What is the Purpose of Homogenization?

Posted by Deb Shechter on Nov 21, 2019 1:00:00 PM

purpose of homogenizationHomogenization is the process by which a sample is broken into identical parts so that removing one portion of it does not disrupt and still accurately reflects the remaining sample’s molecular composition. In many instances, it is also used to thoroughly mix together naturally immiscible substances; when a sample is reduced to tiny, uniform pieces, it is easier for manufacturers and scientists to combine it with another sample, creating many of the products used across a wide variety of industries.

Here’s a brief overview of the purpose of homogenization:

 

To Reduce Particle Size

First and foremost, the purpose of homogenization is to reduce the size of the particles making up a sample. In simple terms, it’s a bit like taking a boulder and smashing it into gravel, except, in this case, the gravel pieces would be all the same size and shape. During homogenization, many samples can be reduced to nanometer-sized particles. This makes it easier for manufacturers to produce things like adhesives, resins, paints and creams/lotions and guarantee that they work.

 

To Lyse Cells

For the molecular and cellular biology fields, one of the most common purposes of homogenization is to breach the cell wall and/or membrane and expose the intracellular contents of cells. This is necessary when scientists need to make biological samples for further study, experimentation and research.

 

To Kill Pathogens

One the the added benefits of many homogenization techniques is the destruction of pathogens within a sample. There are several kinds of homogenizers (mechanical, high pressure and ultrasonic), each using one or more specific types of force. As a by-product of the processing method each force facilitates, many homogenizers render samples with varying levels of sterility. For instance, high pressure homogenizers can effectively kill microorganisms in food samples. Other homogenizers that produce high levels of heat (most mechanical homogenizers) can kill various pathogens, as well, but the accompanying heat frequently degrades the overall sample. Thus, it’s important to pick the right kind of homogenizing equipment if product sterility is a requisite goal.

 

To Facilitate Stable Emulsions and Dispersions

Finally, the purpose of most homogenizers is to mix substances well. Reducing the size of the particles in a substance allows it to more readily and easily combine with the particles of another substance. It also allows all of the particles to remain mixed for a longer period of time. This is especially important for products that need to be consistent in appearance, taste, texture, viscosity and/or nutritional value for the length of their shelf lives, like cosmetics, food and beverage items and pharmaceuticals.

 

The BEE International Advantage

Homogenization offers a host of benefits, and we here at BEE International believe our high pressure homogenizers are the best choice, offering advantages that would otherwise necessitate the purchase of multiple types of equipment instead of just one! Our patented Emulsifying Cell (EC) technology, along with our proprietary combination of multiple homogenizing forces, mean our homogenizers have the ability to create more stable products with longer shelf lives in shorter amounts of time with fewer passes than our competition. Please contact us to capitalize on all the purposes of homogenization or if you're interested in the particle size reduction side of homogenization, download our free eBook now:

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Ultrasonic Homogenizer 101: The Basics

Posted by Deb Shechter on Jul 31, 2019 12:43:50 PM

Ultrasonic HomogenizerHomogenizers come in a variety of shapes and sizes and operate under several different kinds of forces. The ultrasonic homogenizer is a specific type of homogenizer which uses ultrasonic waves and cavitation to force apart and reduce the size of particles (both liquid and solid) within a liquid substance.

 

The What

Also known as sonicators, ultrasonic homogenizers consist of three parts:

  1. A generator, which supplies energy.
  2. A converter, which converts and increases electrical energy into mechanical motion.
  3. A horn, probe or tip, which when placed into a liquid moves in a rapid fashion and causes bubbles to form. These bubbles expand and contract, breaking apart the surrounding particles (called cavitation).

 

The Why

Of course, the goal of homogenization is not only to break apart, but to mix, particles. And in this sense, ultrasonic homogenizers hold an advantage, as their stimulation of cavitation so effectively disrupts molecular bonds that the total particle surface area is greatly increased. This is because breaking apart particles creates more particles with more surfaces. And when there is a greater total surface area and a tighter distribution of particles, there is improved opportunity for particle agglomeration and overall improved stability.

 

Furthermore, ultrasonic homogenizers have fewer parts to maintain and to clean, and they can be customized according to specific amplitude and pressure ranges, making them great choices for scientists and manufacturers wanting complete control over the cavitation process.

 

The When

Ultrasonic homogenizers can be used to homogenize samples, lyse cells, reduce particle size, extract biological material, refine chemical processes and more, but only with liquid (or mostly liquid) samples. They are appropriate for application in both laboratory and industrial settings and are especially valuable when a sample can’t be stirred, doesn’t require grinding or cutting for processing and won’t be degraded by heat (as high temperatures often result with the rapid agitation of the sonicator tip).

 

The Who

As mentioned, ultrasonic homogenizers can be used in a wide variety of industries by all kinds of professionals. Life scientists can use them to facilitate sonoporation. Jewelers and opticians can use them to clean their merchandise. Food scientists, agriculturalists and chemists can use them to enhance their "soft goods" processing. Archaeologists can use them to uncover microfossils. Pharmaceutical researchers can use them to produce betters drugs. And these are just a few examples!

 

Want to Learn More?

Picking the right kind of homogenizer depends on the type of materials with which you work and what you want to accomplish with them. If you need help selecting the right homogenizer for your task, contact our team at BEE International.

 

Please feel free to also download our complimentary eBook, “7 Key Factors to Consider When Choosing a Cell Lysis Method,” for practical advice on enhancing your time, budget and skills with the right piece of equipment.

 

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Homogenizer Basics: Everything You Need to Know

Posted by Jen Hug on Mar 28, 2019 11:01:00 AM

Homogenizer BasicsHomogenization is the process of breaking apart one substance (a gas, liquid or solid) and mixing it into another (usually liquid) one with which it normally wouldn’t combine. It’s not a particularly complicated concept, except when you consider the fact that it relies on one or more external sources of energy exerting the necessary force to break the substance(s) apart, often into nanoparticles. Many industries utilize homogenization to facilitate more stable mixtures with better bioavailability, controlled consistencies and longer shelf lives. The type of equipment they use to supply the force they need — called a homogenizer — directly depends on the type of substances being mixed, as well as the finished product’s intended use. Here’s a quick overview of everything you need to know about homogenizers on the market today:

Types of Homogenizers

There are many types of homogenizers, ranging from common kitchen implements like mortars and pestles and blenders to more sophisticated equipment like bead mills and cell lysers. They are classified according to the type of force they supply, with the most basic ones being:

  1. Mechanical Homogenizers - which make use of turbulent agitation, stirring, shearing and/or impact to effectuate the breakdown and mixture of substances. Specific examples of mechanical homogenizers include bead mills, rotor-stator homogenizers and blade homogenizers.

 

  1. High-Pressure Homogenizers (HPH) - which force pressurized cell suspensions through small tunnels or holes in order to disrupt their bonds. Many of these types of homogenizers also include some sort of high velocity impact of the product (in addition to pressure) to better ensure cell disruption.

 

  1. Ultrasonic Homogenizers - which combine extreme sonic pressure waves in varying types of vessels to form microbubbles that eventually implode (also called cavitation), breaking covalent bonds and prompting the blending process.

 

How Homogenizers are Used

As discussed, homogenizers are used in a variety of industries for a variety of purposes. Some of the most common ones are:

  1. Food and Beverage - Food and beverage manufacturers rely on homogenizers to improve the stability and appearance of their products. The homogenization process keeps milk from separating into cream and other liquid and viscous substances (like orange juice, milk, mayonnaise, salad dressings and yogurt) from dividing back to their individual parts.

 

  1. Biotech Industry - The biotech industry includes those companies that make products out of living organisms. These types of businesses, mostly concentrated in the medical and agricultural fields, work with microorganisms (like bacteria, fungi and viruses), as well as plant and animal tissues, to modify crops and livestock, create and test medicines and engineer biofuels. They use homogenizers to lyse cells, reduce particle size and create micro and nano particles for both research and production objectives. Specially, homogenizers help ensure that biotech processes consistently maintain scalable results while always preserving the integrity of cell contents.

 

  1. Pharmaceutical and Chemical Industries - Whether they use chemicals to manufacture drugs, plastics or other products, both pharmaceutical and industrial chemical companies need homogenizers to help them break apart one substance so that it can be evenly dispersed into another one. Not only does this facilitate easier and more uniform mixing, it creates a final product with “a tighter distribution of smaller particles." This increases the bioavailability and lengthens the shelf life of pharmaceutical products, and it increases conductivity and improves surface cohesion of products used for industrial chemical applications. Indeed, chemical products of all kinds, from life-saving medications to paint, need homogenizers to effectively blend product particles, sustain uniformity and cut down costs.

 

The BEE International Difference

Most homogenizers utilize only one type of force, but BEE International homogenizers combine high pressure with turbulence, cavitation, impact and shear forces to maximize the time, energy and money of the companies who use them. Our patented Emulsifying Cell (EC) technology guarantees scalability of results with customizable options that can be effectively programmed for hundreds of applications. Contact us to learn how our proprietary processes can improve your products, as well as your bottom line.

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How to Improve Oil Emulsification with a Homogenizer

Posted by Deb Shechter on Jan 31, 2019 11:00:00 AM

oil emulsification with homogenizersEmulsification is the process by which two naturally immiscible liquids are forcefully mixed together. Salad vinaigrettes, mayonnaise, butter, cold creams, lip balms and medicated ointments are all examples of emulsions. Depending on the ratio of oil to water, emulsions are typically categorized as either oil suspended in water (o/w) or water suspended in oil (w/o). Using the right emulsifying agent and the right kind of force, you can compel either type of mixture, effectively manufacturing many textile, plastic and paper products and improving the stability, bioavailability and viability for a variety of food, cosmetic and pharmaceutical ones (among others). Take a look at how a high pressure homogenizer can improve the oil emulsification process in particular, giving you better products in less time:

But First…The Difference Between an Emulsifier and a Homogenizer

Both emulsifiers and homogenizers forcefully mix many substances. But while an emulsifier only makes use of one type of mechanical force, a homogenizer makes use of many. At BEE International, our proprietary homogenizing system combines turbulent premixing with product progression through highly pressurized spaces, utilizing shear, cavitation and impact to break apart particles and uniformly mix and blend them. The result is a product with multiple benefits.

The Benefits of Using a Homogenizer in the Oil Emulsification Process

The benefits of using a homogenizer to create emulsified products are two-fold:

  1. It Reduces Particle Size

The high pressure and additional forces used by high pressure homogenizing systems (like those with our patented BEE International Emulsifying Cell technology) drastically reduce product particle size. Sub-micro particles have more surface area per particle, which means each has more opportunity to interact with another. This not only makes it easier for mixing agents to do their job (after all, they have a larger area on which to apply their “glue”), it also creates a final product with identical (i.e., homogenous) properties, improving the texture, consistency and appearance of many consumables, (especially important for those in the food and cosmetic markets) and increasing the bioavailability of drugs for the pharmaceutical industry.

  1. It Efficiently and Effectively Mixes Resistant Substances

The vigorous and multiple forces utilized by homogenizers make it harder for product particles to resist initial mixing and harder for them to separate afterwards. It also allows for large volume processing since homogenizers have greater capacities than traditional emulsifiers. The ability to create more stable products with longer shelf lives in shorter amounts of time with fewer passes saves time and money, valuable benefits for companies wanting to make the most of their resources.

Ready To Learn More?

The value of a high pressure homogenizer extends far beyond mere mixing of substances, making it the equipment of choice for companies wanting to maximize their processes while still achieving a superior meld. If you need assistance determining if or how homogenizers can improve your manufacturing turnover, as well as provide you with superior oil emulsifications, contact our team at BEE International. Our HPP homogenizers aid all types of businesses, including those in the food, pharmaceutical, chemical, biofuel and cosmetic industries and more. 

Feel free to also download our free eBook, "How to Achieve Efficient & Consistent Particle Size Reduction," for additional information about the value of smaller particle size within a manufacturing context.

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What Are Homogenizing Agents & How Do They Improve the Homogenization Process?

Posted by Deb Shechter on Dec 17, 2018 12:10:59 PM

homogenizing agentsFor most people, “homogenization” and “homogenizing agents” aren’t words that just roll off the tongue. But for us here at BEE International, talking about them comes as easily as discussing our favorite TV show or the day’s weather. So, if you’ve ever wondered what “homogenized milk” meant or how your face cream was made or why some peanut butter doesn’t have to be stirred, we’d love to share with you the facts about high pressure homogenization and homogenizing agents. Consider the following:

An Overview of the Homogenization Process

Homogenization is the process of combining two substances by breaking them into uniform pieces and mixing them together evenly. Homogenization facilitates many laboratory and manufacturing processes, such as those in the pharmaceutical, biotech, chemical, cosmetic and food industries. Not only does homogenization change the physical characteristics of materials, making it easier for scientists and manufacturers to work with raw components, it also helps ensure product quality, maintain long-term storage of materials and isolate certain particles when necessary. All of these things are necessary for superior performance among a variety of products. Homogenization makes our milk taste good and keeps our peanut butter from separating into a lump of peanut paste and a pool of oil. It keeps our lotions thick, yet spreadable, and our medicines effective.

But what if certain materials resist homogenization attempts by mechanical equipment alone? What if they need something more to help break them apart and realign their parts equally? In these cases, homogenizing agents are used.

What are Homogenizing Agents?

Homogenizing agents are resin-based mixtures which are usually added at the beginning of a homogenization cycle. When heated to certain temperatures, they turn “sticky” and help to moisten and soften the surfaces of the substances being melded together. 

How Do They Improve the Homogenization Process?

Because of their tackiness, homogenizing agents make it easier for some substances to blend and distribute their particles evenly. They also encourage filler acceptance. And by helping to shorten the time it takes to blend materials regardless of their “molecular weight, viscosity or polarity,” homogenizing agents save energy and other valuable resources.

In scientific and research settings, efficient homogenization can do more than just save time, though. It can also save lives. The right homogenizer and the right homogenizing agents can reduce the number of passes it takes to get viable results, ensuring that all steps in the testing cycle are consistent and go as smoothly and quickly as possible.

In more industrial settings, the right homogenizers and homogenizing agents ensure that applications are customized according to overall purposes, whether that be securing product stability; guaranteeing sterility; improving color, texture, taste and nutritional value; or simply maintaining cost effectiveness. 

Need More Info?

If you need help selecting the right homogenizer for your homogenization process, contact us today. Our high pressure homogenizers are customized to suit your own unique needs with multiple mechanical forces that create thoroughly mixed products.

And for additional information, you might also like to download our FREE eBook: "How to Achieve Efficient & Consistent Particle Size Reduction." In it, we show how equipment that reduces particle size saves you money by extending shelf life and strengthening biophysical properties. 

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The Ultimate Cheat Sheet for Choosing a Cell Homogenizer

Posted by David Shechter on Aug 16, 2018 11:30:00 AM

Businessman is standing in front of two direction signs.If you attended high school before laptops and tablet computers replaced books and used a landline to make phone calls, you’ve probably heard of CliffsNotes. Often used to help students figure out the intricacies of a novel that was required reading for English class, these handy pamphlets were a sort of a study guide highlighting the work’s key points.

In this blog, we’re offering you a CliffNotes version of tips for selecting a cell homogenizer that best meets your business needs. We’ll outline cell homogenization, often referred to as cell lysis, offer key factors for how best to achieve it and provide some benefits on it.

What is Cell Lysis?

Cell lysis, which takes place in biotech, pharmaceutical, food, cosmetic and chemical laboratories on a regular basis, is the rupture of the cell membrane resulting in the release of cell contents and the subsequent death of the cell. Research scientists and laboratory technicians disrupt the cell’s structure to extract organelles, proteins, DNA or mRNA. 

Cell lysis can be achieved through mechanical or chemical methods. Mechanical cell lysis techniques include high shear mixing, traditional homogenization, sonication, grinding, freeze/thaw cycles and high-pressure homogenization. Chemical cell lysis techniques include osmotic lysis and the use of detergents, chelating agents or chaotropic agents.

In order for cell lysis to be performed successfully, the following factors should be present:

  • Intracellular proteins should be accessible for extraction and solubilization.
  • The process should be flexible, as different cells require different cell lysis strategies.
  • The technique should result in a high yield in a short time.
  • The process should be relatively easy to perform.
  • Results should be consistently reproducible and scalable.

 

How Homogenization Helps

Of the methods used to achieve cell lysis, the most widely used is homogenization, which offers a multitude of benefits for production laboratories and the consumers who buy their products. It is especially useful for small volumes of cells and/or cultured ones and involves the use of shearing force on the cell. By forcing a small cell through an even smaller-sized orifice, homogenization removes the outer layer and lyses the cell. Some cell lysis products use utilize shear, and others employ sonic cavitation. However, the best choice when purchasing a homogenizer for cell lysis is one that uses shear, cavitation and impact and lets the user adjust the relative amount of each force for different applications.

The homogenizer you choose for cell lysis should be scalable to allow for customization in pressure and intensity, resulting in both cost and time efficiency for the user. It also must accommodate for multiple cell types and adjust for various sample sizes. Other factors to consider when selecting the right cell homogenizer are the force required to produce the highest yield in the least amount of time and the volume, efficiency, stability and sanitary standards compliance of the product. Advantages of using this type of homogenizer are:

  • Accessibility of intracellular proteins.
  • Built-in process flexibility.
  • Ease-of-use and low learning curve.
  • Reproducible and scalable results.
  • Various sample size processing.
  • Ability to disrupt all cell types.
  • Better results in fewer passes.

BEEI International: Bringing the Best Homogenizers to You

BEE International offers numerous high-quality homogenizers to meet the needs of any industry for cell lysis. Our laboratory-grade models include the Nano DeBEEMicro DeBEE and Mini DeBEE, all of which are easy to use, provide consistent and reliable results and easily scale up for full scale production. Plus, they offer maximum experimentation capabilities and the ability to change flow, pressure, shear and impact settings. Contact us today to learn more about our full line of homogenizers.

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Summer, Sunscreen, and Homogenization

Posted by David Shechter on Jun 13, 2018 11:30:00 AM

bee1For many people across the United States, the winter of 2017 was one of the coldest on record. Residents of the East Coast had to deal with multiple Nor'easters, shutting down airports and wreaking havoc on roadways.

For most of us, summer and the high temperatures that typically accompany it are definitely welcome. Kids are out of school, baseball games are being played and swimming pools are being opened and prepped for lazy summer days.

With the arrival of summer, though, comes a reminder about the dangers of getting too much sun. More people are diagnosed with skin cancer annually in the U.S. than all other cancers combined, and the annual cost for treating skin cancers in this country is an estimated $8.1 billion.

Skin cancer may be prevented or at least minimized through the regular use of sunscreen. In fact, scientific evidence supports the benefits of using sunscreen to reduce short- and long-term damage to the skin from the sun’s rays.  

High-pressure homogenization is a preferred method for improving the production of cosmetic creams or lotions, including sunscreen, because it offers the following benefits:

Potent Particle Size Reduction

By forcing a sample through a narrow space while applying high pressure and multiple mechanical forces, including cavitation, shear, impact and turbulence, high-pressure homogenization more effectively decreases particle size. This reduction, or micronization, creates a more homogenous mixture, provides better active ingredient dispersion and stabilizes product emulsions. It’s especially beneficial for creams, lotions and other cosmetic products because of the positive effect on the quality of these items. 

Enriched Texture and Consistency

High-pressure homogenization is often preferred over other methods because of it’s capability to create a product that is uniformly mixed. This leads to smoother texture and enhanced consistency, which is essential for beauty creams and moisturizing lotions because they have to have a combination of the right texture and consistency in order for the skin to be able to effectively absorb them. 

Strengthened Emulsion Stability

Semi-solid products such as lotions and creams are comprised of emulsions, which are a mixture of two immiscible liquids. Micronization of droplets acts to eliminate the separation of water phase of homogenization, resulting in a more permanent mixture of oil and water. This permanence extends the shelf life of these semi-solid products and improves their appearance through consistent coloring.

BEEI: Homogenizers for High-quality Creams and Lotions

If you’re searching for a cosmetic homogenizer to meet your laboratory and/or production needs, make sure you choose one that’s high quality and includes a combination of capabilities. Whether your product is a cream, lotion, makeup or personal care item, opting for a customizable high-pressure homogenizer will only improve and enhance its effectiveness.

Contact us today to learn more about our full line of homogenizers and what they can do for you.

For more information on homogenization and how to achieve efficient and consistent particle size reduction, download our FREE eBook:

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Homogenization and the Industries That Use It

Posted by Tal Shechter on May 29, 2018 2:00:00 PM

bee2Many people go to a local store to buy food, personal care products, medicine and other items. However, most of us don’t spend any time considering what went into the production of these items. In many cases, it likely involved homogenization.

Homogenization is a process utilized for particle size reduction. It works by forcing a sample through a narrow space, and multiple forces, including turbulence and cavitation in addition to high pressure, can act on the sample to create a high-quality product. It can be used on many different types of material, such as plant, food, soil, tissue and more. Plus, it provides benefits for multiple applications, such as high-pressure pasteurization, dispersions, micro/nano emulsions and cell disruption.

Although many other types mixing machines exist, homogenizers are scalable, and their use of multiple mechanical forces instead of just one results in stable, uniform and consistent products. They are used in a wide variety of research and scientific uses and by many industries that require the use of active ingredients because they reduce active ingredients to a small enough size to ensure a thorough dispersion throughout the medium produced.

Pharmaceutical

Powerful mixing and particle size reduction are key techniques among pharmaceutical products. In the pharmaceutical industry, researchers use homogenizers during laboratory research and in clinical trials and manufacturing for products including vaccines, antibiotics, cancer treatment and medicinal creams. Benefits of homogenization for pharmaceutical products include improve delivery of active pharmaceutical ingredients, increased digestibility, lengthened shelf life and improved reliability in manufacturing settings.

The most important uses for homogenizers in the pharmaceutical industry are particle size reduction, scaling for drug development and synthesis of essential drug components. Though other mixing methods exist, homogenization is the most scalable, resulting in increased cost savings, lower time requirements and a decrease in the likelihood of failure for the product for which its used.

Food and Beverage

Many people identify the use of homogenization in the food and beverage industry with milk; it gives milk a longer shelf life and makes it more stable and visually pleasing. Homogenization is primarily used in the food and beverage industry to help manufacturers improve the composition and quality of their products and ensure their end products meet standards for visual appearance, texture and taste. 

Because homogenization utilizes pressure instead of heat, valuable nutrient content and flavor are preserved, chemical structures are stabilized and food processing outcomes are improved. It is a preferred method for producing emulsions, suspensions and other products that require a liquid suspended in another liquid or a small particle size. These products include mayonnaise, ice cream, cream liqueurs, salad dressings, dairy products and more.

Biotech

The biotech industry is comprised of the fields of cellular biology and molecular biology. Homogenizers are most commonly used in this industry to achieve effective cell rupture, enhanced particle size reduction and nanoparticle production. Although they decrease particle size, they ensure that a composition is equal throughout without compromising the overall molecular makeup of the sample.

Homogenizers also can be used to create cost effective medicines, modify microorganisms, synthesize new chemicals and develop alternative biofuels. In the case of cell disruption, they allow for a variety of cells, including E. coli, yeast, bacteria and delicate animal tissues, to be ruptured to recover the contents of essential cells.

BEE International: First on the Road to Reliability

BEE International offers homogenizers that are both high-quality and reliable. Our line of equipment is suitable for virtually every industry and can work in just about any environment. Contact us today to learn how our products can maximize your homogenization processes.

To learn more about 7 key factors to consider when choosing a cell lysis method, no matter the industry you're in, download our FREE eBook:

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Top Tips for Selecting a Tissue Homogenizer

Posted by David Shechter on Feb 27, 2018 2:30:00 PM

tissue homogenizerThe first thing many consumers point to when they hear the term “homogenization” is milk. August Gaulin received a patent for his homogenizer in 1899 and exhibited it to “treat” milk in 1900 at the World’s Fair in Paris.

Homogenization works by forcing the sample through a narrow space, and multiple forces  –– including turbulence and cavitation in addition to high pressure –– can act on the sample to create a high quality product. It can be used on many different types of material, such as plant, food, soil, tissue and more. In this blog, we’ll focus on tissue homogenization and what to consider when selecting a homogenizer to fit your specific business needs. 

In summary, tissue homogenization is a process employed to prepare tissue samples for further study, research or development. It involves the lysing (breaking apart) of cells to release their contents, from proteins and small molecules to DNA and RNA and more. The type of cell being lysed often dictates the homogenization device or technique that is used to complete the task. Homogenization is not only usually the simplest and safest approach to preparing such contents, it also is one of the quickest and most cost-effective methods. 

Techniques for Tissue Homogenization 

Knowing what techniques are best utilized for tissue homogenization can make choosing the right product a much easier proposition. These four techniques include chemical homogenization, freeze-thawing, and mechanical and ultrasonic homogenization. 

Chemical homogenization is best for small samples because the cost of materials used can be high for industrial-sized products. Freeze-thawing requires multiple cycles and a lot of time, and ultrasonic homogenization is only appropriate for tissues and molecules that aren’t affected by the temperature increase resulting from the high amount of heat it generates. The mechanical homogenization method can easily be scaled and offers time-efficient and consistent results. Overall, mechanical and ultrasonic homogenization are the two most commonly-used techniques.

High pressure homogenization allows for the forces of turbulence, cavitation, shear and impact to be used simultaneously to produce the best end result, even with delicate tissues. However, the lysate can be of higher quality and more even consistency when run through top-shelf equipment. 

Following is a list of things to consider when selecting a tissue homogenizer:

  • Toughness of tissue - Not all homogenizers can process more fibrous tissue, and those that can typically require a lengthy processing time.
  • Sample size and type – Large samples usually take more time to homogenize. Animal and plant tissues, yeast and bacteria typically require more rigorous methods of disruption.
  • Available components – Consider the product’s motor size, speed control and range, weight and dimensions and processing range.
  • Sample safety – The homogenizer used should be able to rapidly release the protein from its intracellular compartment into a buffer that isn’t harmful to the biological activity of the protein of interest. 
  • Uniformity of samples – The selected homogenizer should have the capability to quickly and safely produce consistent samples.


Before making a final decision on a homogenizer purchase, it’s a good idea to consult with businesses and/or laboratories with needs similar to yours. Another good idea is to request a demonstration from a couple manufacturers and compare the speed, cost and automation capabilities of the products

BEE International: The Top Choice for Tissue Homogenization

BEE International offers a number of high quality, high pressure homogenizers to achieve your goals. With our homogenizers, you will reap the benefits of our process, which includes:

  • Tighter distribution of smaller particles
  • Maximum particle size reduction in fewer passes
  • Increased manufacturing efficiency and reduced cost
To learn more about our line of homogenizers and how we can help you achieve your business goals, please contact us today. If you're looking for more information on how to lyse tissue cells, download our FREE eBook:

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How Does RNA Extraction Homogenization Work? 

Posted by Deb Shechter on Jan 25, 2018 11:30:00 AM

RNA extraction homogenizationMany people are familiar with the term “DNA,” which is the hereditary material in humans and almost all other organisms. Officially named deoxyribonucleic acid, it a molecule that carries the genetic instructions used in the growth, development, functioning and reproduction of all known living organisms and many viruses.

Not as well known outside the field of science is RNA or ribonucleic acid. RNA, a type of nucleic acid containing only one strand (DNA is primarily double-stranded), provides the link between the genetic information through protein synthesis. Procuring high-grade RNA, which is accomplished through a process called RNA extraction (or isolation), is the first step for a multitude of molecular techniques often utilized in gene expression including microarray analysis, real-time polymerase chain reaction (PCR), digital PCR, northern analysis and cDNA library construction.

RNA Extraction

Using a powerful chaotropic salt solution, RNA extraction purifies RNA from biological samples by quickly disrupting cells or tissue to release the nucleic acids from the cells. These released RNAs are valuable in providing details on which genes are expressed (used to make products), to what degree the genes are active, how they work to regulate each other via small, regulatory RNAs and which structural RNAs (i.e. rRNAs, mRNAs and tRNAs) are produced.

RNA extraction can be very challenging due to the difficulty of isolating RNA that is intact. This is in large part because of ribonuclease (RNase) enzymes found in cells, enzymes which are copious in the environment and can swiftly degrade RNA. Therefore, it’s vital that it be done cautiously and in a timely manner.

Homogenization – Organic RNA Extraction

The disruption of cell and tissue samples, often achieved by homogenization, is a required part of extracting RNAs and preventing their degradation. In fact, samples aren’t guarded from RNA degradation unless they are entirely homogenized. Although homogenization is especially functional for use with soft tissues, the method used should be based on the sort of cell or tissue. For instance, plant and animal tissues, yeast and bacteria typically require more exacting disruption methods. Either way, the sample or tissue should be kept frozen until it is ready to be homogenized.

Utilizing homogenization for RNA extraction is especially beneficial because it enables processing to be effectively and repetitively processed in only seconds, thereby eliminating heat production. Its force and high pressure produce consistent and uniform samples, and it can process both small and large samples. Plus, homogenizers are scalable and compatible with many sample types, including those with tough cell walls.

BEE International – Offering Simple Solutions for Complex Business Needs

We offer a number of top-of-the-line high-pressure homogenizers to achieve your business goals, and our scalable equipment is easy to use and produces better results in fewer passes. In addition, we’re globally recognized by laboratory managers and researchers for our high-quality products and excellent customer support. Contact us today to discuss your homogenization needs.

For more information on how to achieve efficient and consistent particle size reduction, download our FREE eBook:

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