixing forces, can’t render the same effects. 

Shear mixing forces are forces that work in different directions to break apart and mix the particles of a substance. They’re a bit like a knife that chops away at an onion, dicing it at different angles. Because shear mixing forces move haphazardly, they aren’t as powerful as cavitation’s cyclic stress. Produced as the bubbles of cavitation rise, burst and shrink over and over again, cyclic stress is a controlled process, leaving a type of uniform destruction in its wake. Shear mixing forces, on the other hand, which utilize no established or reliable exertion of force, can’t be depended upon to break apart particles with any guaranteed conformity or regularity when employed singularly.

The BEE International Advantage

Cavitation is a useful and necessary process in many industries. It effectively disrupts cellular bonds and creates the smallest-sized particles, ensuring manufacturers and healthcare professionals alike have access to substances that can be better — i.e., more easily — mixed and, thus, making it possible for them to develop products that actually work

Here at BEE International, however, we don’t just recognize the power of cavitation, we aim to maximize it! We’ve designed a patented emulsifying (EC) technology that combines cavitation forces with shear and impact forces to ensure that industries have access to the smallest-sized particles possible so that even better products can be made. Our proprietary blend of multiple forces offers results that surpass the limited effects of shear, impact and even cavitation alone. Furthermore, our equipment is completely customizable, allowing users to intensify processes according to the task at hand. The result: the best high pressure homogenizers on the market today. Please contact us to learn more.

3 Industries Where Ultrasonic Emulsification is Changing the Game

Posted by David Shechter on Dec 28, 2021 10:45:00 AM

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Many industries depend on ultrasonic emulsification to get their products out the door and in front of customers. Put plainly, ultrasonic emulsification is basically vigorous mixing, and many industries use it because it is the only way to facilitate the combining of certain types of substances: those that are naturally immiscible, like oil and water, for example. Keep reading for a quick overview of ultrasonic emulsification, the industries that rely on it and info on an alternative piece of equipment that can vastly improve the process:

What is Ultrasonic Emulsification?

As mentioned, ultrasonic emulsification is a powerful way of mixing two liquids so that one is completely dispersed throughout the other and neither will revert back to its original properties over time. Of course, there are other ways of facilitating emulsification, but ultrasonic emulsification relies on ultrasound energy (high frequency vibrations that cause cavitation) to forcibly combine the two substances. Think about many popular food items, like salad dressing; you know that oftentimes you need to shake the bottle before pouring in order to evenly blend the dressing’s ingredients and not end up with a bowl full of oil (or vinegar) only! Ultrasonic emulsification is just a high-tech method of performing the same process. Since oil and water do not mix, (no matter how much shaking or cavitation is applied) an emulsifier or surfactant, an additional ingredient that mixes well with both water and oil, is added so that two substances mixed with the right mixing technology can stay mixed in a homogeneous state.

Industries that Depend on Ultrasonic Emulsification

In addition to the food industry, there are many other industries that create common and highly useful products via ultrasonic emulsification.

  • The Pharmaceutical Industry

In the pharmaceutical industry, it is often necessary to create oil-in-water emulsions in order to make medicines more palatable and to increase their efficacy by evenly dispersing their active ingredients. Without ultrasonic emulsification, for instance, many drug products would remain in an unmixed state, too bitter to ingest and/or unable to work effectively since they would inevitably deliver inconsistent dosages of the ingredients needed every time they were taken. It's just like that bottle of dressing that was not shaken before being poured and therefore spurted out alternating globs of oil and vinegar but never an actual vinaigrette.

  • The Beauty Industry

Similarly, most cosmetics, perfumes, and skin and hair care products need to undergo some type of emulsification process to ensure that they look appealing and can be applied evenly across the skin. Ultrasonic emulsification, in particular, simultaneously mixes and breaks apart substances into incredibly small particles (more so than mere mixers or blenders), enabling manufacturers to better guarantee that their products will possess a smoother consistency and be more easily absorbed into the skin. Furthermore, substances with smaller particle sizes are more stable, meaning they last longer than products that haven’t been properly mixed or have been mixed using only mechanical means.

  • The Cannabis Industry

Perhaps, however, the cannabis industry is currently reaping the best results as a result of their emulsification processes. In this rapidly growing market, the best-tasting and most potent cannabis products are the ones in demand. Ultrasonic emulsification allows manufacturers to guarantee a consistent product each and every time it leaves a production line since it reduces the size of cannabinoid molecules, making it easier for them to be absorbed by the body and also delivering a more uniform amount of active ingredient with every swig, bite, puff, swallow, chew, rub, spray or any other type of ingestion/application!

The BEE International Advantage

There’s no doubt that emulsification has changed the game for countless industries, allowing them to create products that last longer, taste better and/or provide better and more consistent effects than those that haven’t undergone the same type of robust processing. 

There is an even better alternative, though: high pressure homogenizers (HPH). Our HPHs here at BEE International, for example, utilize a patented Emulsifying Cell (EC) technology that surpasses the limited cavitation capacity of ultrasonic equipment and also employs other mixing forces of turbulence such as shear and impact. Control levels of each of these forces is in the hands of the user with BEE HPHs which means you can control the most efficient process for your product and the very best efficiency yield. Products emulsified with our homogenizers possess the tightest distribution of the smallest possible particle sizes and provide even higher quality products than ultrasonic mixers, sonicators, or other types of sonic mixers. Unlike sonication, BEE's proprietary homogenizing processes can scale up with ease as all our technology is built on the same platform (from R&D or Lab to pilot to manufacturing). Ultimately, this means companies spend less time and less money during their process manufacturing!

There’s a reason we’re called BEE; we offer the “Best Emulsifying Equipment” on the market. To learn more about our products and the technologies that make our HPHs the best option for your own emulsifying needs, please contact us today.

What’s the Best Way to Lyse Bacterial Cells?

Posted by David Shechter on May 28, 2021 9:10:46 AM

the-benefits-of-a-laboratory-homogenizer-for-cell-lysisCell lysis is the process by which a cell’s membrane is ruptured so that the cell’s contents are released. It can occur naturally (such as when certain viruses cause infections that lead to cell rupture and death) or it can be induced artificially. In addition to scientists who wish to study and experiment with intracellular contents, and thus, must be able to control the cell lysis process, there are multiple industries (including, but limited to, the pharmaceutical, biotech, food and chemical industries) that need to lyse cells in order to make products. To ensure their products’ safety, quality and efficacy, these industries must intentionally disrupt cell membranes according to their own standards instead of waiting on any natural lysis sequences that mayor may not occur. Indeed, one of the most critical components of any manufacturing process is ensuring safety, removing all potentially harmful substances so that the wellness of consumers is always protected. To this end, many industries use artificial cell lysis to remove pathogens, of which some of the most frequently occurring are bacterialcells. The question then becomes: what’s the best way to lyse bacterial cells, essentially killing them and rendering them incapable of causing illness and disease? Here’s the short answer:

Bacterial Cell Lysis is Best Facilitated by BEE International High-Pressure Homogenizers 

While there are several ways to artificially lyse cells (sonication, chemical lysis and traditional homogenization, to name a few examples), there is only one method that is capable of lysing all cell types (including bacterial ones) and that is high-pressure homogenization. Specifically, our high-pressure homogenizers here at BEE International incorporate a patented Emulsifying Cell (EC) technology that combines shear, cavitation and impact forces so that the high-pressure homogenizing process always renders optimal results. Whether used in research, pilot or industrial settings, BEEI high-pressure homogenizers ensure the best processes for bacterial cell lysis because they:

  • Provide accessibility of intracellular proteins for extraction and solubilization.
  • Disrupt all cell types since users can alter pressure, flow and processing time, as well as the amount of cavitation, shear and impact forces, to get the best results for the type of cell they are disrupting.
  • Offer flexibility, allowing easy adjustment for different cell disruption strategies.
  • Deliver high yields in less time, which lowers Total Cost of Operation (TCO).
  • Possess scalable technology, which enables users to elevate from laboratory scale to pilot scale, while achieving reproducible results.
  • Support various sample sizes and permit users to adjust the widest variety of mixing forces.
  • Require minimal training and are easy to use, reconfigure, clean and maintain.

 

Want to Learn More?

To learn more about high-pressure homogenization and why it is the best method for bacterial cell lysis, please contact our team at BEE International. We believe our equipment offers superior quality and provides the best value for researchers and manufacturers alike. Our proprietary processes guarantee that our customers produce high cell rupture yields in the shortest amount of time across the widest variety of cell types. 

You might also wish to download our free eBook, “7 Key Factors to Consider When Choosing a Cell Lysis Method,” for additional information bacterial cell lysis.

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Why We Love High Pressure Homogenizers (And You Should, Too!)

Posted by David Shechter on Aug 30, 2018 11:30:00 AM

Love High Pressure HomogenizersIf you were asked about some of your favorite products, a high-pressure homogenizer may not make the list. However, as we’ve discussed in a previous blog, the homogenization process produces a lot of products you most likely use and consume in your daily life. That’s one of the reasons we here at BEE International love homogenizers…but there are more! Let’s take a look at some of the most popular uses of high-pressure homogenization and the benefits they offer consumers.

Particle Size Reduction

High-pressure homogenization utilizes mechanical force to break down particles or droplets in a liquid into smaller and more uniform sizes, resulting in a dispersion, which is where fine particles of one substance are scattered throughout another substance. Dispersions can be further classified into solutions, suspensions and colloids, a mixture somewhere between a solution and a suspension.

Some high-pressure homogenizers can reduce particle size to as small as a single-digit nanometer. Plus, they ensure the composition is equal throughout, and the overall molecular makeup of the sample isn’t compromised. Many high-pressure homogenizers are able to produce emulsions, dispersions and suspensions at particle sizes of 100 µm or higher, and a select few can achieve a size of down to 0.1 µm. Key benefits of particle size reduction include:

  • Increased dissolution rate.

  • Improved drug delivery.

  • Controlled rate of reaction.

  • Increased density.

  • Consistent texture and product appearance.

  • Enhanced sedimentation stability.

  • Improved mineral processing.


Important Applications

Food and Beverages

Though one of the most well-known uses of high-pressure homogenization in the food and beverage industry is for milk, it is also widely used in the manufacture of sauces, spreads, salad dressings, fruit juices and concentrates, fish and meat pâté, sweeteners, baby food, egg product, other dairy products and more. It helps companies utilize to efficiently, cost-effectively and safely produce their goods. Food homogenizers also aid in the delivery of a product with uniform texture, flavoring and taste through a scalable process that allows for a longer period of freshness than food comprised of larger particles.

Pharmaceuticals, Chemicals and Biofuels

High-pressure homogenizers are used for numerous products from the chemical and biotechnology industries and products such as pharmaceutical tablet coatings, vaccinations and creams. They can be used to create cost effective medicines, modify microorganisms, synthesize new chemicals and develop alternative biofuels.

Cosmetic Creams and Lotions

Due to its ability to provide potent particle size reduction, enriched texture and consistency and strengthened emulsion stability, high-pressure homogenization is a preferred method for improving the production of cosmetic creams, lotions and other cosmetic products. It is especially beneficial for beauty creams and moisturizing lotions because they have to have a combination of the right texture and consistency in order for the skin to be able to effectively absorb them.

RNA Extraction

 Utilizing homogenization for RNA extraction enables processing to be effectively and repetitively processed in only seconds, thereby eliminating heat production. Its force and high pressure produce consistent and uniform samples, and it can process both small and large samples. Plus, homogenizers are scalable and compatible with many sample types, including those with tough cell walls. Using a powerful chaotropic salt solution, RNA extraction purifies RNA from biological samples by quickly disrupting cells or tissue to release the nucleic acids from the cells. These released RNAs are valuable in providing details on which genes are expressed (used to make products), to what degree the genes are active, how they work to regulate each other via small, regulatory RNAs and which structural RNAs (i.e. rRNAs, mRNAs and tRNAs) are produced.

Oil Emulsification

 Due to the fact that water and oil are immiscible, powerful mixing is essential to conducting oil emulsification. Therefore, specialized machines such as high-pressure homogenizers are preferred because they not only impart high shear but also are able to reduce particle sizes much more efficiently than other blending and emulsification methods. They are used in a wide variety of research and scientific uses and by many industries that require the use of active ingredients because they reduce active ingredients to a small enough size to ensure a thorough dispersion throughout the medium produced. Additional benefits of high-pressure homogenization for oil emulsification include production of stable emulsions, improved product consistency, shelf-life, texture, color and flavor and the ability to overcome the resistance caused by two immiscible liquids.

BEE International: Our Love for Our Products Shows in the Way We Treat Our Customers

BEE International offers homogenizers that are both high-quality and reliable. Our line of equipment is suitable for virtually every industry and can work in just about any environment. Contact us today to learn how our products can maximize your homogenization processes.

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Liquid Mixers vs High Pressure Homogenizers: Which Is Right for You?

Posted by Deb Shechter on Dec 6, 2017 11:30:00 AM

liquid mixersIt’s not uncommon for terms or even buzzwords in various industries to be similar but have different applications. For example, in the healthcare industry, electronic medical and electronic health records may sound the same. However, electronic medical records (EMRs) are a digital version of the paper charts in the clinician’s office that contain the medical and treatment history of the patients in one practice, while electronic health records (EHRs) focus on the total health of the patient, provide a broader view on a patient’s care and are designed to share information with other healthcare providers.

Similarly, though the terms “liquid homogenization” and “high-pressure homogenization” appear comparable, each has its own characteristics and primary uses. Homogenization in general can be defined as the reduction of particle size of any given molecule and is a process utilized often in the pharmaceutical, biotech, cosmetic and pharmaceutical industries. Its benefits include more even particle distribution, improved texture and enhanced appearance and taste.

Liquid homogenization, achieved by using a liquid mixer, and high pressure homogenization offer similar outcomes yet use distinct processes. The mixing processes and mixer designs chosen are determined by the nature of the materials to be mixed. Essentially, the type of product you select for your laboratory depends on its downstream applications and the cell types being used. 

In this blog, we’ll describe the differences between liquid and high-pressure homogenization and list pros and cons of each to help you decide which one is best suited to your business needs. Whichever you choose, it’s important that it has the flexibility to meet the various needs of a laboratory. 

Liquid Homogenization

Widely used for cell disruption of cultured cells and small sample volumes, liquid homogenization requires a sample to manually be forced through a narrow space, thereby shearing membranes and decreasing particle size. There are three most common types of liquid mixers used: the Dounce homogenizer (used for gently lysing single cell suspensions), Potter-Elvehjem homogenizer (used with delicate tissues) and the French press (used for small sample volumes). In addition to being suitable for operation at higher temperatures and easy to maintain, liquid mixers offer enhanced repeatability and a lower power requirement. Nevertheless, the throughput from all three models is fairly low, and they may not provide enough scalability. 

High-pressure Homogenization 

Utilized for numerous products from the chemical and biotechnology industries and products such as pharmaceutical tablet coatings, vaccinations and creams, high-pressure homogenization uses multiple mechanical forces (such as turbulence, cavitation and shear) along with intensive pressure to achieve micronization and membrane lysis. Similar to liquid mixers, high-pressure homogenizers work by forcing cell suspensions through a very narrow channel under pressure. Unlike the manual requirement of liquid homogenizers, though, high-pressure units run automatically and are often made to be scaled from laboratory experimentation to manufacturing.

Benefits of high-pressure homogenization include an increased rate of dissolution, ease of operation, cost and time effectiveness, consistency in composition of products, greater stability of final products due to a stable emulsion and upgraded scalability and repeatability. High-pressure homogenization also has the ability to reduce particle size to levels that are not achievable by most other mixing processes. Because they tend to be large and very heavy, high-pressure homogenizers are typically used for large volume processing and consume more power than liquid mixers. They sometimes require more complex cleaning, cannot handle large particles or high-fiber materials and aren’t the most suitable for high temperature materials. 

BEE: The Industry Leader in High-Pressure Homogenizers

BEE International offers a wide variety of high pressure homogenizers to meet the needs of virtually any industry. Unlike liquid mixers, our homogenizers are suited for large samples and offer large throughputs, making them ideal for laboratory, pilot plant and even industrial use. In addition, our easy-to-use equipment produces higher yield in less time and utilizes multiple forces – shear, turbulence, impact and cavitation – to allow for results that are reliable, repeatable and high quality.

There are plenty of companies on the market to select equipment from; however, the product can be of higher quality and more even consistency when run through top-shelf equipment, most frequently in the form of a homogenizer. BEE International Technologies is trusted by researchers around the world for both our laboratory homogenizers and associated customer support. Learn about how BEE’s products can maximize your homogenization processes by contacting us today. 

For more information on how to choose the right cell lysis method for your application, download our FREE eBook:

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4 Benefits of High Pressure Homogenization

Posted by Deb Shechter on Mar 30, 2017 12:30:00 PM

benefits of high pressure homogenizationHomogenization is an incredibly useful technique that is becoming more heavily utilized in a number of manufacturing industries, including pharmaceutical, biotech, cosmetic, and food. Homogenization is an important step in creating high-quality products that consumers have grown to expect. With benefits including improved taste, texture, and consistency of products – when then translate to increased sales and customer satisfaction – it’s no wonder that more and more industries are clamoring to get a homogenizer of their own! Continue reading to learn about more benefits of high pressure homogenization.

Consistency in Composition of Products

High pressure homogenization allows for an even dispersion of ingredients within a medium; this is incredibly important for the pharmaceutical industry, where active ingredients need to be evenly dispersed within a pill, tablet, capsule, or syrup – and within the cosmetic industry, where customers expect their lotions and creams to have an even texture and appearance.

Increased Rate of Dissolution

Homogenization involves pushing materials through a shaft to create smaller particles; this is especially important in the world of dissolution, where smaller and finer particles facilitate easier dissolution. In the pharmaceutical industry, this impacts both the bioavailability and efficacy of drugs that are produced.

Greater Stability of Final Products

A successfully homogenized product contains particles that are so small and fine that they do not fall out of solution. This is incredibly important to the food and beverage industry – the last thing a customer wants is a bottle of juice that has particles visibly falling out of solution!

Decreased Cost

Another benefit of high pressure homogenization is its ability to not only enhance the safety of products – especially important both in the food and pharmaceutical industries – but to reduce overall costs as well. Effective high pressure homogenization often results in a reduced need for processing chemicals and thermal processing, and can also decrease the potential costs of product recalls.

BEE International: High Quality High Pressure Homogenizers

At BEE International, we take pride in our wide array of homogenizing equipment. We offer an extensive 1-year warranty on parts and labor, with unmatched customer service and training. Our homogenization equipment utilizes the forces of shear, impact, cavitation and turbulence to ensure the highest quality of emulsions, dispersions, and finished product. Our equipment is well-suited for laboratory, research and development, pilot plant, and industrial use. To learn more about our selection of homogenizers, please contact us today for more information.

For more information on particle size reduction and how to achieve efficient and consistent results, download our FREE eBook:

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Dounce Homogenizer vs. High Pressure Homogenizer

Posted by David Shechter on Mar 15, 2017 12:30:00 PM

dounce vs. high pressure homogenizerIn the growing field of biotechology, homogenization – also known as micronization or more simply, particle size reduction – is an important process step in the study of tissues, cells, and other living organisms. However, although homogenization seems like a relatively simple process, it is one wrought with many options, each having their own sets of pros and cons. In this article, we will discuss the differences between two common pieces of equipment used to break down particles in the field of biotechnology – the dounce homogenizer, and the high pressure homogenizer. What are the differences and similarities? What are the pros and cons of each type of equipment? And what does BEE International have to offer in the way of this homogenization equipment? Continue reading to learn the answers to these questions and much more.

Dounce Homogenizers

The Dounce Homogenizer was developed in 1954 by Alexander Dounce, an American biochemistry professor. During his life, he studied the isolation and purification of cellular organelles and enzymes, as well as the chemical basis of protein synthesis. His work on the isolation of organelles, particularly nuclei and mitochondria, led to his development of the Dounce Homogenizer. This homogenizer consists of a glass mortar and pestle that is used to manually grind tissues and cells through the use of shear. This method is strong enough to lyse the cells while leaving more delicate organelles intact. Dounce homogenizers typically use two different pestles, each having a different width. A pestle with a larger width fits more tightly within the shaft of the dounce for maximum friction and cell disruption, while the other smaller pestle is better suited for creating a homogenous sample. While the Dounce homogenizer is easy to clean and sterilize (thanks to its glass construction),  its manual process does not lend itself well for the homogenization of larger amounts of samples.

High Pressure Homogenizers

High pressure homogenizers, similar to dounce homogenizers, also utilize the force of shear. However, these homogenizers use other forces in addition to shear: turbulence, impact, and pressure. High pressure homogenizers, as the name suggest, utilize pressure to force cells through a small orifice, which then shears and lyses the cells in question. This act happens quickly and uniformly, which allows much larger quantities of material to be homogenized at once. High pressure homogenizers, much like Dounce homogenizers, also offer the benefit of easy cleaning and sterilization.

BEE International: Homogenizers for Any Job

At BEE International, we offer a number of high pressure homogenizers to tackle virtually any task in a laboratory, pilot plant, or industrial setting. Please contact us today to learn more about our offerings as well as more information on how we can assist you with your next homogenization project.  

For more information on homogenization and different cell lysis methods, download our FREE eBook:

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