Homogenization is an umbrella term for specific techniques that can break down and mix samples. Each technique functions optimally with distinct cell cell types and/or sample sizes. For example, liquid-based homogenization is frequently used for cell disruption of cultured cells and small sample volumes. Three types of homogenizers are well-known for their ability to process liquid homogenates; these are the Dounce homogenizer, Potter-Elvehjem homogenizer, and French press; in addition, high-pressure homogenizers can also process liquid samples. Here we present the high-pressure homogenizers manufactured by BEE International, and how they may be more effective than the traditional choices.
- Applies multiple forces
Both the Dounce and Potter-Elvehjem homogenizer use just one force, impact, to lyse cells. Somewhat better, the French press uses impact along with shear in its process. In contrast, high pressure homogenizers use a combination of multiple forces- shear, cavitation, and impact- to effectively lyse cells. This translates to better yield in fewer passes, which also confers a lower time requirement and less financial burden.
- Ability to be scaled to production
As indicated above, traditional liquid-homogenization machines are suitable for small volumes; for example, the French press can process volumes of 40-250 mL. Yet there would be few options available if that sample needed to be scaled to accommodate larger sample volumes. In contrast, Pion's BEE brand high-pressure homogenizers are known for their scaling ability, first to clinical trials and then to production. This feature is incredibly appealing to companies involved in R&D, particularly within the pharmaceutical industry.
- Process can be customized for enhanced efficiency
One of the central benefits of Pion's BEE brand high-pressure homogenizer is its flexibility, which allows scientists across industries to optimize processes like cell disruption and particle size reduction. For example, the force of cavitation is imparted once a product exits the nozzle, and contributes to the breaking of cell walls. More or less cavitation can be employed for gentler or harsher cell disruption by simply turning a dial to vary operating pressure. Similarly, the force of impact further breaks cells apart, but is only needed for samples that contain strong cell walls. When running a sample with weak walls, or lacking cell walls, a different setup can be programmed to avoid the impact step altogether. These options are not available for users of traditional liquid homogenization machines.
Pion: The Homogenizer Advantage
On determining which homogenizer will be right for your lab, begin your search with Pion. They are globally recognized among laboratory managers and researchers for their high quality products and excellent customer support. Cell lysis and particle size reduction are two of a variety of applications for Pion's BEE brand homogenizers; nano/micro emulsions, lipids, suspensions, and dispersions are also easily achievable. Additionally, the homogenizer processes can be controlled to suit your product, which will allow you to customize to your cell type. And finally, the equipment is easy to use, produces higher yield in less time, and achieves results that are reproducible and scalable.
Learn more about how Pion's BEE brand homogenizers can improve your experimentation by requesting more information.
