What Makes a Great Homogenizer Manufacturer?

Posted by Tal Shechter on Feb 18, 2019 2:45:58 PM

homogenizer manufacturerThere are over a hundred homogenizer manufacturers in the U.S. alone. Choosing the right one depends on your understanding of what you really need and a manufacturer’s ability to deliver it. Here’s a quick look at six features that great homogenizer manufacturers should always guarantee:

  • Consistency


It should go without saying: a great homogenizer manufacturer has a proven track record of producing high-quality machines that yield the same results time after time. Homogenizers aid processes across several industries, many of which manufacture life-altering products; companies in the pharmaceutical, food, chemical and biotechnological sectors, especially, rely on homogenizers to process products that are reliable and accurate every time.

  • Scalability


In addition, results should always scale-up from initial testing to meet final manufacturing demand. Great homogenizer manufacturers verify that their equipment produces the same results regardless of product load.

  • Efficiency


Great homogenizer manufacturers also ensure that their machines are efficient, creating a product in the fewest number of passes and/or offering accessories that improve laboratory, pilot and industrial operations when needed. The ability to customize their homogenization processes is especially important for businesses wanting to maximize budgets and save time.

  • Durability


Businesses need equipment that is durable, continuing to operate at full capacity for the longest time possible. Homogenizer manufacturers must create machines that are easy to clean, maintain and repair.

  • Safety and Security


With changing regulations and mounting privacy concerns, it’s more important than ever that businesses safeguard both their operators and their practices. Homogenizer manufacturers should implement safety features into their equipment and provide homogenizer software that is secure, programmable and responsive, assuring the privacy of data and alerting operators when maintenance needs or other issues arise.

  • Testing, Installation and Training


Finally, great homogenizer manufacturers allow opportunities for the testing of equipment before purchase and deliver ongoing customer service afterwards. A relationship with a homogenizer manufacturer should extend far beyond point of sale with warranties and support offered throughout the life of the machine.

 

Are You Ready to Learn More?

At BEE International, our patented Emulsifying Cell (EC) technology enables our homogenizers to deliver reliable, scalable results. Using multiple forces (rather than the usual one of many other homogenizer manufacturers), our equipment effectively enables high pressure pasteurization and ruptures cells, reduces particle size and creates dispersions and micro/nano emulsions. Researchers and manufacturers around the world trust our products to make everything from life-saving medicines to soup. Contact us to learn more about the other features that set us apart from the competition, making us the right homogenizer manufacturer for you, too. 
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Particle Size Reduction Equipment: Why Homogenizers Stand Out

Posted by David Shechter on Feb 9, 2019 10:00:00 AM

particle size reduction equipmentReducing large materials into smaller ones is a common and often necessary occurrence in the natural world. From the earth itself eroding to food being digested to rocks being broken into tools, the physical process of reducing something into smaller bits frequently permits advantages (or wonders!) that otherwise wouldn’t exist. Take, for instance, the splendor of the Grand Canyon or the awe-inspiring fact that our bodies can break apart food and air and water into life sustaining nutrients or the many ways humans have made valuable tools from rocks and wood and other resources. Indeed, the world’s very first tool — the hammerstone — was made by ancient hominids and served a purpose not unlike today’s own particle size reduction equipment: to turn something bigger into something smaller.

Modern particle size reduction equipment is categorized according to four basic types:

  • Crushers - which break apart large particles into smaller ones
  • Grinders - which further crumble crushed particles into powder
  • Ultrafine Grinders - which pulverize ground powder into pieces 1-50 micrometers in size
  • Cutters - which yield particles of a specific shape and size

Considering product hardness, consistency and volume (many machines are better suited for certain capacities), companies may use one or more of the equipment types. Many pharmaceutical companies, for instance, opt for ultrafine grinders that can reduce active pharmaceutical ingredients into superfine particles to increase bioavailability. Baby food and soup manufacturers, on the other hand, might need cutting capabilities to ensure that all food pieces in their products are similarly sized. Some manufacturers might use crushing techniques followed by grinding ones or run their products through one system multiple times. It really just depends on the overall purpose of the product being manufactured, the initial ingredient composition and how much money a company has to spend.

With all this in mind, savvy manufacturers started considering the use of high pressure homogenizers as a fifth alternative for businesses in need of particle size reduction equipment. Homogenizers effectively mix together two normally immiscible substances using high pressure. A happy result of the process: homogenizers also reduce particle size! For manufacturers looking to make an equipment investment that not only works, but works across numerous applications, a high pressure homogenizer has become a solid choice. Unlike traditional particle size reduction equipment that only makes use of one type of mechanical force, a high pressure homogenizer utilizes several different ones. And at BEE International, our proprietary system not only uses turbulence, cavitation, high shear and impact to break apart product, it also has modular components that can be customized for specific purposes. Turbulence and cavitation treatments can be altered, shear times can be reduced or lengthened and impact and intensity can be tweaked. So, just want does all this mean? 

The Benefits of Using High Pressure Homogenizers (HPHs) as Particle Size Reducers:

  1. HPHs produce tighter distribution of smaller particles.

  2. HPHs achieve better particle reduction in fewer passes.

  3. HPHs increase manufacturing efficiency.

  4. HPHs reduce manufacturing costs.

  5. HPHs help create products with longer shelf lives, increased bioavailability and improved sensorial and biophysical properties. 

Want to Learn More?

Homogenizers can be used for a lot more than milk production and laboratory tests. Manufacturers in nearly every industry can use them to accurately reduce product particle size with a flexibility unmatched by other equipment types. Contact our team at BEE International for additional information on the ways a high pressure homogenizer can streamline your manufacturing process, improve your product integrity and benefit your bottom line.

Please feel free to also download our free eBook, "How to Achieve Efficient & Consistent Particle Size Reduction,” for more tips.

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How to Improve Oil Emulsification with a Homogenizer

Posted by Deb Shechter on Jan 31, 2019 11:00:00 AM

oil emulsification with homogenizersEmulsification is the process by which two naturally immiscible liquids are forcefully mixed together. Salad vinaigrettes, mayonnaise, butter, cold creams, lip balms and medicated ointments are all examples of emulsions. Depending on the ratio of oil to water, emulsions are typically categorized as either oil suspended in water (o/w) or water suspended in oil (w/o). Using the right emulsifying agent and the right kind of force, you can compel either type of mixture, effectively manufacturing many textile, plastic and paper products and improving the stability, bioavailability and viability for a variety of food, cosmetic and pharmaceutical ones (among others). Take a look at how a high pressure homogenizer can improve the oil emulsification process in particular, giving you better products in less time:

But First…The Difference Between an Emulsifier and a Homogenizer

Both emulsifiers and homogenizers forcefully mix many substances. But while an emulsifier only makes use of one type of mechanical force, a homogenizer makes use of many. At BEE International, our proprietary homogenizing system combines turbulent premixing with product progression through highly pressurized spaces, utilizing shear, cavitation and impact to break apart particles and uniformly mix and blend them. The result is a product with multiple benefits.

The Benefits of Using a Homogenizer in the Oil Emulsification Process

The benefits of using a homogenizer to create emulsified products are two-fold:

  1. It Reduces Particle Size

The high pressure and additional forces used by high pressure homogenizing systems (like those with our patented BEE International Emulsifying Cell technology) drastically reduce product particle size. Sub-micro particles have more surface area per particle, which means each has more opportunity to interact with another. This not only makes it easier for mixing agents to do their job (after all, they have a larger area on which to apply their “glue”), it also creates a final product with identical (i.e., homogenous) properties, improving the texture, consistency and appearance of many consumables, (especially important for those in the food and cosmetic markets) and increasing the bioavailability of drugs for the pharmaceutical industry.

  1. It Efficiently and Effectively Mixes Resistant Substances

The vigorous and multiple forces utilized by homogenizers make it harder for product particles to resist initial mixing and harder for them to separate afterwards. It also allows for large volume processing since homogenizers have greater capacities than traditional emulsifiers. The ability to create more stable products with longer shelf lives in shorter amounts of time with fewer passes saves time and money, valuable benefits for companies wanting to make the most of their resources.

Ready To Learn More?

The value of a high pressure homogenizer extends far beyond mere mixing of substances, making it the equipment of choice for companies wanting to maximize their processes while still achieving a superior meld. If you need assistance determining if or how homogenizers can improve your manufacturing turnover, as well as provide you with superior oil emulsifications, contact our team at BEE International. Our HPP homogenizers aid all types of businesses, including those in the food, pharmaceutical, chemical, biofuel and cosmetic industries and more. 

Feel free to also download our free eBook, "How to Achieve Efficient & Consistent Particle Size Reduction," for additional information about the value of smaller particle size within a manufacturing context.

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2 Types of High Pressure Pasteurization Equipment

Posted by David Shechter on Jan 17, 2019 7:06:25 PM

two types of high pressure pasteurization equipmentIn the mid 19th century, Louis Pasteur helped France safeguard its beer and wine industries by establishing a manufacturing process that preserved and protected alcoholic products from spoilage during export. The principles of that system, eventually known as pasteurization, were based on his observations not only that microorganisms could change the molecular makeup of substances, but that they could be killed when exposed to high temperatures. Coupled with the scientific significance of French chef Nicolas Appert’s previous research on sterilization and canning techniques, thermal pasteurization quickly became the de facto approach for food manufacturers wanting to guarantee the safety and preserve the quality of their liquid and some semi-solid products.

The only problem: exposure to extreme temperatures often changes the way a food tastes, as well as its nutritional value. Believing that neither should have to be sacrificed because of safety and preservation concerns and buoyed by additional scientific advances, as well as increasing public interest in clean eating, more and more manufacturers are starting to use a high pressure processing system (HPP or pascalization) instead of heat to pasteurize their products. HPP effectively removes most pathogens and other harmful microorganisms and extends shelf life, but does not alter a food’s nutritional content, its color or its flavor. Take a look at the two most common types of high pressure pasteurization equipment: 

Water Tanks for Prepackaged Food Pasteurization

Certain prepackaged liquid and solid food items can be pasteurized using a large steel tank filled with a liquid (usually water). After the packaged food is loaded in to the tank, pumps apply pressure that changes the food’s cell structure, effectively inhibiting cell division and killing most microorganisms. According to food science research at Rutgers University, “vegetable and fruit products such as juices, salsa, dressing and guacamole; meat products such as ready-to-eat deli meats and poultry; and seafood such as shellfish and fish products” can all be processed using a high pressure pasteurization technique. The tank method is capable of processing a lot of food at one time, making it a good choice for manufacturers with high volume productions.

Homogenizers for Liquids and Semi-Solid Pasteurization

Liquids and some semi-solid products can also be pasteurized with high pressure homogenizers that push them through a series of tubes using a hydraulic pumping system that creates pressure and sterilizes them. The homogenizer then expels the product through a small opening with a drop in pressure that forces cell rupture, breaking the product apart into equally sized pieces that can be easily and uniformly mixed. Combining the pasteurization and homogenization process allows manufacturers to more efficiently manage their time, reducing their energy use and waste, and serves as a viable alternative to thermal pasteurization in the milk and juice industries especially. 

Want to Learn More?

High pressure pasteurization equipment improves the shelf life of products and maintains food flavor and nutritional value. At BEE International, our high pressure homogenizers also provide the added benefit of completely dispersing the particles of your products, allowing for uniform texture in addition to taste. This means you can use our homogenizers to enhance more than just food products. Our high pressure pasteurization equipment improves pharmaceutical, chemical, biofuel and cosmetic manufacturing and aids in RNA extraction and oil emulsification processes. Contact us to learn how it can help you, too.

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Three Reasons Cannabis Product Manufacturers Choose BEE International

Posted by Deb Shechter on Dec 27, 2018 11:30:00 AM

homogenizers for cannabis1At BEE International, we understand that quality products are reliable ones. Our high pressure homogenizers produce ingredients that make cannabis product manufacturing uniform and, thus, more effective. And in an industry where news of even one bad product or experience — whether due to poor quality or poor regulatory oversight — can give those opposed to legalized cannabis consumption the fuel needed to stoke their flames of dissent, it’s paramount that licensed cannabis manufacturers take the necessary steps to ensure that the products they deliver are of the highest quality. It’s not enough simply to get a product out there.

Cannabis manufacturers must verify both the consistency and the constancy of their products even if the market doesn’t officially demand that they do so. Take a look at three reasons cannabis product manufacturers choose to partner with BEE International:

Our Homogenizers Guarantee Efficacy

Our team at BEE International has developed cannabis-specific homogenizing processes into our equipment that effectively reduce cannabis particle size. By reducing particle size, the surface area of the cannabis increases, thereby also increasing the rates of dissolution and absorption (i.e., the bioavailabilty) of the active ingredient. This facilitates many advantages, from reducing the amount of active ingredient and time needed to produce optimal effect to stabilizing and extending shelf life for storage. These benefits translate into better products and more sales for you at the end of the day. 

Our Homogenizers Reduce Costs

Our specialized inline processes mean better results in less time. Our homogenizers establish reproducible results quickly, making it more efficient for you to validate product quality. By reducing your processing time, we can help reduce your manufacturing costs! And because our homogenizers are so good at minimizing particle size and increasing bioavailability, products made with our machines need less active ingredient to ensure superior performance. Thus, you can source less active ingredient and still produce the same great effects, saving you time and money. 

Our Homogenizers Create Stable and Uniform Particles for Quality Control

Regardless of end use, a cannabis product must be reliable and capable of being exactly reproduced. Its appearance and function must meet certain standards, with potency, appearance, consistency and even smell remaining uniform across manufacturing batches and through all moments of time. Our BEEI homogenizers help verify and maintain quality control by producing similarly sized ingredient particles that are uniform in appearance, smoother in texture and easier to mix together. We guarantee “a golden clear high performance oil” for quality products that are visually appealing and functional no matter the application. 

Want To Learn More?

Increased efficacy, lowered costs and guaranteed quality are just a few of the benefits BEE International homogenizers offer. Contact us to better understand how our proprietary processes can strengthen your cannabis products, securing you valuable market share.

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What Are Homogenizing Agents & How Do They Improve the Homogenization Process?

Posted by Deb Shechter on Dec 17, 2018 12:10:59 PM

homogenizing agentsFor most people, “homogenization” and “homogenizing agents” aren’t words that just roll off the tongue. But for us here at BEE International, talking about them comes as easily as discussing our favorite TV show or the day’s weather. So, if you’ve ever wondered what “homogenized milk” meant or how your face cream was made or why some peanut butter doesn’t have to be stirred, we’d love to share with you the facts about high pressure homogenization and homogenizing agents. Consider the following:

An Overview of the Homogenization Process

Homogenization is the process of combining two substances by breaking them into uniform pieces and mixing them together evenly. Homogenization facilitates many laboratory and manufacturing processes, such as those in the pharmaceutical, biotech, chemical, cosmetic and food industries. Not only does homogenization change the physical characteristics of materials, making it easier for scientists and manufacturers to work with raw components, it also helps ensure product quality, maintain long-term storage of materials and isolate certain particles when necessary. All of these things are necessary for superior performance among a variety of products. Homogenization makes our milk taste good and keeps our peanut butter from separating into a lump of peanut paste and a pool of oil. It keeps our lotions thick, yet spreadable, and our medicines effective.

But what if certain materials resist homogenization attempts by mechanical equipment alone? What if they need something more to help break them apart and realign their parts equally? In these cases, homogenizing agents are used.

What are Homogenizing Agents?

Homogenizing agents are resin-based mixtures which are usually added at the beginning of a homogenization cycle. When heated to certain temperatures, they turn “sticky” and help to moisten and soften the surfaces of the substances being melded together. 

How Do They Improve the Homogenization Process?

Because of their tackiness, homogenizing agents make it easier for some substances to blend and distribute their particles evenly. They also encourage filler acceptance. And by helping to shorten the time it takes to blend materials regardless of their “molecular weight, viscosity or polarity,” homogenizing agents save energy and other valuable resources.

In scientific and research settings, efficient homogenization can do more than just save time, though. It can also save lives. The right homogenizer and the right homogenizing agents can reduce the number of passes it takes to get viable results, ensuring that all steps in the testing cycle are consistent and go as smoothly and quickly as possible.

In more industrial settings, the right homogenizers and homogenizing agents ensure that applications are customized according to overall purposes, whether that be securing product stability; guaranteeing sterility; improving color, texture, taste and nutritional value; or simply maintaining cost effectiveness. 

Need More Info?

If you need help selecting the right homogenizer for your homogenization process, contact us today. Our high pressure homogenizers are customized to suit your own unique needs with multiple mechanical forces that create thoroughly mixed products.

And for additional information, you might also like to download our FREE eBook: "How to Achieve Efficient & Consistent Particle Size Reduction." In it, we show how equipment that reduces particle size saves you money by extending shelf life and strengthening biophysical properties. 

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Homogenizers for Cannabis: How To Deliver More Consistent & Uniform Particle Size

Posted by David Shechter on Nov 29, 2018 11:30:00 AM

homogenizers for cannabisIf you’re looking into cannabis processing options, homogenization might seem a bit complicated at first. While there are certain elements to homogenization that are technical, homogenization is actually a pretty simple process! When it comes to particle size reduction, homogenizers work to combine two or more ingredients in a uniform manner. Since homogenization is mechanical, it doesn’t involve adding preservatives or other materials to the mixture. Thanks to pressure and other forces, particles become uniformly dispersed in the homogenization process.

When it comes to cannabis products, one of the best benefits a homogenizer can offer is how they deliver more consistent and uniform particle sizes in their dispersions. Here are the top three reasons why this should matter to you:

1. No More “Hot” and “Cold” Spots

As mentioned above, when you homogenize two or more ingredients, those particles are reduced to a small size and uniformly dispersed throughout the mixture. So without homogenization, there’s not a good way to determine where the cannabinoids are in your products. Therefore, you may have one item that is chock full of cannabinoids while the next item doesn’t have any. In fact, a recent study found 31% of cannabis products tested did not contain the amount of cannabinoids their labels claimed. That’s an unacceptable number in any industry, but can be particularly embarrassing for companies that are looking to establish themselves in the relatively new commercial cannabis industry.

2. Increased Drug Efficacy

Next, a smaller particle size will have a greater total surface area which can increase the rates of dissolution and absorption of any active ingredient. Therefore when working with cannabis, you need to ensure your products have small, uniform particle size for a greater absorption rate and there’s no better way to do this than using a homogenizer. Homogenizers can help you reach your target particle size reliably plus you can run a filter through the end product to see that all particles are the target size. Further, smaller particles can increase bioavailability by up to 75% by increasing the surface area and you can actually reduce the active ingredient required to achieve the same effect.

3. Product Appearance

While this doesn’t apply to cannabis products alone, a smaller, more uniform particle size can be beneficial for aesthetic reasons. When particles are homogenized properly, the end product can appear smoother and more homogenous. Therefore, when looking to add cannabis to lotions, food products, and other items, having a beautiful product can make all the difference when it comes to sales.

Final Thoughts

There’s a long history of businesses across all industries using homogenizers to guarantee uniformity in their product, which is something that everyone in the cannabis industry should want when creating new products. Plus, consumers want to associate with brands and products they can trust. So when you use high-pressure homogenizers in your process, you'll be keeping the promises you already make to your customers.

To learn more about CannaBEE, our homogenizer solutions customized for cannabis, check out this page for more information or contact us today for a free quote:

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How to Improve RNA Isolation with Homogenization

Posted by David Shechter on Nov 15, 2018 11:30:00 AM

rna-isolationRNA isolation is, unsurprisingly, a delicate and complicated process that requires diligent execution and precise application. Indeed, even seemingly small mistakes can completely ruin an attempt to extract, isolate, and study RNA samples. With that in mind, today we’re here to explain how homogenization will help improve RNA isolation –– while also sharing a few best practices all professionals can use to boost their results when working with RNA. Check them out below:

Sample Collection and Preservation

Researchers value RNA analysis in no small part because studying RNA can help explain how genes function and are expressed. Regardless of the aim of a given experiment or project though, collecting and preserving samples in the right way is essential to generating viable results with RNA. Some tissues are notoriously difficult to extract RNA from (like bones or roots) and if samples aren’t preserved properly before homogenization, the results of a study can also be affected. As such, researchers should strive to either 1) conduct homogenization immediately following sample collection, which may not always be possible, or 2) freeze the samples using a substance like liquid nitrogen for temporary containment until homogenization can be performed. Note here that thawing frozen samples before homogenization will also affect the outcome of an experiment.

Benefits of Homogenization

The good news about utilizing homogenizers in regard to RNA extraction is that they eliminate the potential of heat production –– since high-pressure homogenizers operate so quickly. In addition, homogenizers are scalable and compatible with a wide array of sample sizes and types. Even if a samples has cell walls that are hard, or when other conditions aren’t ideal, homogenizers can still assist researchers in achieving meaningful yields. Lastly, homogenizers are key to separating samples from RNase, which as we’ll explain below, will significantly alter RNA samples and often render them unusable.

Preventing RNase Exposure

Ribonuclease or RNase, presents a problem for researchers looking to study intact RNA. RNase is an enzyme that catalyzes the degradation of RNA into smaller pieces. This is obviously something researchers want to avoid. Unfortunately, RNase is more or less everywhere, which means that it’s easy to expose a sample to it through lab equipment or on the surface of gloves. Professionals can use special cleaning solutions to ensure their equipment doesn’t spoil their experiment thanks to RNase exposure–– and should change their gloves often throughout their work. Remember also that proper storage at the end of the isolation procedure is also imperative to prevent any outside factors influencing results. RNA is generally stored at low temperatures –– either -20 Cº or -80 Cº depending on how long the storage will last. 

Final Thoughts

With the right equipment and by following guidelines and best practices to the letter, researchers can glean meaningful RNA yields under adverse conditions. At BEE International, we’re passionate about providing scalable, highly effective homogenization solutions for our business partners. We strive to offer the finest products –– and customer service –– in the industry. Don’t just take our word for it, though. Contact us today!

Plus, to get a firsthand look at how our homogenizers enable efficient and consistent particle-size reduction, check out our free eBook here:

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Why We Love High Pressure Homogenizers (And You Should, Too!)

Posted by David Shechter on Aug 30, 2018 11:30:00 AM

Love High Pressure HomogenizersIf you were asked about some of your favorite products, a high-pressure homogenizer may not make the list. However, as we’ve discussed in a previous blog, the homogenization process produces a lot of products you most likely use and consume in your daily life. That’s one of the reasons we here at BEE International love homogenizers…but there are more! Let’s take a look at some of the most popular uses of high-pressure homogenization and the benefits they offer consumers.

Particle Size Reduction

High-pressure homogenization utilizes mechanical force to break down particles or droplets in a liquid into smaller and more uniform sizes, resulting in a dispersion, which is where fine particles of one substance are scattered throughout another substance. Dispersions can be further classified into solutions, suspensions and colloids, a mixture somewhere between a solution and a suspension.

Some high-pressure homogenizers can reduce particle size to as small as a single-digit nanometer. Plus, they ensure the composition is equal throughout, and the overall molecular makeup of the sample isn’t compromised. Many high-pressure homogenizers are able to produce emulsions, dispersions and suspensions at particle sizes of 100 µm or higher, and a select few can achieve a size of down to 0.1 µm. Key benefits of particle size reduction include:

  • Increased dissolution rate.

  • Improved drug delivery.

  • Controlled rate of reaction.

  • Increased density.

  • Consistent texture and product appearance.

  • Enhanced sedimentation stability.

  • Improved mineral processing.


Important Applications

Food and Beverages

Though one of the most well-known uses of high-pressure homogenization in the food and beverage industry is for milk, it is also widely used in the manufacture of sauces, spreads, salad dressings, fruit juices and concentrates, fish and meat pâté, sweeteners, baby food, egg product, other dairy products and more. It helps companies utilize to efficiently, cost-effectively and safely produce their goods. Food homogenizers also aid in the delivery of a product with uniform texture, flavoring and taste through a scalable process that allows for a longer period of freshness than food comprised of larger particles.

Pharmaceuticals, Chemicals and Biofuels

High-pressure homogenizers are used for numerous products from the chemical and biotechnology industries and products such as pharmaceutical tablet coatings, vaccinations and creams. They can be used to create cost effective medicines, modify microorganisms, synthesize new chemicals and develop alternative biofuels.

Cosmetic Creams and Lotions

Due to its ability to provide potent particle size reduction, enriched texture and consistency and strengthened emulsion stability, high-pressure homogenization is a preferred method for improving the production of cosmetic creams, lotions and other cosmetic products. It is especially beneficial for beauty creams and moisturizing lotions because they have to have a combination of the right texture and consistency in order for the skin to be able to effectively absorb them.

RNA Extraction

 Utilizing homogenization for RNA extraction enables processing to be effectively and repetitively processed in only seconds, thereby eliminating heat production. Its force and high pressure produce consistent and uniform samples, and it can process both small and large samples. Plus, homogenizers are scalable and compatible with many sample types, including those with tough cell walls. Using a powerful chaotropic salt solution, RNA extraction purifies RNA from biological samples by quickly disrupting cells or tissue to release the nucleic acids from the cells. These released RNAs are valuable in providing details on which genes are expressed (used to make products), to what degree the genes are active, how they work to regulate each other via small, regulatory RNAs and which structural RNAs (i.e. rRNAs, mRNAs and tRNAs) are produced.

Oil Emulsification

 Due to the fact that water and oil are immiscible, powerful mixing is essential to conducting oil emulsification. Therefore, specialized machines such as high-pressure homogenizers are preferred because they not only impart high shear but also are able to reduce particle sizes much more efficiently than other blending and emulsification methods. They are used in a wide variety of research and scientific uses and by many industries that require the use of active ingredients because they reduce active ingredients to a small enough size to ensure a thorough dispersion throughout the medium produced. Additional benefits of high-pressure homogenization for oil emulsification include production of stable emulsions, improved product consistency, shelf-life, texture, color and flavor and the ability to overcome the resistance caused by two immiscible liquids.

BEE International: Our Love for Our Products Shows in the Way We Treat Our Customers

BEE International offers homogenizers that are both high-quality and reliable. Our line of equipment is suitable for virtually every industry and can work in just about any environment. Contact us today to learn how our products can maximize your homogenization processes.

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The Ultimate Cheat Sheet for Choosing a Cell Homogenizer

Posted by David Shechter on Aug 16, 2018 11:30:00 AM

Businessman is standing in front of two direction signs.If you attended high school before laptops and tablet computers replaced books and used a landline to make phone calls, you’ve probably heard of CliffsNotes. Often used to help students figure out the intricacies of a novel that was required reading for English class, these handy pamphlets were a sort of a study guide highlighting the work’s key points.

In this blog, we’re offering you a CliffNotes version of tips for selecting a cell homogenizer that best meets your business needs. We’ll outline cell homogenization, often referred to as cell lysis, offer key factors for how best to achieve it and provide some benefits on it.

What is Cell Lysis?

Cell lysis, which takes place in biotech, pharmaceutical, food, cosmetic and chemical laboratories on a regular basis, is the rupture of the cell membrane resulting in the release of cell contents and the subsequent death of the cell. Research scientists and laboratory technicians disrupt the cell’s structure to extract organelles, proteins, DNA or mRNA. 

Cell lysis can be achieved through mechanical or chemical methods. Mechanical cell lysis techniques include high shear mixing, traditional homogenization, sonication, grinding, freeze/thaw cycles and high-pressure homogenization. Chemical cell lysis techniques include osmotic lysis and the use of detergents, chelating agents or chaotropic agents.

In order for cell lysis to be performed successfully, the following factors should be present:

  • Intracellular proteins should be accessible for extraction and solubilization.
  • The process should be flexible, as different cells require different cell lysis strategies.
  • The technique should result in a high yield in a short time.
  • The process should be relatively easy to perform.
  • Results should be consistently reproducible and scalable.

 

How Homogenization Helps

Of the methods used to achieve cell lysis, the most widely used is homogenization, which offers a multitude of benefits for production laboratories and the consumers who buy their products. It is especially useful for small volumes of cells and/or cultured ones and involves the use of shearing force on the cell. By forcing a small cell through an even smaller-sized orifice, homogenization removes the outer layer and lyses the cell. Some cell lysis products use utilize shear, and others employ sonic cavitation. However, the best choice when purchasing a homogenizer for cell lysis is one that uses shear, cavitation and impact and lets the user adjust the relative amount of each force for different applications.

The homogenizer you choose for cell lysis should be scalable to allow for customization in pressure and intensity, resulting in both cost and time efficiency for the user. It also must accommodate for multiple cell types and adjust for various sample sizes. Other factors to consider when selecting the right cell homogenizer are the force required to produce the highest yield in the least amount of time and the volume, efficiency, stability and sanitary standards compliance of the product. Advantages of using this type of homogenizer are:

  • Accessibility of intracellular proteins.
  • Built-in process flexibility.
  • Ease-of-use and low learning curve.
  • Reproducible and scalable results.
  • Various sample size processing.
  • Ability to disrupt all cell types.
  • Better results in fewer passes.

BEEI International: Bringing the Best Homogenizers to You

BEE International offers numerous high-quality homogenizers to meet the needs of any industry for cell lysis. Our laboratory-grade models include the Nano DeBEEMicro DeBEE and Mini DeBEE, all of which are easy to use, provide consistent and reliable results and easily scale up for full scale production. Plus, they offer maximum experimentation capabilities and the ability to change flow, pressure, shear and impact settings. Contact us today to learn more about our full line of homogenizers.

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