How Does RNA Extraction Homogenization Work? 

Posted by Deb Shechter on Jan 25, 2018 11:30:00 AM

RNA extraction homogenizationMany people are familiar with the term “DNA,” which is the hereditary material in humans and almost all other organisms. Officially named deoxyribonucleic acid, it a molecule that carries the genetic instructions used in the growth, development, functioning and reproduction of all known living organisms and many viruses.

Not as well known outside the field of science is RNA or ribonucleic acid. RNA, a type of nucleic acid containing only one strand (DNA is primarily double-stranded), provides the link between the genetic information through protein synthesis. Procuring high-grade RNA, which is accomplished through a process called RNA extraction (or isolation), is the first step for a multitude of molecular techniques often utilized in gene expression including microarray analysis, real-time polymerase chain reaction (PCR), digital PCR, northern analysis and cDNA library construction.

RNA Extraction

Using a powerful chaotropic salt solution, RNA extraction purifies RNA from biological samples by quickly disrupting cells or tissue to release the nucleic acids from the cells. These released RNAs are valuable in providing details on which genes are expressed (used to make products), to what degree the genes are active, how they work to regulate each other via small, regulatory RNAs and which structural RNAs (i.e. rRNAs, mRNAs and tRNAs) are produced.

RNA extraction can be very challenging due to the difficulty of isolating RNA that is intact. This is in large part because of ribonuclease (RNase) enzymes found in cells, enzymes which are copious in the environment and can swiftly degrade RNA. Therefore, it’s vital that it be done cautiously and in a timely manner.

Homogenization – Organic RNA Extraction

The disruption of cell and tissue samples, often achieved by homogenization, is a required part of extracting RNAs and preventing their degradation. In fact, samples aren’t guarded from RNA degradation unless they are entirely homogenized. Although homogenization is especially functional for use with soft tissues, the method used should be based on the sort of cell or tissue. For instance, plant and animal tissues, yeast and bacteria typically require more exacting disruption methods. Either way, the sample or tissue should be kept frozen until it is ready to be homogenized.

Utilizing homogenization for RNA extraction is especially beneficial because it enables processing to be effectively and repetitively processed in only seconds, thereby eliminating heat production. Its force and high pressure produce consistent and uniform samples, and it can process both small and large samples. Plus, homogenizers are scalable and compatible with many sample types, including those with tough cell walls.

BEE International – Offering Simple Solutions for Complex Business Needs

We offer a number of top-of-the-line high-pressure homogenizers to achieve your business goals, and our scalable equipment is easy to use and produces better results in fewer passes. In addition, we’re globally recognized by laboratory managers and researchers for our high-quality products and excellent customer support. Contact us today to discuss your homogenization needs.

For more information on how to achieve efficient and consistent particle size reduction, download our FREE eBook:

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Chemical Methods for Cell Disruption

Posted by David Shechter on Jan 16, 2018 11:30:00 AM

cell disruptionCell disruption is used throughout many industries, from cosmetics and pharmaceuticals to food and biotechnology. As a method or process employed to release biological molecules or other materials from inside a cell, these industries commonly utilize it to study intercellular materials or use the materials located inside a given cell. It can be achieved through a range of methods and technologies, either mechanical or non-mechanical.

The method of cell disruption selected depends on the product, scale and cell type and must obtain the necessary components effectively and reliably without disturbing any of its components. Though high-pressure homogenization is the most common procedure for cell disruption, chemical methods including osmotic lysis, surfactants and chaotropic agents also are prevalent. In this blog, we’ll go into more detail on each of these methods.

Osmotic Lysis

In osmotic lysis (or shock), cells are suspended in a hypotonic extracellular environment, usually a dilute sucrose solution, causing them to take on water, swell and burst. It’s often applied to lyse mammalian blood cells and for RNA extraction, although it is sometimes used to disrupt microbial cells. Due to its low efficiency, however, it is one of the less commonly used chemical methods of cell disruption.

Surfactants

Surfactants (surface acting agents), often called detergents, are compounds that lower surface tension and disrupt the distinct interface between hydrophobic and hydrophilic systems. Their hydrophilic head and hydrophobic tail enable them to insert into and then disperse biological membranes. They are used at fairly low concentrations, and in order to disrupt bacterial cells, they must be used with lysozyme.

Detergents used for disrupting cells are divided three categories depending on their electrical charge: anionic, cationic and non-ionic. All three directly damage the cell wall or membrane, although the best detergents can lyse cells and solubilize proteins. Surfactant cell lysis may be used with ultrasonic processing in order to facilitate disruption.

Surfactants aren’t used for cell disruption more often because they denature proteins in the process and have different impacts on biological systems depending on which detergent is utilized and what its concentration is. No one detergent can be employed for all applications, and some of those selected may disturb downstream processing steps further in the process.

Chaotropic Agents

Chaotropic agents such as urea, guanidine and sodium iodide are capable of bringing some hydrophobic compounds into aqueous solutions by disrupting the structure of water, making it a less hydrophilic environment, and weakening the hydrophobic interactions among solute molecules. Used in very high concentrations, they are like surfactants because they break non-covalent interactions. Unlike surfactants, though, chaotropic agents disrupt the weak interactions between molecules and are sometimes used with detergents to denature and emulsify biological systems.

These techniques are usually only viable at laboratory scale due to increased consumption of energy, chemicals and water and can be very costly for use in large-scale manufacturing. Conditions during the chemical disruption process aren’t always uniform among samples, making it a risky proposition. In addition, some components may cause the denaturation of protein, resulting in a damaged end product.

High-pressure Homogenization

In high-pressure homogenization, a cell disruption method most often used for soft, solid tissues, samples are forced through a narrow space while pressure is applied to them. As the temperature increases, so does the rate of cell disruption. Although they can be used for small batches, high-pressure homogenizers are scalable and can easily adapt to different sample sizes to accommodate increased demand. They not only provide a high level of disruption efficiency but also can be used for the recovery of recombinant proteins.

BEE International: Meeting the Need for Mechanical Cell Disruption

At BEE International, our high pressure homogenizing technology allows you to gently rupture cells without damaging their valuable intracellular materials. You can control the pressure, enabling rupture of a variety of cell types. No harsh chemicals are introduced into the process, and all results are 100 percent scalable to meet your manufacturing needs.

To learn more about cell disruption and how our line of high-pressure homogenizers can help you achieve your goals, please contact us today.

For more information on cell lysis methods and key factors you need to consider when choosing one, download our FREE eBook:

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Suspension vs. Colloid: How Do They Differ?

Posted by Tal Shechter on Dec 20, 2017 11:30:00 AM

suspension vs colloidAs we’ve outlined in recent blogs, homogenization is a process utilized for particle size reduction and works by employing mechanical force to break down particles or droplets in a liquid into smaller and more uniform sizes. The result of homogenization is a dispersion, wherein fine particles of one substance are scattered throughout another substance. Furthermore, dispersions are able to be classified by the sizes of these scattered particles: solutions, suspensions and colloids. In this blog, we’re focusing on the difference between suspension and colloids.

Suspensions

Simply defined as a heterogeneous mixture of two substances in which one is dispersed into the other, suspensions involve particles larger than those found in solutions, typically over 1,000 nm. The bigger particles of a suspension usually settle or separate out of a mixture upon standing or are able to be filtered out, although not through filter paper. Gravity is able to pull the visible particles in a suspension down if undisturbed, and they will stay that way unless being actively mixed. Examples of suspensions include oil and water, dust or soot in air, sand and water and muddy water.

Colloids

Although a heterogeneous mixture of two substances like suspensions, colloids involve particles from 1-1,000 nm that do not separate upon standing and cannot be separated by filtration. The particles in a colloid land in size between those in a solution and a suspension and may be solid, liquid or gas. The two parts in every colloid mixture are its particles and the dispersing medium, and the particles are spread evenly in in the medium, which can also be solid, liquid or gas. Examples of colloids are foams (shaving cream, Styrofoam), gels (gelatin, jelly), emulsions (mayonnaise, lotion), aerosols (fog, insecticide spray, smoke) and sols (shampoo, gemstones).

Even though the particles in a colloid are very small in size, they can be seen through a process called the Tyndall Effect, the effect of light scattering in colloidal dispersion while showing no light in a true solution. This effect is used to determine whether a mixture is a true solution or a colloid.

Colloid mills, like homogenizers, are able to process particle reduction formulations, although each is preferential for certain applications. They are best used for samples comprised of solids immersed in a liquid suspension or a liquid suspended in another liquid because they can enhance the stability and/or reduce the size of suspended particles.

Overview

In summary, following are some of the main differences between a suspension and colloid:

  • Particles in a suspension are usually more than 1,000 nm, while those in a colloid range from 1-1,000 nm.
  • Unlike those in a suspension, particles in a colloid do not separate when sitting still.
  • The particles in a suspension may be separated by filtration unlike those in a colloid.
  • Colloids are able to scatter light, but suspensions cannot transmit light.
  • Particles in a suspension can be seen by the naked eye, but those in a colloid must be viewed using a light microscope.

BEE International: The Clear Choice for High-Pressure Homogenizers

At BEE International, we not only offer a multitude of high-pressure homogenizers to handle almost any task in a laboratory, pilot plant or industrial setting, we help you sort through the technical jargon to find equipment that best addresses your specific needs. Our line of equipment is suitable for virtually every industry and can work in just about any environment, letting you focus on other important projects. To learn more about our technology and products, please contact us today!

If you’re interested in learning more about how you can break down particles in the best way possible, download our free eBook “How to Achieve Efficient & Consistent Particle Size Reduction” today:

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Food Homogenization 101: What You Need to Know

Posted by David Shechter on Dec 13, 2017 11:30:00 AM

food homogenizationThe food and beverage industry continues to expand and diversify, especially as consumers look for more healthful products that fit both their lifestyle and budget. Companies must find ways to create and market products to differentiate them from their many competitors while ensuring their goods have the taste, freshness and nutrients on which customers have come to rely.

A common method food processing and manufacturing companies utilize to efficiently, cost-effectively and safely produce their goods is high-pressure homogenization. It not only reduces particle size through forces including high pressure, shear, turbulence, acceleration and impact but also allows for the stable and consistent production of emulsions, suspensions and other commodities that necessitate a liquid suspended in another liquid.

Though one of the most well-known uses of homogenization in the food and beverage industry is for milk, it is also widely used in the manufacture of sauces, spreads, salad dressings, fruit juices and concentrates, fish and meat paté, sweeteners, baby food, egg product, other dairy products and more. The homogenization pressures used for these products typically range between 20 and 60 MPa, but some homogenizers can reach pressures of up to 400 MPa. This magnitude of pressure leads to microbial and enzyme inactivation, which enhances the safety and shelf life of perishable foods while reducing the thermal effects on the foods’ functional and sensory qualities.

Once a company achieves its ideal product, the product needs to be reproduced on a larger scale without losing the characteristics that make it desirable by customers. If it doesn’t offer the same taste and texture on a regular basis, the manufacturer may not only lose customers and their funds but also obtain a damaged reputation.

Food homogenizers help deliver a product with uniform texture, flavoring and taste through a scalable and cost-effective process that allows for a longer period of freshness than food comprised of larger particles. The smaller the particles, the longer the structure of the product can be maintained. Food homogenizers’ ability to use pressure instead of heat preserves critical nutritional components naturally found in a food, enabling them to be available for consumption.

Although high-pressure homogenization may potentially replace thermal methods of food preparation, it does not effectively produce solid foods or ones formed from large particles. Also, because it uses force instead of thermal action, homogenization does not sterilize foods or beverages, requiring it to be accomplished through a separate process.

BEE International: Help in Finding the Best Food Homogenizer

The key to purchasing the right food homogenizer for your business needs is selecting one that can achieve consistent particle size distribution on a scalable basis. At BEE International, our equipment can engineer particle sizes at or below 100 nm, a benefit favorable for most any product in the food and beverage industry. Our products are trusted by researchers and laboratory managers around the world, and our unparalleled customer service will make finding the right product a breeze for companies of all sizes.

Contact us today to find out more about how BEE International can aid your particle size reduction process. 

For more information on the key factors to consider when choosing a cell lysis method, download our FREE eBook:

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Liquid Mixers vs High Pressure Homogenizers: Which Is Right for You?

Posted by Deb Shechter on Dec 6, 2017 11:30:00 AM

liquid mixersIt’s not uncommon for terms or even buzzwords in various industries to be similar but have different applications. For example, in the healthcare industry, electronic medical and electronic health records may sound the same. However, electronic medical records (EMRs) are a digital version of the paper charts in the clinician’s office that contain the medical and treatment history of the patients in one practice, while electronic health records (EHRs) focus on the total health of the patient, provide a broader view on a patient’s care and are designed to share information with other healthcare providers.

Similarly, though the terms “liquid homogenization” and “high-pressure homogenization” appear comparable, each has its own characteristics and primary uses. Homogenization in general can be defined as the reduction of particle size of any given molecule and is a process utilized often in the pharmaceutical, biotech, cosmetic and pharmaceutical industries. Its benefits include more even particle distribution, improved texture and enhanced appearance and taste.

Liquid homogenization, achieved by using a liquid mixer, and high pressure homogenization offer similar outcomes yet use distinct processes. The mixing processes and mixer designs chosen are determined by the nature of the materials to be mixed. Essentially, the type of product you select for your laboratory depends on its downstream applications and the cell types being used. 

In this blog, we’ll describe the differences between liquid and high-pressure homogenization and list pros and cons of each to help you decide which one is best suited to your business needs. Whichever you choose, it’s important that it has the flexibility to meet the various needs of a laboratory. 

Liquid Homogenization

Widely used for cell disruption of cultured cells and small sample volumes, liquid homogenization requires a sample to manually be forced through a narrow space, thereby shearing membranes and decreasing particle size. There are three most common types of liquid mixers used: the Dounce homogenizer (used for gently lysing single cell suspensions), Potter-Elvehjem homogenizer (used with delicate tissues) and the French press (used for small sample volumes). In addition to being suitable for operation at higher temperatures and easy to maintain, liquid mixers offer enhanced repeatability and a lower power requirement. Nevertheless, the throughput from all three models is fairly low, and they may not provide enough scalability. 

High-pressure Homogenization 

Utilized for numerous products from the chemical and biotechnology industries and products such as pharmaceutical tablet coatings, vaccinations and creams, high-pressure homogenization uses multiple mechanical forces (such as turbulence, cavitation and shear) along with intensive pressure to achieve micronization and membrane lysis. Similar to liquid mixers, high-pressure homogenizers work by forcing cell suspensions through a very narrow channel under pressure. Unlike the manual requirement of liquid homogenizers, though, high-pressure units run automatically and are often made to be scaled from laboratory experimentation to manufacturing.

Benefits of high-pressure homogenization include an increased rate of dissolution, ease of operation, cost and time effectiveness, consistency in composition of products, greater stability of final products due to a stable emulsion and upgraded scalability and repeatability. High-pressure homogenization also has the ability to reduce particle size to levels that are not achievable by most other mixing processes. Because they tend to be large and very heavy, high-pressure homogenizers are typically used for large volume processing and consume more power than liquid mixers. They sometimes require more complex cleaning, cannot handle large particles or high-fiber materials and aren’t the most suitable for high temperature materials. 

BEE: The Industry Leader in High-Pressure Homogenizers

BEE International offers a wide variety of high pressure homogenizers to meet the needs of virtually any industry. Unlike liquid mixers, our homogenizers are suited for large samples and offer large throughputs, making them ideal for laboratory, pilot plant and even industrial use. In addition, our easy-to-use equipment produces higher yield in less time and utilizes multiple forces – shear, turbulence, impact and cavitation – to allow for results that are reliable, repeatable and high quality.

There are plenty of companies on the market to select equipment from; however, the product can be of higher quality and more even consistency when run through top-shelf equipment, most frequently in the form of a homogenizer. BEE International Technologies is trusted by researchers around the world for both our laboratory homogenizers and associated customer support. Learn about how BEE’s products can maximize your homogenization processes by contacting us today. 

For more information on how to choose the right cell lysis method for your application, download our FREE eBook:

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How Do Inline Mixers Work Differently Than High-pressure Homogenizers?

Posted by Tal Shechter on Dec 1, 2017 11:30:00 AM

inline mixersWhen they hear the term “homogenization,” many people think of milk. In fact, one of the first applications of homogenization came in the processing of milk. Derived from the Greek word “homogenos” (homos meaning same and genos meaning kind), it is defined as the process of reducing the particle size of an object and is also known as micronization.

Homogenization works by forcing a sample through a narrow space through multiple mechanical forces, thereby producing a stable and consistent product. Easily scalable, it is used by multiple industries, including pharmaceutical, biotech, cosmetic and food, in order to optimize their finished product.

Under the umbrella of homogenization lie two different methods: inline mixing and high-pressure homogenization. Read on to learn the differences between how the two work and advantages associated with each.

Inline Mixing  

Positioned within the production line and aligned with the flow of product, inline mixers consist of a stationary stator and an inside rotor that turns at high speeds. The rotor–stator device is contained in a casing with an inlet at one end and an outlet at the other, and the rotor is driven through a seal. Mixing occurs when a sample is drawn in through one of the mixing heads while the blades rotate and is then expelled at a high velocity through an opening in the stator. The inline term comes into play around the mode of sample entry into the mixing chamber.

Inline mixers feed and output material in a continuous stream, allowing material to often be perfectly hydrated, emulsified or homogenized in a single pass. They differ from other types of homogenization wherein large amounts of ingredients are mixed in a standalone unit before going through the homogenization process. Designed to handle all types of tasks, including emulsifying, suspending, dispersing, blending and more, inline mixers are suitable and useful for applications where larger quantities are needed for production and continuous processing and homogenization is needed during the manufacturing process. In addition to being faster than their high-pressure counterparts, they allow for a more controlled and continuous mixing environment, improve yields, use less energy and increase capacities.

High-pressure Homogenization

Whereas inline mixers rely on rotor/stator shearing action, high-pressure homogenizers force a sample through a narrow space using force and high pressure, thereby reducing particle size or lysing cells. They consist of a high-pressure pump and a disruption unit which enable a continuous homogenization. The higher the amount of energy applied during the homogenization process, the smaller the particle size or the more complete the cell lysis. The pressure along with the action of multiple forces yields impactful results in only one or two passes. Benefits of high-pressure homogenization include consistency in composition of products, greater stability of final products and decreased cost. Also high-pressure homogenizers allow users to set operating parameters - pressure, temperature, number of passes, valve and impingement design and flow rate – for a particular homogenization.

BEE International: Your Homogenization Equipment Headquarters

At BEE International, we offer a wide selection of homogenizers that meet the needs of manufacturers across multiple industries, including cosmetic, pharmaceutical, biotech and more. Our products can help enhance your production, improve product quality and streamline your operations, all in combination with our unmatched customer service. Contact us today to learn more about our homogenization equipment and find out what product(s) is best suited to your business needs.

For more information on particle size reduction and how to achieve efficient and consistent results, download our FREE eBook:

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How to Select a Homogenizer for Chemical Applications

Posted by Deb Shechter on Oct 31, 2017 11:30:00 PM

Portrait of a smiling scientist with colleagues at work in the laboratory-280003-edited.jpegHomogenization, also known as particle size reduction or micronization, is used across many industries – including pharmaceutical, chemical, food, and cosmetic -- to create stable emulsions and dispersions. While there are a number of machines that can perform the task of homogenization, it is important to choose the right homogenizer to ensure the best result with the highest level of quality. Continue reading to learn more about some important things you should look for when choosing a homogenizer for chemical applications.  

Dispersion Creation

Dispersions, also known as suspensions, consist of solid particles distributed throughout a continuous medium. Examples of dispersions include polymers, petroleum additives, paints, and acrylics. It is important to create high quality dispersions in the production of these items to ensure maximum particle packing, increased conductivity, and improved overall surface quality.  High quality dispersions results in a higher quality end product, which almost always translates to a better bottom line.

Particle Size Reduction Capabilities

When choosing a homogenizer, it is important to choose a high pressure homogenizer that can efficiently and readily decrease particle sizes. This process, also known as micronization, results in smaller particle sizes, which help facilitate easier dissolution, increased product stability, and higher overall quality. This results in increased customer satisfaction as well as reduced costs of production.

High Pressure Homogenizers from BEE International

BEE International offers a number of high pressure homogenizers to meet the needs of virtually every industry. Our homogenizers, which include the Nano DeBEE and Micro DeBEE, just to name a few, are ideal for just about any environment, including laboratory and R&D settings, pilot plants, and industrial manufacturing zones. In addition, our homogenizers offer the following benefits and features:

  • Ability to scale to manufacturing
  • Unique processing set-ups to address specific processing challenges
  • Dual Feeds to allow for the addition of abrasive and viscous materials
  • Dual Jets that are capable for reducing particle sizes for the hardest of materials
  • Pressurized feeds that allow formation and dispersion of final products in one step
  • Increase in dense cross section of final products
  • Better particle coating during formulations

Contact us today to learn more about our high pressure homogenizers and how they can help with particle size reduction in your industry. If you’re looking to achieve particle size reduction efficiently and consistently, download our FREE eBook below:

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What are Inline Homogenizers Used For?

Posted by Tal Shechter on Oct 30, 2017 11:30:00 PM

Business man looking at wall with a bright question mark concept-222794-edited.jpegOne important question to answer when beginning any manufacturing project requiring homogenization, micronization, or particle size reduction is an obvious one: what is homogenization, and what are homogenizers used for? Finding out the answers to these two important questions is an important first step in determining the type of homogenizer to purchase and ultimately use.  Various types of homogenizers exist, all with a multitude of pros and cons. In this article, we will focus on discussing one especially popular type of homogenizer -- the Inline Homogenizer.

Inline Homogenizers are designed to handle all types of tasks, including emulsifying, suspending, dispersing, and blending. These homogenizers are used in a variety of industries, including pharmaceutical, cosmetic, biological, and chemical. What makes inline homogenizers unique? One key feature is the fact that they are positioned within the production line, aligned with the flow of product.  Inline homogenization is different from other types of homogenization, such as batch homogenization, where large amounts of ingredients are mixed in a standalone unit before going through the homogenization process.

Inline homogenizers are suitable for applications where larger quantities are needed for production. Since they are able to be installed right on the production line, they are especially useful for applications where continuous processing and homogenization is needed during the manufacturing process.

The benefits of inline homogenization are endless. Not only is inline homogenization a faster process, especially in situations where larger quantities are being produced, it is also a more economical option, since additional mixing tanks and vessels are usually not needed. Inline homogenization also ensures consistency throughout a production run, since homogenization is occurring continuously on the production line.

BEE International offers a wide variety of inline homogenizers to meet the need of virtually any industry. Our Laboratory, Pilot and Industrial Homogenizers are suitable for all production needs, from small scale R&D applications up to full scale, 24/7 industrial operations. Our DeBEE 4000 homogenizer offers the following features and benefits:

  • Complete Automation, Compliance, and Production Capacity of 120 LPH and up
  • Multiple Vertical Process Intensifiers
  • An advanced, user-friendly interface
  • CE Compliant
  • Pharma/Bio/Food Sanitary
  • Synchronized Pump System for Constant Pressure
  • Advanced Data Collection and Gathering
  • Clean in Place (CIP)
  • Programmable Maintenance Schedule with Alarms
  • In-line Spare Intensifier
  • User Management
  • Data Trending

For more information on the Inline Homogenizers in our assortment, as well as our full suite of homogenization equipment, please contact us. We are looking forward to assisting you with your next homogenization project!

Need to Efficiently & Consistently Reduce Particle Size In-Line?

Many of our manufacturing partners that use inline homogenizers need to reduce the particle size of their application in order to pass it on to the next processing stage. With a variety of particle size reduction techniques, including homogenization, how can you find the best method? Check out our free eBook “How to Achieve Efficient & Consistent Particle Size Reduction” and learn which method is best for you:

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How to Care for Your Homogenizer Equipment

Posted by Deb Shechter on Oct 27, 2017 11:30:00 AM

homogenizer equipment.jpgHomogenization, also known as particle size reduction or micronization, is used across many industries – including pharmaceutical, chemical, food, and cosmetic -- to create stable emulsions and dispersions. And while this technology has proven – and continues to prove – to be very powerful and useful, the homogenization process is only as good as the equipment that is used to undergo the process. For this reason, it is critically important to properly care for your homogenizer equipment. Continue reading to learn more about how to care for your homogenizer equipment so you can reap the benefits of its many features for years to come.

Well-cared for homogenizers can last for many years, but only if they are properly maintained. High quality homogenizers – like the ones sold by us here at BEE International – should be on a regular maintenance schedule to ensure continuous working condition. In addition, it is important that your homogenizer is not immersed in water or exposed to harmful fumes.

In addition, there are a number of preventive maintenance tasks that should be regularly completed with your homogenizer:

  • Regular replacement of motor unit brushes
  • Lubrication of bearings using a liquid sample/medium
  • Regular exterior cleaning of your homogenizer (using just soap and water is sufficient).

High Pressure Homogenizers from BEE International

BEE International offers a number of high pressure homogenizers to meet the needs of virtually every industry. Our homogenizers, which include the Nano DeBEE and Micro DeBEE, just to name a few, are ideal for just about any environment, including laboratory and R&D settings, pilot plants, and industrial manufacturing zones. In addition, our homogenizers offer the following benefits and features:

  • Ability to scale up from research and development to manufacturing
  • Unique processing set-ups to address specific processing challenges
  • Dual Feeds to allow for the addition of abrasive and viscous materials
  • Dual Jets that are capable for reducing particle sizes for the hardest of materials
  • Pressurized feeds that allow formation and dispersion of final products in one step
  • Increase in dense cross section of final products
  • Better particle coating during formulations

Contact us today to learn more about our high pressure homogenizers and how they can help with particle size reduction in your industry. If you have any additional questions about proper maintenance for your BEE Homogenizer, please don’t hesitate to reach out to a member of our team.

Learn More About Homogenizer Applications

Whether you are trying to lyse cells more efficiently or reduce particle size more consistently, we have FREE eBooks written to help you achieve both goals. Download each by clicking on their covers below:

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How to Pick Out a Homogenizer for Cell Lysis

Posted by David Shechter on Oct 26, 2017 11:30:00 PM

Thinking woman in glasses looking up with light idea bulb above head isolated on gray wall background-603464-edited.jpegCell lysis is commonly described as the process of releasing biological molecules from inside a cell, and is a powerful method utilized in a variety of manufacturing industries, including pharmaceutical, cosmetic, biotech, and R&D settings. With the popularity of this technology, more and more industry professionals are seeking out homogenizers to assist with the process of cell lysis. However, before settling on the right homogenizer for the job, it is important to understand more about cell lysis and what it entails.

During the process of cell lysis, in order to thoroughly study and analyze the behavior of molecules, the cells that produce the molecules in question must be disrupted. Disruption of cells can be achieved in numerous ways, including cryopulverization, nitrogen decompression, or through using a homogenizer.  Each method comes with varying degrees of complexity, ease, and success.

Cryopulverization is one way of accomplishing cell lysis, and is most often used for cellular samples with a tough outer matrix, such as cartilage, connective tissue, or seeds. In this method, the samples are reduced to a powder through the use of liquid nitrogen and impact pulverization. This method, while successful, is often time consuming and expensive.

Nitrogen decompression, as the name suggests, uses large quantities of nitrogen to achieve cell lysis. In this particular method, nitrogen is dissolved in the cell under very high pressures. When the pressure is released, the nitrogen violently escapes the cell, causing disruption and breakage of the cell walls. While nitrogen decompression is successful in disrupting certain types of cells – plant cells, bacteria, and other fragile cell types are most receptive to this treatment – it has not been proven to be effective in disrupting cells with tougher outer matrices, such as yeast, spores, and fungus.

Homogenization is arguably the most widely used method of cell lysis, especially for small volumes of cells and/or cultured cells. This method involves the use of shearing force on the cell. This is achieved by forcing the small cell through an even smaller sized orifice. This removes the outer layer and lyses the cell.

BEE International offers a number of high-quality homogenizers to meet the needs of any industry when it comes to the process of cell lysis and disruption. Our laboratory grade homogenizers include the Nano DeBEE, Micro DeBEE, and Mini DeBEE – all three are easy to use, offer consistent and reliable results, and easily scale up for full scale production. In addition, our homogenizers offer maximum experimentation capabilities, the ability to change flow, pressure, shear, and impact settings, and offer high pressures up to 45,000 PSI for maximum effectiveness with cell lysis and disruption.

Contact us today to learn more about how our line of homogenizers can help you meet your production needs. We are more than happy to help you get started on your next big project, and to help you choose the right homogenizer for the most efficient and effective cell lysis.

How to Choose A Cell Lysis Method

If you’re looking for more information on how to pick the best cell lysis method for your application, download our free eBook “7 Key Factors to Consider When Choosing a Cell Lysis Method”. This eBook contains practical, clear facts and insights for live science researchers and laboratory managers who want to improve existing products, enhance efficient manufacturing processes, and achieve remarkable results for new innovations. Download it here:

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